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Q466J4 (THIM_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Mbar_A3319
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383917

Sites

Binding site401Substrate; via amide nitrogen By similarity
Binding site1161ATP By similarity
Binding site1621ATP By similarity
Binding site1891Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q466J4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 3BDFFDC7073D10F5

FASTA26127,903
        10         20         30         40         50         60 
MNDPLKTIRE TKPLIHHITN WVTIYDCANM TRAFGALPVM AHASEECAEM TGISSALVLN 

        70         80         90        100        110        120 
IGTPTSEIID SMLLSAAAAN EKKIPVVLDA VGVGATKFRD EMAAKILGSV RVDIIKGNYS 

       130        140        150        160        170        180 
EIAKLAGESA ETKGVEATSI NADPVKIAKQ FAKLSSSVIV MTGKEDIISD GERVFIVKNG 

       190        200        210        220        230        240 
HELMGSIVGT GCMAASVIGL FAAVNPDYFE ASREALCYFG VAGELAARES NGPGSFKVNL 

       250        260 
YDEVFNLSDE KVRSMMNFEE Q 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ72198.1.
RefSeqYP_306778.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ466J4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A3319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ72198; AAZ72198; Mbar_A3319.
GeneID3626098.
KEGGmba:Mbar_A3319.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-3374-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_METBF
AccessionPrimary (citable) accession number: Q466J4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways