Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q466B1 (FPOH_METBF)

Last modified June 16, 2009. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    F(420)H(2) oxidoreductase subunit H
Alternative name(s):
    F(420)H(2) dehydrogenase subunit H
    FPO subunit H
Gene names
Name: fpoH
Ordered Locus Names: Mbar_A3408
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.

Subunit structure

FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the complex I subunit 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   LigandFAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: HAMAP

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346F(420)H(2) oxidoreductase subunit H HAMAP MF_01350
PRO_0000240122

Regions

Transmembrane18 – 3821 Potential
Transmembrane91 – 11121 Potential
Transmembrane125 – 14521 Potential
Transmembrane170 – 19021 Potential
Transmembrane196 – 21621 Potential
Transmembrane257 – 27721 Potential
Transmembrane284 – 30421 Potential
Transmembrane326 – 34621 Potential

Sequences

Sequence LengthMass (Da)Tools
Q466B1-1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: E8F183F644315504

FASTA34638,027
        10         20         30         40         50         60 
MTVVIPEYIT PLIPWVRGIV GLVLIGVIFM GAMGAVWLER KLSADIQTRM GPCRVGKYGL 

        70         80         90        100        110        120 
LQLVADAIKL FTKEDLKPLN ADSLLFNNAN IFMLGSVFLM LVALPVGAVF INGVEYPLAV 

       130        140        150        160        170        180 
TQMDISVLYI EAVSALSIFG IFMVAYGSNN KYSLLGAFRN FARMVGYEVP LGITVISVAA 

       190        200        210        220        230        240 
MTGSLNIVDI STAQGLHWNI FLQPLGCFVF FVSLMADMGR LPFDQNESEE ELIAGWITEY 

       250        260        270        280        290        300 
CGMRFGLGFF AEYIHMILGS FLVALLFLGG WNVPGFIANN SFFGIIVPTG FLIVKVVFVL 

       310        320        330        340 
MVIIGLRWAV PRFRIDQVVD LSWKKLLPLA LLNLVWAVGL GLYLGA 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000099 Genomic DNA. Translation: AAZ72281.1.
RefSeqYP_306861.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3624655.
GenomeReviewsGene locus Mbar_A3408 in contig CP000099_GR.
KEGGmba:Mbar_A3408.
NMPDRfig|269797.3.peg.1982.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ466B1.
OMAQ466B1. LYLGGWE.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A3408-MON.

Family and domain databases

HAMAPMF_01350.
[Tree]
InterProIPR001694. NADH_UbQ_OxRdtase_su1.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERPTHR11432. Resp_NADH_DH_1. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFPOH_METBF
AccessionPrimary (citable) accession number: Q466B1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: September 13, 2005
Last modified: June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents