Reviewed, UniProtKB/Swiss-Prot Q466B1 (FPOH_METBF)

Names and origin

Protein names

Recommended name:
    F(420)H(2) oxidoreductase subunit H
Alternative name(s):
    F(420)H(2) dehydrogenase subunit H
    FPO subunit H

Gene names

Name:	fpoH
Ordered Locus Names:	Mbar_A3408

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Subunit structure	FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	FAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	F(420)H(2) oxidoreductase subunit H HAMAP MF_01350		PRO_0000240122
Regions
	Transmembrane	18 – 38	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	125 – 145	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	196 – 216	21	Potential
	Transmembrane	257 – 277	21	Potential
	Transmembrane	284 – 304	21	Potential
	Transmembrane	326 – 346	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q466B1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E8F183F644315504	FASTA	346	38,027
	10 20 30 40 50 60 MTVVIPEYIT PLIPWVRGIV GLVLIGVIFM GAMGAVWLER KLSADIQTRM GPCRVGKYGL 70 80 90 100 110 120 LQLVADAIKL FTKEDLKPLN ADSLLFNNAN IFMLGSVFLM LVALPVGAVF INGVEYPLAV 130 140 150 160 170 180 TQMDISVLYI EAVSALSIFG IFMVAYGSNN KYSLLGAFRN FARMVGYEVP LGITVISVAA 190 200 210 220 230 240 MTGSLNIVDI STAQGLHWNI FLQPLGCFVF FVSLMADMGR LPFDQNESEE ELIAGWITEY 250 260 270 280 290 300 CGMRFGLGFF AEYIHMILGS FLVALLFLGG WNVPGFIANN SFFGIIVPTG FLIVKVVFVL 310 320 330 340 MVIIGLRWAV PRFRIDQVVD LSWKKLLPLA LLNLVWAVGL GLYLGA

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ72281.1.
RefSeq	YP_306861.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3624655.
GenomeReviews	Gene locus Mbar_A3408 in contig CP000099_GR.
KEGG	mba:Mbar_A3408.
NMPDR	fig|269797.3.peg.1982.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q466B1.
OMA	Q466B1. LYLGGWE.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A3408-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPOH_METBF

Accession

Primary (citable) accession number: Q466B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)