Q46799 (XDHA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine dehydrogenase molybdenum-binding subunit
EC=1.17.1.4

Gene names

Name:	xdhA
Synonyms:	ygeS
Ordered Locus Names:	b2866, JW5462

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage. Ref.3
Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	Binds 1 molybdenum ion (molybdopterin) per subunit By similarity.
Pathway	Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 2/2.
Subunit structure	Heterotrimer of XdhA, XdhB and XdhC Probable.
Induction	Is not solely regulated by nitrogen limitation.
Sequence similarities	Belongs to the xanthine dehydrogenase family.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hypoxanthine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW xanthine dehydrogenase activity Inferred from genetic interaction Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	Xanthine dehydrogenase molybdenum-binding subunit		PRO_0000166088
Sites
	Metal binding	206	1	Molybdenum By similarity
	Metal binding	237	1	Molybdenum; via carbonyl oxygen By similarity
	Metal binding	350	1	Molybdenum; via amide nitrogen By similarity
	Metal binding	516	1	Molybdenum; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46799 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DD16E1FAA8497AC4	FASTA	752	81,321
	10 20 30 40 50 60 MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL PGVLAIFTWE 70 80 90 100 110 120 DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV AIVVARDELT AEKAAQLVSI 130 140 150 160 170 180 EWQELPVITT PEAALAEDAA PIHNGGNLLK QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ 190 200 210 220 230 240 HCHMESVTSL AWMEDDSRIT IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK 250 260 270 280 290 300 QDVLEEPMAA FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD 310 320 330 340 350 360 VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR GYGAPQVVFA 370 380 390 400 410 420 VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS AGLPECLEKG RKIFEWEKRR 430 440 450 460 470 480 AECQNQQGNL RRGVGVACFS YTSNTWPVGV EIAGARLLMN QDGTINVQSG ATEIGQGADT 490 500 510 520 530 540 VFSQMVAETV GVPVSDVRVI STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA 550 560 570 580 590 600 HAAVMLHQSA MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT 610 620 630 640 650 660 NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG MGIGWALFEE 670 680 690 700 710 720 MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ SAYGHKSLGE PPIIPVAAAI 730 740 750 RNAVKMATGV AINTLPLTPK RLYEEFHLAG LI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage." Xi H., Schneider B.L., Reitzer L. J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28375 Genomic DNA. Translation: AAA83047.1. U00096 Genomic DNA. Translation: AAC75904.1. AP009048 Genomic DNA. Translation: BAE76932.1.
PIR	B65070.
RefSeq	NP_417342.1. NC_000913.2. YP_491068.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46799.
SMR	Q46799. Positions 1-746.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q46799. 1 interaction.
STRING	511145.b2866.
Proteomic databases
PaxDb	Q46799.
PRIDE	Q46799.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75904; AAC75904; b2866. BAE76932; BAE76932; BAE76932.
GeneID	12933325. 947116.
KEGG	ecj:Y75_p2799. eco:b2866.
PATRIC	32121142. VBIEscCol129921_2959.
Organism-specific databases
EchoBASE	EB2861.
EcoGene	EG13049. xdhA.
Phylogenomic databases
eggNOG	COG1529.
HOGENOM	HOG000244715.
KO	K00087.
OMA	SREGDAN.
ProtClustDB	PRK09970.
Enzyme and pathway databases
BioCyc	EcoCyc:G7485-MONOMER. ECOL316407:JW5462-MONOMER. MetaCyc:G7485-MONOMER.
UniPathway	UPA00604; UER00661. UPA00604; UER00662.
Gene expression databases
Genevestigator	Q46799.
Family and domain databases
Gene3D	3.30.365.10. 6 hits. 3.90.1170.50. 1 hit.
InterPro	IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. [Graphical view]
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. [Graphical view]
SMART	SM01008. Ald_Xan_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF56003. Ald_xan_DH_mo_bd. 1 hit. SSF54665. Aldxan_dh_hamm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XDHA_ECOLI

Accession

Primary (citable) accession number: Q46799
Secondary accession number(s): Q2M9X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program