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Protein

Probable low molecular weight protein-tyrosine-phosphatase AmsI

Gene

amsI

Organism
Erwinia amylovora (Fire blight bacteria)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91NucleophileBy similarity
Active sitei14 – 141By similarity
Active sitei115 – 1151Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Exopolysaccharide synthesis, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Probable low molecular weight protein-tyrosine-phosphatase AmsI (EC:3.1.3.48)
Gene namesi
Name:amsI
OrganismiErwinia amylovora (Fire blight bacteria)
Taxonomic identifieri552 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Probable low molecular weight protein-tyrosine-phosphatase AmsIPRO_0000046578Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi665029.EAMY_2251.

Structurei

Secondary structure

1
144
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1411Combined sources
Helixi15 – 2612Combined sources
Beta strandi30 – 367Combined sources
Helixi47 – 559Combined sources
Helixi70 – 756Combined sources
Beta strandi77 – 837Combined sources
Helixi85 – 939Combined sources
Helixi95 – 1006Combined sources
Beta strandi101 – 1033Combined sources
Helixi121 – 14323Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4D74X-ray1.57A1-144[»]
ProteinModelPortaliQ46630.
SMRiQ46630. Positions 1-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107ZF2. Bacteria.
COG0394. LUCA.

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF20. PTHR11717:SF20. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINSILVVCI GNICRSPTGE RLLKAALPER KIASAGLKAM VGGSADETAS
60 70 80 90 100
IVANEHGVSL QDHVAQQLTA DMCRDSDLIL VMEKKHIDLV CRINPSVRGK
110 120 130 140
TMLFGHWINQ QEIADPYKKS RDAFEAVYGV LENAAQKWVN ALSR
Length:144
Mass (Da):15,772
Last modified:November 1, 1997 - v1
Checksum:i85B64E5EBC52961F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77921 Genomic DNA. Translation: CAA54881.1.
PIRiS61893. S52140.

Genome annotation databases

GeneIDi8912405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77921 Genomic DNA. Translation: CAA54881.1.
PIRiS61893. S52140.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4D74X-ray1.57A1-144[»]
ProteinModelPortaliQ46630.
SMRiQ46630. Positions 1-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi665029.EAMY_2251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8912405.

Phylogenomic databases

eggNOGiENOG4107ZF2. Bacteria.
COG0394. LUCA.

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF20. PTHR11717:SF20. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora."
    Bugert P., Geider K.
    Mol. Microbiol. 15:917-933(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EA1/79.

Entry informationi

Entry nameiAMSI_ERWAM
AccessioniPrimary (citable) accession number: Q46630
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 13, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.