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Protein

Putative xanthine dehydrogenase molybdenum-binding subunit XdhA

Gene

xdhA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative xanthine dehydrogenase iron-sulfur-binding subunit XdhC (xdhC), Putative xanthine dehydrogenase molybdenum-binding subunit XdhA (xdhA), Putative xanthine dehydrogenase FAD-binding subunit XdhB (xdhB)
  2. Putative xanthine dehydrogenase molybdenum-binding subunit XdhA (xdhA), Putative xanthine dehydrogenase FAD-binding subunit XdhB (xdhB)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi206MolybdenumBy similarity1
Metal bindingi237Molybdenum; via carbonyl oxygenBy similarity1
Metal bindingi350Molybdenum; via amide nitrogenBy similarity1
Metal bindingi516Molybdenum; via amide nitrogenBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity Source: EcoliWiki
  • xanthine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism, Purine salvage
LigandMetal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G7485-MONOMER
MetaCyc:G7485-MONOMER
UniPathwayiUPA00604; UER00661
UPA00604; UER00662

Names & Taxonomyi

Protein namesi
Recommended name:
Putative xanthine dehydrogenase molybdenum-binding subunit XdhA (EC:1.17.1.4)
Gene namesi
Name:xdhA
Synonyms:ygeS
Ordered Locus Names:b2866, JW5462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13049 xdhA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660881 – 752Putative xanthine dehydrogenase molybdenum-binding subunit XdhAAdd BLAST752

Proteomic databases

PaxDbiQ46799
PRIDEiQ46799

Expressioni

Inductioni

Is not solely regulated by nitrogen limitation.

Interactioni

Subunit structurei

Heterotrimer of XdhA, XdhB and XdhC.Curated

Protein-protein interaction databases

BioGridi4262319, 8 interactors
IntActiQ46799, 1 interactor
STRINGi316385.ECDH10B_3041

Structurei

3D structure databases

ProteinModelPortaliQ46799
SMRiQ46799
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C7W Bacteria
COG1529 LUCA
HOGENOMiHOG000244715
InParanoidiQ46799
KOiK00087
OMAiPYNPVAP
PhylomeDBiQ46799

Family and domain databases

InterProiView protein in InterPro
IPR000674 Ald_Oxase/Xan_DH_a/b
IPR036856 Ald_Oxase/Xan_DH_a/b_sf
IPR008274 AldOxase/xan_DH_Mopterin-bd
IPR037165 AldOxase/xan_DH_Mopterin-bd_sf
PfamiView protein in Pfam
PF01315 Ald_Xan_dh_C, 1 hit
PF02738 Ald_Xan_dh_C2, 1 hit
SMARTiView protein in SMART
SM01008 Ald_Xan_dh_C, 1 hit
SUPFAMiSSF54665 SSF54665, 1 hit
SSF56003 SSF56003, 1 hit

Sequencei

Sequence statusi: Complete.

Q46799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL
60 70 80 90 100
PGVLAIFTWE DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV
110 120 130 140 150
AIVVARDELT AEKAAQLVSI EWQELPVITT PEAALAEDAA PIHNGGNLLK
160 170 180 190 200
QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ HCHMESVTSL AWMEDDSRIT
210 220 230 240 250
IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK QDVLEEPMAA
260 270 280 290 300
FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD
310 320 330 340 350
VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR
360 370 380 390 400
GYGAPQVVFA VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS
410 420 430 440 450
AGLPECLEKG RKIFEWEKRR AECQNQQGNL RRGVGVACFS YTSNTWPVGV
460 470 480 490 500
EIAGARLLMN QDGTINVQSG ATEIGQGADT VFSQMVAETV GVPVSDVRVI
510 520 530 540 550
STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA HAAVMLHQSA
560 570 580 590 600
MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT
610 620 630 640 650
NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG
660 670 680 690 700
MGIGWALFEE MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ
710 720 730 740 750
SAYGHKSLGE PPIIPVAAAI RNAVKMATGV AINTLPLTPK RLYEEFHLAG

LI
Length:752
Mass (Da):81,321
Last modified:November 1, 1996 - v1
Checksum:iDD16E1FAA8497AC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83047.1
U00096 Genomic DNA Translation: AAC75904.1
AP009048 Genomic DNA Translation: BAE76932.1
PIRiB65070
RefSeqiNP_417342.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC75904; AAC75904; b2866
BAE76932; BAE76932; BAE76932
GeneIDi947116
KEGGiecj:JW5462
eco:b2866
PATRICifig|511145.12.peg.2959

Similar proteinsi

Entry informationi

Entry nameiXDHA_ECOLI
AccessioniPrimary (citable) accession number: Q46799
Secondary accession number(s): Q2M9X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health