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Protein

AMP phosphorylase

Gene

Mbar_A3188

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.UniRule annotation

Catalytic activityi

AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei167 – 1671AMP; via amide nitrogenUniRule annotation
Binding sitei202 – 2021AMP; via amide nitrogenUniRule annotation
Active sitei255 – 2551Proton donorUniRule annotation
Binding sitei263 – 2631AMPUniRule annotation
Binding sitei287 – 2871AMPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi193 – 1986AMPUniRule annotation

GO - Molecular functioni

  1. AMP binding Source: UniProtKB-HAMAP
  2. phosphorylase activity Source: InterPro
  3. thymidine phosphorylase activity Source: InterPro

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. pyrimidine deoxyribonucleoside metabolic process Source: InterPro
  3. pyrimidine nucleobase metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3243-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AMP phosphorylaseUniRule annotation (EC:2.4.2.57UniRule annotation)
Short name:
AMPpaseUniRule annotation
Alternative name(s):
Nucleoside monophosphate phosphorylaseUniRule annotation
Short name:
NMP phosphorylaseUniRule annotation
Gene namesi
Ordered Locus Names:Mbar_A3188
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506AMP phosphorylasePRO_0000225645Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A3188.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK18931.
OMAiHAKVGYR.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_02132. AMP_phosphorylase.
InterProiIPR017713. AMP_phosphorylase.
IPR009010. Asp_de-COase-like_dom.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF50692. SSF50692. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q466X0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLKLEHFNI KIGQHKILLN IADAKELGVN PGDRVRIRGR ESISAIADTT
60 70 80 90 100
DDMVPPGTLG VFSEVYEHFV NWDKPVEVVP AFRSKSASVI KKMMDKKPVV
110 120 130 140 150
QEEIKTLVND IVEENLSEIE LSAFITSSYI HGMTDDEVEW LTRAMIESGD
160 170 180 190 200
TIEFDTHPIM DKHSIGGVPG NKISLLVVPI IAANGLLIPK TSSRAITGAG
210 220 230 240 250
GTADLMEVLC PVEFSSQEVK EITEKVGGAL VWGGATNIAP ADDKLIRVEY
260 270 280 290 300
PLSIDPYYQM LASIMAKKGA IGADNVVMDI PVGPSTKVPT VQEGQKLARD
310 320 330 340 350
LINLGHRLGM NVECAITYGS SPIGRKVGPS LEVREALKVL ESMEGPNSLI
360 370 380 390 400
EKSAALAGIL LEMGGAAPRD RGKEIALETL RSGKALEKMK QIIEAQGGDP
410 420 430 440 450
KITSADIQVG QYTADILASA DGYVIEFDNK WIIEIARLAG APNDKGAGVA
460 470 480 490 500
IHKKMGESVK KGDPILTIYA EKEFKLETAL ATAQRTNPIV VEGMLLKRIP

GTYGFQ
Length:506
Mass (Da):54,628
Last modified:September 13, 2005 - v1
Checksum:iCA65A31C8EDAE32E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72072.1.
RefSeqiYP_306652.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ72072; AAZ72072; Mbar_A3188.
GeneIDi3627130.
KEGGimba:Mbar_A3188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72072.1.
RefSeqiYP_306652.1. NC_007355.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A3188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ72072; AAZ72072; Mbar_A3188.
GeneIDi3627130.
KEGGimba:Mbar_A3188.

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK18931.
OMAiHAKVGYR.

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3243-MONOMER.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_02132. AMP_phosphorylase.
InterProiIPR017713. AMP_phosphorylase.
IPR009010. Asp_de-COase-like_dom.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF50692. SSF50692. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiAMPPA_METBF
AccessioniPrimary (citable) accession number: Q466X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: September 13, 2005
Last modified: April 1, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.