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Q466B1 (FPOH_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
F(420)H(2) oxidoreductase subunit H
Alternative name(s):
F(420)H(2) dehydrogenase subunit H
FPO subunit H
Gene names
Name:fpoH
Ordered Locus Names:Mbar_A3408
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01350

Subunit structure

FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential HAMAP MF_01350.

Sequence similarities

Belongs to the complex I subunit 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandFAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346F(420)H(2) oxidoreductase subunit H HAMAP MF_01350
PRO_0000240122

Regions

Transmembrane18 – 3821Helical; Potential
Transmembrane91 – 11121Helical; Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane170 – 19021Helical; Potential
Transmembrane196 – 21621Helical; Potential
Transmembrane257 – 27721Helical; Potential
Transmembrane284 – 30421Helical; Potential
Transmembrane326 – 34621Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q466B1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: E8F183F644315504

FASTA34638,027
        10         20         30         40         50         60 
MTVVIPEYIT PLIPWVRGIV GLVLIGVIFM GAMGAVWLER KLSADIQTRM GPCRVGKYGL 

        70         80         90        100        110        120 
LQLVADAIKL FTKEDLKPLN ADSLLFNNAN IFMLGSVFLM LVALPVGAVF INGVEYPLAV 

       130        140        150        160        170        180 
TQMDISVLYI EAVSALSIFG IFMVAYGSNN KYSLLGAFRN FARMVGYEVP LGITVISVAA 

       190        200        210        220        230        240 
MTGSLNIVDI STAQGLHWNI FLQPLGCFVF FVSLMADMGR LPFDQNESEE ELIAGWITEY 

       250        260        270        280        290        300 
CGMRFGLGFF AEYIHMILGS FLVALLFLGG WNVPGFIANN SFFGIIVPTG FLIVKVVFVL 

       310        320        330        340 
MVIIGLRWAV PRFRIDQVVD LSWKKLLPLA LLNLVWAVGL GLYLGA 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ72281.1.
RefSeqYP_306861.1. NC_007355.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ466B1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3624655.
GenomeReviewsGene locus Mbar_A3408 in contig CP000099_GR.
KEGGmba:Mbar_A3408.
NMPDRfig|269797.3.peg.1982.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG06916.
HOGENOMHBG727670.
OMAWNIFLQP.
PhylomeDBQ466B1.
ProtClustDBCLSK568843.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A3408-MONOMER.

Family and domain databases

HAMAPMF_01350. NDH1_NuoH.
[Tree]
InterProIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
KOK00337.
PANTHERPTHR11432. Resp_NADH_DH_1. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFPOH_METBF
AccessionPrimary (citable) accession number: Q466B1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: September 13, 2005
Last modified: November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families