Reviewed, UniProtKB/Swiss-Prot Q46800 (XDHB_ECOLI)

Names and origin

Protein names

Recommended name:
    Xanthine dehydrogenase FAD-binding subunit
    EC=1.17.1.4

Gene names

Name:	xdhB
Synonyms:	ygeT
Ordered Locus Names:	b2867, JW2835

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Ref.3
Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	FAD By similarity.
Pathway	Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 2/2.
Subunit structure	Heterotrimer of xdhA, xdhB and xdhC Probable.
Sequence similarities	Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Xanthine dehydrogenase FAD-binding subunit		PRO_0000166092
Regions
	Domain	1 – 176	176	FAD-binding PCMH-type

Sequences

	Sequence		Length	Mass (Da)
	Q46800-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74A07D137DA857E8	FASTA	292	31,557
	10 20 30 40 50 60 MFDFASYHRA ATLADAINLL ADNPQAKLLA GGTDVLIQLH HHNDRYRHIV DIHNLAELRG 70 80 90 100 110 120 ITLAEDGSLR IGSATTFTQL IEDPITQRHL PALCAAATSI AGPQIRNVAT YGGNICNGAT 130 140 150 160 170 180 SADSATPTLI YDAKLEIHSP RGVRFVPING FHTGPGKVSL EHDEILVAFH FPPQPKEHAG 190 200 210 220 230 240 SAHFKYAMRD AMDISTIGCA AHCRLDNGNF SELRLAFGVA APTPIRCQHA EQTAQNAPLN 250 260 270 280 290 LQTLEAISES VLQDVAPRSS WRASKEFRLH LIQTMTKKVI SEAVAAAGGK LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage." Xi H., Schneider B.L., Reitzer L. J. Bacteriol. 182:5332-5341(2000) [PubMed: 10986234] [Abstract] Cited for: DISCUSSION OF FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28375 Genomic DNA. Translation: AAA83048.1. U00096 Genomic DNA. Translation: AAC75905.1. AP009048 Genomic DNA. Translation: BAE76933.1.
PIR	C65070.
RefSeq	AP_003427.1. NP_417343.1.
3D structure databases
HSSP	HSSP built from PDB template 1FFU based on UniProtKB P19914.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46800.
Genome annotation databases
GeneID	947205.
GenomeReviews	Gene locus JW2835 in contig AP009048_GR. Gene locus b2867 in contig U00096_GR.
KEGG	ecj:JW2835. eco:b2867.
Organism-specific databases
EchoBASE	EB2862.
EcoGene	EG13050. xdhB.
CMR	Search...
Phylogenomic databases
HOGENOM	Q46800.
OMA	GHYIKFS.
Enzyme and pathway databases
BioCyc	EcoCyc:G7486-MON. MetaCyc:G7486-MON.
Gene expression databases
Genevestigator	Q46800.
Family and domain databases
InterPro	IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR002346. Mopterin_DH_FAD-bd. [Graphical view]
Gene3D	G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
Pfam	PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. [Graphical view]
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XDHB_ECOLI

Accession

Primary (citable) accession number: Q46800
Secondary accession number(s): Q2M9X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)