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Q466J4

- THIM_METBF

UniProt

Q466J4 - THIM_METBF

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401Substrate; via amide nitrogenUniRule annotation
    Binding sitei116 – 1161ATPUniRule annotation
    Binding sitei162 – 1621ATPUniRule annotation
    Binding sitei189 – 1891Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-3374-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:Mbar_A3319
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Hydroxyethylthiazole kinasePRO_0000383917Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi269797.Mbar_A3319.

    Structurei

    3D structure databases

    ProteinModelPortaliQ466J4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    HOGENOMiHOG000114352.
    KOiK00878.
    OMAiQTFTANV.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00694. thiM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q466J4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDPLKTIRE TKPLIHHITN WVTIYDCANM TRAFGALPVM AHASEECAEM    50
    TGISSALVLN IGTPTSEIID SMLLSAAAAN EKKIPVVLDA VGVGATKFRD 100
    EMAAKILGSV RVDIIKGNYS EIAKLAGESA ETKGVEATSI NADPVKIAKQ 150
    FAKLSSSVIV MTGKEDIISD GERVFIVKNG HELMGSIVGT GCMAASVIGL 200
    FAAVNPDYFE ASREALCYFG VAGELAARES NGPGSFKVNL YDEVFNLSDE 250
    KVRSMMNFEE Q 261
    Length:261
    Mass (Da):27,903
    Last modified:September 13, 2005 - v1
    Checksum:i3BDFFDC7073D10F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ72198.1.
    RefSeqiWP_011308238.1. NC_007355.1.
    YP_306778.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ72198; AAZ72198; Mbar_A3319.
    GeneIDi3626098.
    KEGGimba:Mbar_A3319.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ72198.1 .
    RefSeqi WP_011308238.1. NC_007355.1.
    YP_306778.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q466J4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A3319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ72198 ; AAZ72198 ; Mbar_A3319 .
    GeneIDi 3626098.
    KEGGi mba:Mbar_A3319.

    Phylogenomic databases

    eggNOGi COG2145.
    HOGENOMi HOG000114352.
    KOi K00878.
    OMAi QTFTANV.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci MBAR269797:GHUW-3374-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00694. thiM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiTHIM_METBF
    AccessioniPrimary (citable) accession number: Q466J4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3