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Q46800

- XDHB_ECOLI

UniProt

Q46800 - XDHB_ECOLI

Protein

Xanthine dehydrogenase FAD-binding subunit

Gene

xdhB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism).

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei165 – 1651FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei184 – 1841FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 348FADBy similarity
    Nucleotide bindingi109 – 1135FADBy similarity

    GO - Molecular functioni

    1. FAD binding Source: EcoliWiki
    2. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
    3. xanthine dehydrogenase activity Source: EcoliWiki

    GO - Biological processi

    1. hypoxanthine oxidation Source: EcoliWiki
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Purine metabolism, Purine salvage

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:G7486-MONOMER.
    ECOL316407:JW2835-MONOMER.
    MetaCyc:G7486-MONOMER.
    UniPathwayiUPA00604; UER00661.
    UPA00604; UER00662.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine dehydrogenase FAD-binding subunit (EC:1.17.1.4)
    Gene namesi
    Name:xdhB
    Synonyms:ygeT
    Ordered Locus Names:b2867, JW2835
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13050. xdhB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Xanthine dehydrogenase FAD-binding subunitPRO_0000166092Add
    BLAST

    Proteomic databases

    PaxDbiQ46800.
    PRIDEiQ46800.

    Expressioni

    Gene expression databases

    GenevestigatoriQ46800.

    Interactioni

    Subunit structurei

    Heterotrimer of XdhA, XdhB and XdhC.Curated

    Protein-protein interaction databases

    IntActiQ46800. 5 interactions.
    STRINGi511145.b2867.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46800.
    SMRiQ46800. Positions 3-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 176176FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1319.
    HOGENOMiHOG000244729.
    KOiK13479.
    OMAiIVGNICR.
    OrthoDBiEOG6ZSP3F.
    PhylomeDBiQ46800.

    Family and domain databases

    Gene3Di3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiIPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view]
    PfamiPF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view]
    SMARTiSM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46800-1 [UniParc]FASTAAdd to Basket

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    MFDFASYHRA ATLADAINLL ADNPQAKLLA GGTDVLIQLH HHNDRYRHIV    50
    DIHNLAELRG ITLAEDGSLR IGSATTFTQL IEDPITQRHL PALCAAATSI 100
    AGPQIRNVAT YGGNICNGAT SADSATPTLI YDAKLEIHSP RGVRFVPING 150
    FHTGPGKVSL EHDEILVAFH FPPQPKEHAG SAHFKYAMRD AMDISTIGCA 200
    AHCRLDNGNF SELRLAFGVA APTPIRCQHA EQTAQNAPLN LQTLEAISES 250
    VLQDVAPRSS WRASKEFRLH LIQTMTKKVI SEAVAAAGGK LQ 292
    Length:292
    Mass (Da):31,557
    Last modified:November 1, 1996 - v1
    Checksum:i74A07D137DA857E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83048.1.
    U00096 Genomic DNA. Translation: AAC75905.1.
    AP009048 Genomic DNA. Translation: BAE76933.1.
    PIRiC65070.
    RefSeqiNP_417343.1. NC_000913.3.
    YP_491069.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75905; AAC75905; b2867.
    BAE76933; BAE76933; BAE76933.
    GeneIDi12933326.
    947205.
    KEGGiecj:Y75_p2800.
    eco:b2867.
    PATRICi32121144. VBIEscCol129921_2960.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83048.1 .
    U00096 Genomic DNA. Translation: AAC75905.1 .
    AP009048 Genomic DNA. Translation: BAE76933.1 .
    PIRi C65070.
    RefSeqi NP_417343.1. NC_000913.3.
    YP_491069.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali Q46800.
    SMRi Q46800. Positions 3-285.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q46800. 5 interactions.
    STRINGi 511145.b2867.

    Proteomic databases

    PaxDbi Q46800.
    PRIDEi Q46800.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75905 ; AAC75905 ; b2867 .
    BAE76933 ; BAE76933 ; BAE76933 .
    GeneIDi 12933326.
    947205.
    KEGGi ecj:Y75_p2800.
    eco:b2867.
    PATRICi 32121144. VBIEscCol129921_2960.

    Organism-specific databases

    EchoBASEi EB2862.
    EcoGenei EG13050. xdhB.

    Phylogenomic databases

    eggNOGi COG1319.
    HOGENOMi HOG000244729.
    KOi K13479.
    OMAi IVGNICR.
    OrthoDBi EOG6ZSP3F.
    PhylomeDBi Q46800.

    Enzyme and pathway databases

    UniPathwayi UPA00604 ; UER00661 .
    UPA00604 ; UER00662 .
    BioCyci EcoCyc:G7486-MONOMER.
    ECOL316407:JW2835-MONOMER.
    MetaCyc:G7486-MONOMER.

    Miscellaneous databases

    PROi Q46800.

    Gene expression databases

    Genevestigatori Q46800.

    Family and domain databases

    Gene3Di 3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProi IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    [Graphical view ]
    Pfami PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    [Graphical view ]
    SMARTi SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55447. SSF55447. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEi PS51387. FAD_PCMH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage."
      Xi H., Schneider B.L., Reitzer L.
      J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF FUNCTION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiXDHB_ECOLI
    AccessioniPrimary (citable) accession number: Q46800
    Secondary accession number(s): Q2M9X3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3