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Protein

Probable ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei29 – 291S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei31 – 311S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei56 – 561S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei77 – 771S-adenosyl-L-methionineUniRule annotation
Binding sitei102 – 1021S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:Mbar_A3234
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Probable ribosomal RNA small subunit methyltransferase APRO_0000257380Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A3234.

Structurei

3D structure databases

ProteinModelPortaliQ466S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04131. Archaea.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiVKANWYA.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q466S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSIYSGGFS LVRSLLKKYN IKGGTFDQHF LVDAGYLDRI VAAAELGPKD
60 70 80 90 100
VVLEIGAGVG NLTERLAKKV KKVIAIELDP VLVRVLHDRF DKVGNIEIIA
110 120 130 140 150
GDALKVEFPE FDKIVSNLPY SISSEITFKL LRHKFKLGIL MYQYEFAARM
160 170 180 190 200
VSQPNCKDYS RLTVDTCYFA DASILMKVPK SAFQPAPEVD SAVIKLVPRP
210 220 230 240 250
VPFEVKDQAF FMNFVSAVFS QRRKKLRNAI LNTNYLLKIP NIKEVISRLP
260 270 280
EDMMSKRAEN LTPEELAQVA NHIIDLKTSI LANELNE
Length:287
Mass (Da):32,359
Last modified:September 13, 2005 - v1
Checksum:i90FDCCFE7865095F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72116.1.

Genome annotation databases

EnsemblBacteriaiAAZ72116; AAZ72116; Mbar_A3234.
KEGGimba:Mbar_A3234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72116.1.

3D structure databases

ProteinModelPortaliQ466S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A3234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ72116; AAZ72116; Mbar_A3234.
KEGGimba:Mbar_A3234.

Phylogenomic databases

eggNOGiarCOG04131. Archaea.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiVKANWYA.

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3289-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiRSMA_METBF
AccessioniPrimary (citable) accession number: Q466S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 13, 2005
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.