Q46800 (XDHB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine dehydrogenase FAD-binding subunit
EC=1.17.1.4

Gene names

Name:	xdhB
Synonyms:	ygeT
Ordered Locus Names:	b2867, JW2835

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Ref.3
Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	FAD By similarity.
Pathway	Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 2/2.
Subunit structure	Heterotrimer of XdhA, XdhB and XdhC Probable.
Sequence similarities	Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hypoxanthine oxidation Inferred from mutant phenotype Ref.3. Source: EcoliWiki purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FAD binding Inferred from sequence alignment Ref.3. Source: EcoliWiki UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro xanthine dehydrogenase activity Inferred from sequence alignment Ref.3. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Xanthine dehydrogenase FAD-binding subunit		PRO_0000166092
Regions
	Domain	1 – 176	176	FAD-binding PCMH-type
	Nucleotide binding	27 – 34	8	FAD By similarity
	Nucleotide binding	109 – 113	5	FAD By similarity
Sites
	Binding site	165	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	184	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46800 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74A07D137DA857E8	FASTA	292	31,557
	10 20 30 40 50 60 MFDFASYHRA ATLADAINLL ADNPQAKLLA GGTDVLIQLH HHNDRYRHIV DIHNLAELRG 70 80 90 100 110 120 ITLAEDGSLR IGSATTFTQL IEDPITQRHL PALCAAATSI AGPQIRNVAT YGGNICNGAT 130 140 150 160 170 180 SADSATPTLI YDAKLEIHSP RGVRFVPING FHTGPGKVSL EHDEILVAFH FPPQPKEHAG 190 200 210 220 230 240 SAHFKYAMRD AMDISTIGCA AHCRLDNGNF SELRLAFGVA APTPIRCQHA EQTAQNAPLN 250 260 270 280 290 LQTLEAISES VLQDVAPRSS WRASKEFRLH LIQTMTKKVI SEAVAAAGGK LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage." Xi H., Schneider B.L., Reitzer L. J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28375 Genomic DNA. Translation: AAA83048.1. U00096 Genomic DNA. Translation: AAC75905.1. AP009048 Genomic DNA. Translation: BAE76933.1.
PIR	C65070.
RefSeq	NP_417343.1. NC_000913.2. YP_491069.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46800.
SMR	Q46800. Positions 3-285.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q46800. 5 interactions.
STRING	511145.b2867.
Proteomic databases
PaxDb	Q46800.
PRIDE	Q46800.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75905; AAC75905; b2867. BAE76933; BAE76933; BAE76933.
GeneID	12933326. 947205.
KEGG	ecj:Y75_p2800. eco:b2867.
PATRIC	32121144. VBIEscCol129921_2960.
Organism-specific databases
EchoBASE	EB2862.
EcoGene	EG13050. xdhB.
Phylogenomic databases
eggNOG	COG1319.
HOGENOM	HOG000244729.
KO	K13479.
OMA	IEDPVTQ.
ProtClustDB	PRK09971.
Enzyme and pathway databases
BioCyc	EcoCyc:G7486-MONOMER. ECOL316407:JW2835-MONOMER. MetaCyc:G7486-MONOMER.
UniPathway	UPA00604; UER00661. UPA00604; UER00662.
Gene expression databases
Genevestigator	Q46800.
Family and domain databases
Gene3D	3.30.43.10. 1 hit. 3.30.465.10. 1 hit.
InterPro	IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR002346. Mopterin_DH_FAD-bd. [Graphical view]
Pfam	PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. [Graphical view]
SMART	SM01092. CO_deh_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF55447. CO_deh_flav_C. 1 hit. SSF56176. FAD-binding_2. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XDHB_ECOLI

Accession

Primary (citable) accession number: Q46800
Secondary accession number(s): Q2M9X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program