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Q46799

- XDHA_ECOLI

UniProt

Q46799 - XDHA_ECOLI

Protein

Xanthine dehydrogenase molybdenum-binding subunit

Gene

xdhA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism). Deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage.1 Publication

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.

    Cofactori

    Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi206 – 2061MolybdenumBy similarity
    Metal bindingi237 – 2371Molybdenum; via carbonyl oxygenBy similarity
    Metal bindingi350 – 3501Molybdenum; via amide nitrogenBy similarity
    Metal bindingi516 – 5161Molybdenum; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. oxidoreductase activity Source: EcoliWiki
    3. xanthine dehydrogenase activity Source: EcoCyc

    GO - Biological processi

    1. hypoxanthine catabolic process Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Purine metabolism, Purine salvage

    Keywords - Ligandi

    Metal-binding, Molybdenum, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:G7485-MONOMER.
    ECOL316407:JW5462-MONOMER.
    MetaCyc:G7485-MONOMER.
    UniPathwayiUPA00604; UER00661.
    UPA00604; UER00662.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine dehydrogenase molybdenum-binding subunit (EC:1.17.1.4)
    Gene namesi
    Name:xdhA
    Synonyms:ygeS
    Ordered Locus Names:b2866, JW5462
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13049. xdhA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 752752Xanthine dehydrogenase molybdenum-binding subunitPRO_0000166088Add
    BLAST

    Proteomic databases

    PaxDbiQ46799.
    PRIDEiQ46799.

    Expressioni

    Inductioni

    Is not solely regulated by nitrogen limitation.

    Gene expression databases

    GenevestigatoriQ46799.

    Interactioni

    Subunit structurei

    Heterotrimer of XdhA, XdhB and XdhC.Curated

    Protein-protein interaction databases

    IntActiQ46799. 1 interaction.
    STRINGi511145.b2866.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46799.
    SMRiQ46799. Positions 1-746.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the xanthine dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1529.
    HOGENOMiHOG000244715.
    KOiK00087.
    OMAiAYVSHGH.
    OrthoDBiEOG6ZSP3F.
    PhylomeDBiQ46799.

    Family and domain databases

    Gene3Di3.30.365.10. 6 hits.
    3.90.1170.50. 1 hit.
    InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    [Graphical view]
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54665. SSF54665. 1 hit.
    SSF56003. SSF56003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVDAIAKVT GRARYTDDYV MAGMCYAKYV RSPIAHGYAV SINDEQARSL    50
    PGVLAIFTWE DVPDIPFATA GHAWTLDENK RDTADRALLT RHVRHHGDAV 100
    AIVVARDELT AEKAAQLVSI EWQELPVITT PEAALAEDAA PIHNGGNLLK 150
    QSTMSTGNVQ QTIDAADYQV QGHYQTPVIQ HCHMESVTSL AWMEDDSRIT 200
    IVSSTQIPHI VRRVVGQALD IPWSCVRVIK PFVGGGFGNK QDVLEEPMAA 250
    FLTSKLGGIP VKVSLSREEC FLATRTRHAF TIDGQMGVNR DGTLKGYSLD 300
    VLSNTGAYAS HGHSIASAGG NKVAYLYPRC AYAYSSKTCY TNLPSAGAMR 350
    GYGAPQVVFA VESMLDDAAT ALGIDPVEIR LRNAAREGDA NPLTGKRIYS 400
    AGLPECLEKG RKIFEWEKRR AECQNQQGNL RRGVGVACFS YTSNTWPVGV 450
    EIAGARLLMN QDGTINVQSG ATEIGQGADT VFSQMVAETV GVPVSDVRVI 500
    STQDTDVTPF DPGAFASRQS YVAAPALRSA ALLLKEKIIA HAAVMLHQSA 550
    MNLTLIKGHI VLVERPEEPL MSLKDLAMDA FYHPERGGQL SAESSIKTTT 600
    NPPAFGCTFV DLTVDIALCK VTINRILNVH DSGHILNPLL AEGQVHGGMG 650
    MGIGWALFEE MIIDAKSGVV RNPNLLDYKM PTMPDLPQLE SAFVEINEPQ 700
    SAYGHKSLGE PPIIPVAAAI RNAVKMATGV AINTLPLTPK RLYEEFHLAG 750
    LI 752
    Length:752
    Mass (Da):81,321
    Last modified:November 1, 1996 - v1
    Checksum:iDD16E1FAA8497AC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83047.1.
    U00096 Genomic DNA. Translation: AAC75904.1.
    AP009048 Genomic DNA. Translation: BAE76932.1.
    PIRiB65070.
    RefSeqiNP_417342.1. NC_000913.3.
    YP_491068.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75904; AAC75904; b2866.
    BAE76932; BAE76932; BAE76932.
    GeneIDi12933325.
    947116.
    KEGGiecj:Y75_p2799.
    eco:b2866.
    PATRICi32121142. VBIEscCol129921_2959.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28375 Genomic DNA. Translation: AAA83047.1 .
    U00096 Genomic DNA. Translation: AAC75904.1 .
    AP009048 Genomic DNA. Translation: BAE76932.1 .
    PIRi B65070.
    RefSeqi NP_417342.1. NC_000913.3.
    YP_491068.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali Q46799.
    SMRi Q46799. Positions 1-746.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q46799. 1 interaction.
    STRINGi 511145.b2866.

    Proteomic databases

    PaxDbi Q46799.
    PRIDEi Q46799.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75904 ; AAC75904 ; b2866 .
    BAE76932 ; BAE76932 ; BAE76932 .
    GeneIDi 12933325.
    947116.
    KEGGi ecj:Y75_p2799.
    eco:b2866.
    PATRICi 32121142. VBIEscCol129921_2959.

    Organism-specific databases

    EchoBASEi EB2861.
    EcoGenei EG13049. xdhA.

    Phylogenomic databases

    eggNOGi COG1529.
    HOGENOMi HOG000244715.
    KOi K00087.
    OMAi AYVSHGH.
    OrthoDBi EOG6ZSP3F.
    PhylomeDBi Q46799.

    Enzyme and pathway databases

    UniPathwayi UPA00604 ; UER00661 .
    UPA00604 ; UER00662 .
    BioCyci EcoCyc:G7485-MONOMER.
    ECOL316407:JW5462-MONOMER.
    MetaCyc:G7485-MONOMER.

    Miscellaneous databases

    PROi Q46799.

    Gene expression databases

    Genevestigatori Q46799.

    Family and domain databases

    Gene3Di 3.30.365.10. 6 hits.
    3.90.1170.50. 1 hit.
    InterProi IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    [Graphical view ]
    Pfami PF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    [Graphical view ]
    SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54665. SSF54665. 1 hit.
    SSF56003. SSF56003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage."
      Xi H., Schneider B.L., Reitzer L.
      J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiXDHA_ECOLI
    AccessioniPrimary (citable) accession number: Q46799
    Secondary accession number(s): Q2M9X4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3