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Q46769

- RMLD_ECOLX

UniProt

Q46769 - RMLD_ECOLX

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Protein

dTDP-4-dehydrorhamnose reductase

Gene

rfbD

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well.1 Publication

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Binds 1 magnesium ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121NAD; via amide nitrogenBy similarity
Binding sitei102 – 1021NADP; via carbonyl oxygenBy similarity
Binding sitei155 – 1551Substrate; via amide nitrogenBy similarity
Binding sitei156 – 1561NAD; via amide nitrogenBy similarity
Binding sitei225 – 2251SubstrateBy similarity
Binding sitei262 – 2621SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 115NADBy similarity
Nucleotide bindingi11 – 122NADPBy similarity
Nucleotide bindingi30 – 312NADBy similarity
Nucleotide bindingi39 – 402NAD/NADPBy similarity
Nucleotide bindingi62 – 654NADBy similarity
Nucleotide bindingi63 – 653NADPBy similarity
Nucleotide bindingi129 – 1335NAD/NADPBy similarity

GO - Molecular functioni

  1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  2. extracellular polysaccharide biosynthetic process Source: UniProtKB
  3. lipopolysaccharide biosynthetic process Source: UniProtKB
  4. O antigen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Name:rfbD
Synonyms:rmlD
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301dTDP-4-dehydrorhamnose reductasePRO_0000207985Add
BLAST

Proteomic databases

PaxDbiQ46769.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ46769.
SMRiQ46769. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1052Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1091.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46769-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNILLFGKTG QVGWELQRAL APLGNLIALD VHSTDYCGDF SNPEGVAETV
60 70 80 90 100
KKIRPDVIVN AAAHTDVDKA ESEPEFAQLL NATSVEAIAK AANEVGAWVI
110 120 130 140 150
HYSTDYVFPG TGEIPWQGGT DATAPLNVYG ETKLSSEKKA LQKHCGKHII
160 170 180 190 200
FRTSWVYAGK GNNFAKTMLR LAKEREELAV INDQFGRPTG AELLADCTAH
210 220 230 240 250
AIRVAVDKPE VAGLYHLVAG GTTTWHDYAA LVFEEARKAG INLALNKLNA
260 270 280 290 300
VPTTAYPTPA RRPHNSRLNT EKFQQNFALV LPDWQVGVKR MLNELFTTTA

I
Length:301
Mass (Da):32,866
Last modified:November 1, 1996 - v1
Checksum:iA1C4271C20AB4A13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125322 Genomic DNA. Translation: AAC63613.1.
PIRiS78543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125322 Genomic DNA. Translation: AAC63613.1 .
PIRi S78543.

3D structure databases

ProteinModelPortali Q46769.
SMRi Q46769. Positions 1-299.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q46769.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1091.

Enzyme and pathway databases

UniPathwayi UPA00124 .
UPA00281 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR10491. PTHR10491. 1 hit.
TIGRFAMsi TIGR01214. rmlD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene."
    Marolda C.L., Valvano M.A.
    J. Bacteriol. 177:5539-5546(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS.
    Strain: O7:K1 / VW187.

Entry informationi

Entry nameiRMLD_ECOLX
AccessioniPrimary (citable) accession number: Q46769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3