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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.UniRule annotation

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.UniRule annotation

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3473-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferaseUniRule annotation (EC:2.7.7.68UniRule annotation)
Short name:
LP guanylyltransferaseUniRule annotation
Gene namesi
Name:cofCUniRule annotation
Ordered Locus Names:Mbar_A3418
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2082082-phospho-L-lactate guanylyltransferasePRO_0000398756Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A3418.

Structurei

3D structure databases

ProteinModelPortaliQ466A1.
SMRiQ466A1. Positions 1-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q466A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVIPYKKS SAKSRLSPVL TREEREEFVD LMLNQVIDTL KEAGVGTIDI
60 70 80 90 100
LSPSMYGLEN MTKANVLLDK NDLNEALNGY LEQAEEPVII VMADLPLLSP
110 120 130 140 150
DHVKGITSTK EDVCIVPGKG GGTNALFIKN PSCYRVRYYG SSFLTHCSIA
160 170 180 190 200
EKTGQSVEVY DSFFAGTDID EPEDLVELLI HGSGAAKEYI SKKFRLEMSR

GRVGLVHI
Length:208
Mass (Da):22,916
Last modified:September 13, 2005 - v1
Checksum:i4AE443D9E6BE9B94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72291.1.
RefSeqiWP_011308330.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ72291; AAZ72291; Mbar_A3418.
GeneIDi3624705.
KEGGimba:Mbar_A3418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ72291.1.
RefSeqiWP_011308330.1. NC_007355.1.

3D structure databases

ProteinModelPortaliQ466A1.
SMRiQ466A1. Positions 1-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A3418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ72291; AAZ72291; Mbar_A3418.
GeneIDi3624705.
KEGGimba:Mbar_A3418.

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMBAR269797:GHUW-3473-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiCOFC_METBF
AccessioniPrimary (citable) accession number: Q466A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 13, 2005
Last modified: December 9, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.