Reviewed, UniProtKB/Swiss-Prot Q466T6 (TRM1_METBF)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	Mbar_A3224

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	N(2),N(2)-dimethylguanosine tRNA methyltransferase HAMAP MF_00290		PRO_0000259375

Sequences

	Sequence		Length	Mass (Da)
	Q466T6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6362585D08A3E694	FASTA	388	42,848
	10 20 30 40 50 60 MIYKTIVEGT TKVSVPVPPP DATFPPSAAP VFYNPEMELN RDINVAATAA FVERLLAKKE 70 80 90 100 110 120 LLREEVRYVD AFSASGIRGL RIAGEVGIHA TMNDWSPEAF ELIKENSKIN GLEENTLATR 130 140 150 160 170 180 KSANVLLHEQ KYHIVDIDPF GTPAPFLDAA SASVRGMLSV TATDTAPLCG AHLKAGIRKY 190 200 210 220 230 240 ASVPLNTEYH SEMGLRVLLG ACARELAKHE KGMLPLLSHV TRHYVRTYLE VLPGTKQTDR 250 260 270 280 290 300 TLKSMGFSIH CPKCGFRGPV YGLAVHIEKE CPVCGSFTQI AGPLWLGPFR EQAFCDEVIS 310 320 330 340 350 360 ELEMHPLNTK DKAKKIITFC RDELDIPMFY DQHVICKELG ASATGIEILI EALKAHGFEA 370 380 SRTHFSGTSF RTDAPITEIK EIIRALSW

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ72106.1.
RefSeq	YP_306686.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3627341.
GenomeReviews	Gene locus Mbar_A3224 in contig CP000099_GR.
KEGG	mba:Mbar_A3224.
NMPDR	fig|269797.3.peg.3383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q466T6.
OMA	Q466T6. MELNRDI.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A3224-MON.
Family and domain databases
HAMAP	MF_00290. [Tree]
InterPro	IPR002905. TRM_MeTrfase. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_METBF

Accession

Primary (citable) accession number: Q466T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)