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Q46769

- RMLD_ECOLX

UniProt

Q46769 - RMLD_ECOLX

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Protein
dTDP-4-dehydrorhamnose reductase
Gene
rfbD, rmlD
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well.1 Publication

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Binds 1 magnesium ion per monomer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121NAD; via amide nitrogen By similarity
Binding sitei102 – 1021NADP; via carbonyl oxygen By similarity
Binding sitei155 – 1551Substrate; via amide nitrogen By similarity
Binding sitei156 – 1561NAD; via amide nitrogen By similarity
Binding sitei225 – 2251Substrate By similarity
Binding sitei262 – 2621Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 115NAD By similarity
Nucleotide bindingi11 – 122NADP By similarity
Nucleotide bindingi30 – 312NAD By similarity
Nucleotide bindingi39 – 402NAD/NADP By similarity
Nucleotide bindingi62 – 654NAD By similarity
Nucleotide bindingi63 – 653NADP By similarity
Nucleotide bindingi129 – 1335NAD/NADP By similarity

GO - Molecular functioni

  1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. O antigen biosynthetic process Source: UniProtKB-UniPathway
  2. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  3. extracellular polysaccharide biosynthetic process Source: UniProtKB
  4. lipopolysaccharide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Name:rfbD
Synonyms:rmlD
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301dTDP-4-dehydrorhamnose reductase
PRO_0000207985Add
BLAST

Proteomic databases

PaxDbiQ46769.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ46769.
SMRiQ46769. Positions 1-299.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1052Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1091.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46769-1 [UniParc]FASTAAdd to Basket

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MNILLFGKTG QVGWELQRAL APLGNLIALD VHSTDYCGDF SNPEGVAETV    50
KKIRPDVIVN AAAHTDVDKA ESEPEFAQLL NATSVEAIAK AANEVGAWVI 100
HYSTDYVFPG TGEIPWQGGT DATAPLNVYG ETKLSSEKKA LQKHCGKHII 150
FRTSWVYAGK GNNFAKTMLR LAKEREELAV INDQFGRPTG AELLADCTAH 200
AIRVAVDKPE VAGLYHLVAG GTTTWHDYAA LVFEEARKAG INLALNKLNA 250
VPTTAYPTPA RRPHNSRLNT EKFQQNFALV LPDWQVGVKR MLNELFTTTA 300
I 301
Length:301
Mass (Da):32,866
Last modified:November 1, 1996 - v1
Checksum:iA1C4271C20AB4A13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125322 Genomic DNA. Translation: AAC63613.1.
PIRiS78543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125322 Genomic DNA. Translation: AAC63613.1 .
PIRi S78543.

3D structure databases

ProteinModelPortali Q46769.
SMRi Q46769. Positions 1-299.
ModBasei Search...

Proteomic databases

PaxDbi Q46769.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1091.

Enzyme and pathway databases

UniPathwayi UPA00124 .
UPA00281 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF04321. RmlD_sub_bind. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01214. rmlD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene."
    Marolda C.L., Valvano M.A.
    J. Bacteriol. 177:5539-5546(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS.
    Strain: O7:K1 / VW187.

Entry informationi

Entry nameiRMLD_ECOLX
AccessioniPrimary (citable) accession number: Q46769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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