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Q465S3 (Q465S3_METBF) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Ordered Locus Names:Mbar_A3498
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] EMBL AAZ72369.1
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site51For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3921Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4431Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q465S3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: C7BBACBF596A0066

FASTA47752,340
        10         20         30         40         50         60 
MKEECGVAGI ILPGDRPQSN TVAFKLYYAL YALQHRGQES TGIMVYDGTS SHSIKGMGLV 

        70         80         90        100        110        120 
PDVYNKDSLG HLVGNVGVGH VRYSTTGGSK IENCQPFVLK FKGGAVAIAH NGNLVNARTL 

       130        140        150        160        170        180 
KDELECEGRI FISESDTEVI GHLLVKELIR HDPIESIRNV MRKLVGSYSL VIHIGGVLYA 

       190        200        210        220        230        240 
VRDPLGLKPL CFGEVDGGYA VFSESVALDT LNGTLIRDVR PGEVIVFTGD SFESYQIGNE 

       250        260        270        280        290        300 
PHPAHCVFEF IYFARPDSII DGKLVYKVRE NIGRELAREH PVEADVVSPV PDSGITSAIG 

       310        320        330        340        350        360 
YARESGTKYL EGLMKNRYIG RTFILPGQEL RETAVRLKMN AIQDNVKDKR VVLVDDSIVR 

       370        380        390        400        410        420 
GTTSRRIIDM VRRAGASEIH ARIGSPAIIA PCYLGIDMAT RQELIASYKT VKEVEGLINA 

       430        440        450        460        470 
DSLGYLSIDG LMRALGRDKC DMCLGCLTGE YPVEIPGENC IRKQTRLDDF NNNQESS

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References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000099 Genomic DNA. Translation: AAZ72369.1.
RefSeqYP_306949.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ465S3.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ465S3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3624896.
GenomeReviewsGene locus Mbar_A3498 in contig CP000099_GR.
KEGGmba:Mbar_A3498.
NMPDRfig|269797.3.peg.2353.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04070.
HOGENOMHBG392416.
OMAVWAPGEE.
PhylomeDBQ465S3.
ProtClustDBCLSK811961.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ465S3_METBF
AccessionPrimary (citable) accession number: Q465S3
Entry history
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info