Q465Q9 (PUR2_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Mbar_A3513

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina barkeri

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_1000018821
Regions
	Domain	110 – 317	208	ATP-grasp
	Nucleotide binding	137 – 194	58	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese 1 By similarity
	Metal binding	287	1	Magnesium or manganese 1 By similarity
	Metal binding	287	1	Magnesium or manganese 2 By similarity
	Metal binding	289	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q465Q9 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: FE1CFE41937107CC	FASTA	433	46,905
	10 20 30 40 50 60 MKILLIGGGG REHAIAEGIK KSKHNPSLYA LMAKKNPGIA ALCEDFLLEK ETEVEKVVEY 70 80 90 100 110 120 AKARNIEMAF VGPEAPLAAG VADALWEAGI PVVGPKKSCA IIEFDKAWAR NFMKKYGIEG 130 140 150 160 170 180 CPEYEVFTEE KPAHDFIEKL GDVAVKPSGL TGGKGVKVMG DQLPDLKAAK AYTSELLEKG 190 200 210 220 230 240 SVVIEERFIG EEFTLQAFVD GKSLVFFPAV QDHKRAYEGD LGPNTGGMGS YTDAGEILPF 250 260 270 280 290 300 MLPEDLEKAK KIMQDTVKAL SEETGIGYQG ILYGQFILTA SGPKVVEFNA RFGDPEAMNV 310 320 330 340 350 360 ISLLETDFVD IMSAVVKGTL GNLPVSFSKK ATVCKYAVPA GYPENPEKDS EVTVEDIGDA 370 380 390 400 410 420 SIYYASVYEK EGKVYTTSSR AIAVIGIAET IAAAEKIAQN ALENLHGKLF FRKDIGTAAL 430 IQKRIDHMKE LRG

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ72383.1.
RefSeq	YP_306963.1. NC_007355.1.
3D structure databases
HSSP	HSSP built from PDB template 1VKZ based on UniProtKB Q9X0X7.
ProteinModelPortal	Q465Q9.
ModBase	Search...
Protein-protein interaction databases
STRING	269797.Mbar_A3513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ72383; AAZ72383; Mbar_A3513.
GeneID	3625401.
KEGG	mba:Mbar_A3513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033464.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	MBAR269797:GHUW-3583-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METBF

Accession

Primary (citable) accession number: Q465Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program