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Q46799 (XDHA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xanthine dehydrogenase molybdenum-binding subunit

EC=1.17.1.4
Gene names
Name:xdhA
Synonyms:ygeS
Ordered Locus Names:b2866, JW5462
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length752 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage. Ref.3

Catalytic activity

Xanthine + NAD+ + H2O = urate + NADH.

Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactor

Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Pathway

Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 1/2.

Purine metabolism; hypoxanthine degradation; urate from hypoxanthine: step 2/2.

Subunit structure

Heterotrimer of XdhA, XdhB and XdhC Probable.

Induction

Is not solely regulated by nitrogen limitation.

Sequence similarities

Belongs to the xanthine dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 752752Xanthine dehydrogenase molybdenum-binding subunit
PRO_0000166088

Sites

Metal binding2061Molybdenum By similarity
Metal binding2371Molybdenum; via carbonyl oxygen By similarity
Metal binding3501Molybdenum; via amide nitrogen By similarity
Metal binding5161Molybdenum; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46799 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DD16E1FAA8497AC4

FASTA75281,321
        10         20         30         40         50         60 
MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL PGVLAIFTWE 

        70         80         90        100        110        120 
DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV AIVVARDELT AEKAAQLVSI 

       130        140        150        160        170        180 
EWQELPVITT PEAALAEDAA PIHNGGNLLK QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ 

       190        200        210        220        230        240 
HCHMESVTSL AWMEDDSRIT IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK 

       250        260        270        280        290        300 
QDVLEEPMAA FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD 

       310        320        330        340        350        360 
VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR GYGAPQVVFA 

       370        380        390        400        410        420 
VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS AGLPECLEKG RKIFEWEKRR 

       430        440        450        460        470        480 
AECQNQQGNL RRGVGVACFS YTSNTWPVGV EIAGARLLMN QDGTINVQSG ATEIGQGADT 

       490        500        510        520        530        540 
VFSQMVAETV GVPVSDVRVI STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA 

       550        560        570        580        590        600 
HAAVMLHQSA MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT 

       610        620        630        640        650        660 
NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG MGIGWALFEE 

       670        680        690        700        710        720 
MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ SAYGHKSLGE PPIIPVAAAI 

       730        740        750 
RNAVKMATGV AINTLPLTPK RLYEEFHLAG LI 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage."
Xi H., Schneider B.L., Reitzer L.
J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28375 Genomic DNA. Translation: AAA83047.1.
U00096 Genomic DNA. Translation: AAC75904.1.
AP009048 Genomic DNA. Translation: BAE76932.1.
PIRB65070.
RefSeqNP_417342.1. NC_000913.3.
YP_491068.1. NC_007779.1.

3D structure databases

ProteinModelPortalQ46799.
SMRQ46799. Positions 1-746.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ46799. 1 interaction.
STRING511145.b2866.

Proteomic databases

PaxDbQ46799.
PRIDEQ46799.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75904; AAC75904; b2866.
BAE76932; BAE76932; BAE76932.
GeneID12933325.
947116.
KEGGecj:Y75_p2799.
eco:b2866.
PATRIC32121142. VBIEscCol129921_2959.

Organism-specific databases

EchoBASEEB2861.
EcoGeneEG13049. xdhA.

Phylogenomic databases

eggNOGCOG1529.
HOGENOMHOG000244715.
KOK00087.
OMAAYVSHGH.
OrthoDBEOG6ZSP3F.
PhylomeDBQ46799.

Enzyme and pathway databases

BioCycEcoCyc:G7485-MONOMER.
ECOL316407:JW5462-MONOMER.
MetaCyc:G7485-MONOMER.
UniPathwayUPA00604; UER00661.
UPA00604; UER00662.

Gene expression databases

GenevestigatorQ46799.

Family and domain databases

Gene3D3.30.365.10. 6 hits.
3.90.1170.50. 1 hit.
InterProIPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view]
PfamPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view]
SMARTSM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view]
SUPFAMSSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.
ProtoNetSearch...

Other

PROQ46799.

Entry information

Entry nameXDHA_ECOLI
AccessionPrimary (citable) accession number: Q46799
Secondary accession number(s): Q2M9X4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene