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Q46799

- XDHA_ECOLI

UniProt

Q46799 - XDHA_ECOLI

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Protein
Xanthine dehydrogenase molybdenum-binding subunit
Gene
xdhA, ygeS, b2866, JW5462
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi206 – 2061Molybdenum By similarity
Metal bindingi237 – 2371Molybdenum; via carbonyl oxygen By similarity
Metal bindingi350 – 3501Molybdenum; via amide nitrogen By similarity
Metal bindingi516 – 5161Molybdenum; via amide nitrogen By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. oxidoreductase activity Source: EcoliWiki
  3. xanthine dehydrogenase activity Source: EcoCyc

GO - Biological processi

  1. hypoxanthine catabolic process Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G7485-MONOMER.
ECOL316407:JW5462-MONOMER.
MetaCyc:G7485-MONOMER.
UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase molybdenum-binding subunit (EC:1.17.1.4)
Gene namesi
Name:xdhA
Synonyms:ygeS
Ordered Locus Names:b2866, JW5462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13049. xdhA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Xanthine dehydrogenase molybdenum-binding subunit
PRO_0000166088Add
BLAST

Proteomic databases

PaxDbiQ46799.
PRIDEiQ46799.

Expressioni

Inductioni

Is not solely regulated by nitrogen limitation.

Gene expression databases

GenevestigatoriQ46799.

Interactioni

Subunit structurei

Heterotrimer of XdhA, XdhB and XdhC Inferred.

Protein-protein interaction databases

IntActiQ46799. 1 interaction.
STRINGi511145.b2866.

Structurei

3D structure databases

ProteinModelPortaliQ46799.
SMRiQ46799. Positions 1-746.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1529.
HOGENOMiHOG000244715.
KOiK00087.
OMAiAYVSHGH.
OrthoDBiEOG6ZSP3F.
PhylomeDBiQ46799.

Family and domain databases

Gene3Di3.30.365.10. 6 hits.
3.90.1170.50. 1 hit.
InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view]
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46799-1 [UniParc]FASTAAdd to Basket

« Hide

MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL    50
PGVLAIFTWE DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV 100
AIVVARDELT AEKAAQLVSI EWQELPVITT PEAALAEDAA PIHNGGNLLK 150
QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ HCHMESVTSL AWMEDDSRIT 200
IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK QDVLEEPMAA 250
FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD 300
VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR 350
GYGAPQVVFA VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS 400
AGLPECLEKG RKIFEWEKRR AECQNQQGNL RRGVGVACFS YTSNTWPVGV 450
EIAGARLLMN QDGTINVQSG ATEIGQGADT VFSQMVAETV GVPVSDVRVI 500
STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA HAAVMLHQSA 550
MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT 600
NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG 650
MGIGWALFEE MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ 700
SAYGHKSLGE PPIIPVAAAI RNAVKMATGV AINTLPLTPK RLYEEFHLAG 750
LI 752
Length:752
Mass (Da):81,321
Last modified:November 1, 1996 - v1
Checksum:iDD16E1FAA8497AC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28375 Genomic DNA. Translation: AAA83047.1.
U00096 Genomic DNA. Translation: AAC75904.1.
AP009048 Genomic DNA. Translation: BAE76932.1.
PIRiB65070.
RefSeqiNP_417342.1. NC_000913.3.
YP_491068.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75904; AAC75904; b2866.
BAE76932; BAE76932; BAE76932.
GeneIDi12933325.
947116.
KEGGiecj:Y75_p2799.
eco:b2866.
PATRICi32121142. VBIEscCol129921_2959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28375 Genomic DNA. Translation: AAA83047.1 .
U00096 Genomic DNA. Translation: AAC75904.1 .
AP009048 Genomic DNA. Translation: BAE76932.1 .
PIRi B65070.
RefSeqi NP_417342.1. NC_000913.3.
YP_491068.1. NC_007779.1.

3D structure databases

ProteinModelPortali Q46799.
SMRi Q46799. Positions 1-746.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q46799. 1 interaction.
STRINGi 511145.b2866.

Proteomic databases

PaxDbi Q46799.
PRIDEi Q46799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75904 ; AAC75904 ; b2866 .
BAE76932 ; BAE76932 ; BAE76932 .
GeneIDi 12933325.
947116.
KEGGi ecj:Y75_p2799.
eco:b2866.
PATRICi 32121142. VBIEscCol129921_2959.

Organism-specific databases

EchoBASEi EB2861.
EcoGenei EG13049. xdhA.

Phylogenomic databases

eggNOGi COG1529.
HOGENOMi HOG000244715.
KOi K00087.
OMAi AYVSHGH.
OrthoDBi EOG6ZSP3F.
PhylomeDBi Q46799.

Enzyme and pathway databases

UniPathwayi UPA00604 ; UER00661 .
UPA00604 ; UER00662 .
BioCyci EcoCyc:G7485-MONOMER.
ECOL316407:JW5462-MONOMER.
MetaCyc:G7485-MONOMER.

Miscellaneous databases

PROi Q46799.

Gene expression databases

Genevestigatori Q46799.

Family and domain databases

Gene3Di 3.30.365.10. 6 hits.
3.90.1170.50. 1 hit.
InterProi IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view ]
Pfami PF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view ]
SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view ]
SUPFAMi SSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage."
    Xi H., Schneider B.L., Reitzer L.
    J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiXDHA_ECOLI
AccessioniPrimary (citable) accession number: Q46799
Secondary accession number(s): Q2M9X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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