ID   TRM1_METBF              Reviewed;         388 AA.
AC   Q466T6;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 26.
DE   RecName: Full=N(2),N(2)-dimethylguanosine tRNA methyltransferase;
DE            EC=2.1.1.32;
DE   AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase;
DE   AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase;
DE   AltName: Full=tRNA(m(2,2)G26)dimethyltransferase;
GN   Name=trm1; OrderedLocusNames=Mbar_A3224;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980466; DOI=10.1128/JB.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P.,
RA   Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R.,
RA   Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC       a number of tRNAs using S-adenosyl-L-methionine as donor of the
CC       methyl groups (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(2)-methylguanine.
CC   -!- SIMILARITY: Belongs to the TRM1 family.
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DR   EMBL; CP000099; AAZ72106.1; -; Genomic_DNA.
DR   RefSeq; YP_306686.1; -.
DR   GeneID; 3627341; -.
DR   GenomeReviews; CP000099_GR; Mbar_A3224.
DR   KEGG; mba:Mbar_A3224; -.
DR   NMPDR; fig|269797.3.peg.3383; -.
DR   HOGENOM; Q466T6; -.
DR   OMA; Q466T6; MELNRDI.
DR   BioCyc; MBAR269797:MBAR_A3224-MON; -.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00290; -; 1.
DR   InterPro; IPR002905; TRM_MeTrfase.
DR   PANTHER; PTHR10631; TRM_mtfrase; 1.
DR   Pfam; PF02005; TRM; 1.
DR   TIGRFAMs; TIGR00308; TRM1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    388       N(2),N(2)-dimethylguanosine tRNA
FT                                methyltransferase.
FT                                /FTId=PRO_0000259375.
SQ   SEQUENCE   388 AA;  42848 MW;  6362585D08A3E694 CRC64;
     MIYKTIVEGT TKVSVPVPPP DATFPPSAAP VFYNPEMELN RDINVAATAA FVERLLAKKE
     LLREEVRYVD AFSASGIRGL RIAGEVGIHA TMNDWSPEAF ELIKENSKIN GLEENTLATR
     KSANVLLHEQ KYHIVDIDPF GTPAPFLDAA SASVRGMLSV TATDTAPLCG AHLKAGIRKY
     ASVPLNTEYH SEMGLRVLLG ACARELAKHE KGMLPLLSHV TRHYVRTYLE VLPGTKQTDR
     TLKSMGFSIH CPKCGFRGPV YGLAVHIEKE CPVCGSFTQI AGPLWLGPFR EQAFCDEVIS
     ELEMHPLNTK DKAKKIITFC RDELDIPMFY DQHVICKELG ASATGIEILI EALKAHGFEA
     SRTHFSGTSF RTDAPITEIK EIIRALSW
//