Reviewed, UniProtKB/Swiss-Prot Q46630 (AMSI_ERWAM)

Names and origin

Protein names

Recommended name:
    Probable low molecular weight protein-tyrosine-phosphatase amsI
    EC=3.1.3.48

Gene names

Name:

amsI

Organism

Erwinia amylovora (Fire blight bacteria)

Taxonomic identifier

552 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis Virulence
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Probable low molecular weight protein-tyrosine-phosphatase amsI		PRO_0000046578
Sites
	Active site	9	1	Nucleophile By similarity
	Active site	14	1	By similarity
	Active site	115	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46630-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 85B64E5EBC52961F	FASTA	144	15,772
	10 20 30 40 50 60 MINSILVVCI GNICRSPTGE RLLKAALPER KIASAGLKAM VGGSADETAS IVANEHGVSL 70 80 90 100 110 120 QDHVAQQLTA DMCRDSDLIL VMEKKHIDLV CRINPSVRGK TMLFGHWINQ QEIADPYKKS 130 140 RDAFEAVYGV LENAAQKWVN ALSR

References

[1]	"Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora." Bugert P., Geider K. Mol. Microbiol. 15:917-933(1995) [PubMed: 7596293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EA1/79.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77921 Genomic DNA. Translation: CAA54881.1.
PIR	S52140. S61893.
3D structure databases
SMR	Q46630. Positions 1-144.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.48. 81763.
Family and domain databases
InterPro	IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMSI_ERWAM

Accession

Primary (citable) accession number: Q46630

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)