Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative xanthine dehydrogenase FAD-binding subunit XdhB

Gene

xdhB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein predictedi

Functioni

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism).

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

FADBy similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative xanthine dehydrogenase iron-sulfur-binding subunit XdhC (xdhC), Putative xanthine dehydrogenase molybdenum-binding subunit XdhA (xdhA), Putative xanthine dehydrogenase FAD-binding subunit XdhB (xdhB)
  2. Putative xanthine dehydrogenase molybdenum-binding subunit XdhA (xdhA), Putative xanthine dehydrogenase FAD-binding subunit XdhB (xdhB)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei184FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34FADBy similarity8
Nucleotide bindingi109 – 113FADBy similarity5

GO - Molecular functioni

GO - Biological processi

  • hypoxanthine oxidation Source: EcoliWiki
  • purine ribonucleoside salvage Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism, Purine salvage
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G7486-MONOMER
MetaCyc:G7486-MONOMER
UniPathwayiUPA00604; UER00661
UPA00604; UER00662

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase FAD-binding subunit XdhB (EC:1.17.1.4)
Gene namesi
Name:xdhB
Synonyms:ygeT
Ordered Locus Names:b2867, JW2835
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13050 xdhB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660921 – 292Putative xanthine dehydrogenase FAD-binding subunit XdhBAdd BLAST292

Proteomic databases

PaxDbiQ46800
PRIDEiQ46800

Interactioni

Subunit structurei

Heterotrimer of XdhA, XdhB and XdhC.Curated

Protein-protein interaction databases

BioGridi4262320, 136 interactors
IntActiQ46800, 5 interactors
STRINGi316385.ECDH10B_3042

Structurei

3D structure databases

ProteinModelPortaliQ46800
SMRiQ46800
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 176FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST176

Phylogenomic databases

eggNOGiENOG4108HR6 Bacteria
COG1319 LUCA
HOGENOMiHOG000244729
InParanoidiQ46800
KOiK13479
OMAiMVEINFD
PhylomeDBiQ46800

Family and domain databases

InterProiView protein in InterPro
IPR005107 CO_DH_flav_C
IPR036683 CO_DH_flav_C_dom_sf
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR002346 Mopterin_DH_FAD-bd
PfamiView protein in Pfam
PF03450 CO_deh_flav_C, 1 hit
PF00941 FAD_binding_5, 1 hit
SMARTiView protein in SMART
SM01092 CO_deh_flav_C, 1 hit
SUPFAMiSSF55447 SSF55447, 1 hit
SSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

Q46800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFASYHRA ATLADAINLL ADNPQAKLLA GGTDVLIQLH HHNDRYRHIV
60 70 80 90 100
DIHNLAELRG ITLAEDGSLR IGSATTFTQL IEDPITQRHL PALCAAATSI
110 120 130 140 150
AGPQIRNVAT YGGNICNGAT SADSATPTLI YDAKLEIHSP RGVRFVPING
160 170 180 190 200
FHTGPGKVSL EHDEILVAFH FPPQPKEHAG SAHFKYAMRD AMDISTIGCA
210 220 230 240 250
AHCRLDNGNF SELRLAFGVA APTPIRCQHA EQTAQNAPLN LQTLEAISES
260 270 280 290
VLQDVAPRSS WRASKEFRLH LIQTMTKKVI SEAVAAAGGK LQ
Length:292
Mass (Da):31,557
Last modified:November 1, 1996 - v1
Checksum:i74A07D137DA857E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83048.1
U00096 Genomic DNA Translation: AAC75905.1
AP009048 Genomic DNA Translation: BAE76933.1
PIRiC65070
RefSeqiNP_417343.1, NC_000913.3
WP_000459182.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75905; AAC75905; b2867
BAE76933; BAE76933; BAE76933
GeneIDi947205
KEGGiecj:JW2835
eco:b2867
PATRICifig|1411691.4.peg.3867

Entry informationi

Entry nameiXDHB_ECOLI
AccessioniPrimary (citable) accession number: Q46800
Secondary accession number(s): Q2M9X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health