Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Xanthine dehydrogenase molybdenum-binding subunit

Gene

xdhA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi206 – 2061MolybdenumBy similarity
Metal bindingi237 – 2371Molybdenum; via carbonyl oxygenBy similarity
Metal bindingi350 – 3501Molybdenum; via amide nitrogenBy similarity
Metal bindingi516 – 5161Molybdenum; via amide nitrogenBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: GO_Central
  2. metal ion binding Source: UniProtKB-KW
  3. molybdopterin cofactor binding Source: GO_Central
  4. oxidoreductase activity Source: EcoliWiki
  5. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: GO_Central
  6. xanthine dehydrogenase activity Source: EcoCyc
  7. xanthine oxidase activity Source: GO_Central

GO - Biological processi

  1. hypoxanthine catabolic process Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
  3. xanthine catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism, Purine salvage

Keywords - Ligandi

Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G7485-MONOMER.
ECOL316407:JW5462-MONOMER.
MetaCyc:G7485-MONOMER.
UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase molybdenum-binding subunit (EC:1.17.1.4)
Gene namesi
Name:xdhA
Synonyms:ygeS
Ordered Locus Names:b2866, JW5462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13049. xdhA.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Xanthine dehydrogenase molybdenum-binding subunitPRO_0000166088Add
BLAST

Proteomic databases

PaxDbiQ46799.
PRIDEiQ46799.

Expressioni

Inductioni

Is not solely regulated by nitrogen limitation.

Gene expression databases

GenevestigatoriQ46799.

Interactioni

Subunit structurei

Heterotrimer of XdhA, XdhB and XdhC.Curated

Protein-protein interaction databases

IntActiQ46799. 1 interaction.
STRINGi511145.b2866.

Structurei

3D structure databases

ProteinModelPortaliQ46799.
SMRiQ46799. Positions 1-746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1529.
HOGENOMiHOG000244715.
InParanoidiQ46799.
KOiK00087.
OMAiAYVSHGH.
OrthoDBiEOG6ZSP3F.
PhylomeDBiQ46799.

Family and domain databases

Gene3Di3.30.365.10. 6 hits.
3.90.1170.50. 1 hit.
InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view]
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL
60 70 80 90 100
PGVLAIFTWE DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV
110 120 130 140 150
AIVVARDELT AEKAAQLVSI EWQELPVITT PEAALAEDAA PIHNGGNLLK
160 170 180 190 200
QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ HCHMESVTSL AWMEDDSRIT
210 220 230 240 250
IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK QDVLEEPMAA
260 270 280 290 300
FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD
310 320 330 340 350
VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR
360 370 380 390 400
GYGAPQVVFA VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS
410 420 430 440 450
AGLPECLEKG RKIFEWEKRR AECQNQQGNL RRGVGVACFS YTSNTWPVGV
460 470 480 490 500
EIAGARLLMN QDGTINVQSG ATEIGQGADT VFSQMVAETV GVPVSDVRVI
510 520 530 540 550
STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA HAAVMLHQSA
560 570 580 590 600
MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT
610 620 630 640 650
NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG
660 670 680 690 700
MGIGWALFEE MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ
710 720 730 740 750
SAYGHKSLGE PPIIPVAAAI RNAVKMATGV AINTLPLTPK RLYEEFHLAG

LI
Length:752
Mass (Da):81,321
Last modified:November 1, 1996 - v1
Checksum:iDD16E1FAA8497AC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83047.1.
U00096 Genomic DNA. Translation: AAC75904.1.
AP009048 Genomic DNA. Translation: BAE76932.1.
PIRiB65070.
RefSeqiNP_417342.1. NC_000913.3.
YP_491068.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75904; AAC75904; b2866.
BAE76932; BAE76932; BAE76932.
GeneIDi12933325.
947116.
KEGGiecj:Y75_p2799.
eco:b2866.
PATRICi32121142. VBIEscCol129921_2959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83047.1.
U00096 Genomic DNA. Translation: AAC75904.1.
AP009048 Genomic DNA. Translation: BAE76932.1.
PIRiB65070.
RefSeqiNP_417342.1. NC_000913.3.
YP_491068.1. NC_007779.1.

3D structure databases

ProteinModelPortaliQ46799.
SMRiQ46799. Positions 1-746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ46799. 1 interaction.
STRINGi511145.b2866.

Proteomic databases

PaxDbiQ46799.
PRIDEiQ46799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75904; AAC75904; b2866.
BAE76932; BAE76932; BAE76932.
GeneIDi12933325.
947116.
KEGGiecj:Y75_p2799.
eco:b2866.
PATRICi32121142. VBIEscCol129921_2959.

Organism-specific databases

EchoBASEiEB2861.
EcoGeneiEG13049. xdhA.

Phylogenomic databases

eggNOGiCOG1529.
HOGENOMiHOG000244715.
InParanoidiQ46799.
KOiK00087.
OMAiAYVSHGH.
OrthoDBiEOG6ZSP3F.
PhylomeDBiQ46799.

Enzyme and pathway databases

UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.
BioCyciEcoCyc:G7485-MONOMER.
ECOL316407:JW5462-MONOMER.
MetaCyc:G7485-MONOMER.

Miscellaneous databases

PROiQ46799.

Gene expression databases

GenevestigatoriQ46799.

Family and domain databases

Gene3Di3.30.365.10. 6 hits.
3.90.1170.50. 1 hit.
InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view]
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage."
    Xi H., Schneider B.L., Reitzer L.
    J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiXDHA_ECOLI
AccessioniPrimary (citable) accession number: Q46799
Secondary accession number(s): Q2M9X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.