Reviewed, UniProtKB/Swiss-Prot Q46684 (BGLX_ERWCH)

Names and origin

Protein names

Recommended name:
    Periplasmic beta-glucosidase/beta-xylosidase
Including the following 2 domains:
    1- Recommended name:
            Beta-glucosidase
              EC=3.2.1.21
        Alternative name(s):
            Gentiobiase
            Cellobiase
    2- Recommended name:
            Beta-xylosidase
              EC=3.2.1.37
        Alternative name(s):
            1,4-beta-D-xylan xylohydrolase
            Xylan 1,4-beta-xylosidase

Gene names

Name:

bgxA

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits both beta-glucosidase and beta-xylosidase activities.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC xylan 1,4-beta-xylosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 654	629	Periplasmic beta-glucosidase/beta-xylosidase		PRO_0000011783
Sites
	Active site	235	1	By similarity
	Active site	360	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46684-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5CEDFE62162A7A95	FASTA	654	71,584
	10 20 30 40 50 60 MEKSATRQKA LLIALPLLFS PLASAVQQAV LDTRGAPLIT VNGLTFKDLN RDGKLNPYED 70 80 90 100 110 120 WRLPAAERAA DLVSRMTLAE KAGVMMHGSA PTAGSVTGAG TQYDLNAAKT MIADRYVNSF 130 140 150 160 170 180 ITRLSGDNPA QMAEENNKLQ QLAEATRLGI PLTISTDPRS SFQSLVGVSV SVGKFSKWPE 190 200 210 220 230 240 TLGLAAIGDE ELVRRFADIV RQEYRAVGIT EALSPQADLA TEPRWPRIDG TFGEDPDLTK 250 260 270 280 290 300 KMVRGYVTGM QNGKNGLNAQ SVISIVKHWV GYGAAKDGWD SHNVYGKYAQ FRQNNLQWHI 310 320 330 340 350 360 DPFTGAFEAH AAGIMPTYSI LRNASWHGKP IEQVGAGFNR FLLTDLLRGQ YGFDGVILSD 370 380 390 400 410 420 WLITNDCKGD CLTGVKPGEK PVPRGMPWGV EKLTPAERFV KAVNAGVDQF GGVTDSALLV 430 440 450 460 470 480 QAVQDGKLTE ARLDTSVNRI LKQKFQTGLF ERPYVNATQA NDIVGRADWQ QLADDTQARS 490 500 510 520 530 540 LVLLQNNNLL PLRKGSRVWL HGIAANAAQE VGFIVVNTPE QADVALIRTH TPYEQPHKNF 550 560 570 580 590 600 FFGSRHHEGS LAFRNDNPDY QAIVRASAKV PTLVTVYMER PAILTNVVDK TRAVVANFGV 610 620 630 640 650 SDSVLLNRLM SGAAYTAKLP FELPSSMSAV RNQQPDLPYD SAKPLFPFGY GLPH

References

[1]

"Cloning and characterization of the bgxA gene from Erwinia chrysanthemi D1 which encodes a beta-glucosidase/xylosidase enzyme." Vroemen S., Heldens J., Boyd C., Henrissat B., Keen N.T. Mol. Gen. Genet. 246:465-477(1995) [PubMed: 7891660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08606 Genomic DNA. Translation: AAA80156.1.
PIR	S53805.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Enzyme and pathway databases
BRENDA	3.2.1.21. 1459. 3.2.1.37. 1459.
Family and domain databases
InterPro	IPR019800. Glyco_hydro_3_AS. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 2 hits. PF01915. Glyco_hydro_3_C. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLX_ERWCH

Accession

Primary (citable) accession number: Q46684

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)