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Q465Q9 (PUR2_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Mbar_A3513
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 433433Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018821

Regions

Domain110 – 317208ATP-grasp
Nucleotide binding137 – 19458ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 2 By similarity
Metal binding2891Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q465Q9 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: FE1CFE41937107CC

FASTA43346,905
        10         20         30         40         50         60 
MKILLIGGGG REHAIAEGIK KSKHNPSLYA LMAKKNPGIA ALCEDFLLEK ETEVEKVVEY 

        70         80         90        100        110        120 
AKARNIEMAF VGPEAPLAAG VADALWEAGI PVVGPKKSCA IIEFDKAWAR NFMKKYGIEG 

       130        140        150        160        170        180 
CPEYEVFTEE KPAHDFIEKL GDVAVKPSGL TGGKGVKVMG DQLPDLKAAK AYTSELLEKG 

       190        200        210        220        230        240 
SVVIEERFIG EEFTLQAFVD GKSLVFFPAV QDHKRAYEGD LGPNTGGMGS YTDAGEILPF 

       250        260        270        280        290        300 
MLPEDLEKAK KIMQDTVKAL SEETGIGYQG ILYGQFILTA SGPKVVEFNA RFGDPEAMNV 

       310        320        330        340        350        360 
ISLLETDFVD IMSAVVKGTL GNLPVSFSKK ATVCKYAVPA GYPENPEKDS EVTVEDIGDA 

       370        380        390        400        410        420 
SIYYASVYEK EGKVYTTSSR AIAVIGIAET IAAAEKIAQN ALENLHGKLF FRKDIGTAAL 

       430 
IQKRIDHMKE LRG 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ72383.1.
RefSeqYP_306963.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ465Q9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A3513.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ72383; AAZ72383; Mbar_A3513.
GeneID3625401.
KEGGmba:Mbar_A3513.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-3570-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METBF
AccessionPrimary (citable) accession number: Q465Q9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways