Reviewed, UniProtKB/Swiss-Prot Q465K9 (G3P2_METBF)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 2
      Short name=GAPDH 2
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase 2

Gene names

Name:	gap2
Ordered Locus Names:	Mbar_A3564

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Glyceraldehyde-3-phosphate dehydrogenase 2 HAMAP MF_00559		PRO_0000232391
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	300	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q465K9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: B1610F59B57B7710	FASTA	338	36,868
	10 20 30 40 50 60 MAKAKIAVNG YGTIGKRVAD AVRAQDDMEV VGISKTKPNY EAAVAHRLGY DIYAPAANLE 70 80 90 100 110 120 AFEKAGMPAA GSIEEMLEKA DLVVDCTPGG IGEKNKPIYE KVGIKAIWQG GESHPIAGFS 130 140 150 160 170 180 FNAESNYEQA VGRDLTRVVS CNTTALCRAI STIDRELGVN KVRASLSRRA VDPNEIKKGP 190 200 210 220 230 240 VDAIVLNPVK LPSHHGPDVR SVLPHINITT AAIKVPTTLM HVHTVNMEVN KDCTAEDVKN 250 260 270 280 290 300 IFGSQSRIRL VGQGITSTAE IIEFARDIGR PRHDMWELCI WPESITVTDK ELYFFHAVHQ 310 320 330 ESIVVPENVD AIRAMMELES DGAKSIEKTN KAIGLYNK

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ72433.1.
RefSeq	YP_307013.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3626493.
GenomeReviews	Gene locus Mbar_A3564 in contig CP000099_GR.
KEGG	mba:Mbar_A3564.
NMPDR	fig|269797.3.peg.2568.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q465K9.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A3564-MON.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006436. Glyceraldehyde-3-P_DHase_2_arc. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P2_METBF

Accession

Primary (citable) accession number: Q465K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)