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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84NucleophileUniRule annotation1
Binding sitei115L-glutamineUniRule annotation1
Active sitei179Charge relay systemUniRule annotation1
Active sitei181Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:Mbar_A3580
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002558221 – 200Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST200

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A3580

Structurei

3D structure databases

ProteinModelPortaliQ465J4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54L-glutamine bindingUniRule annotation3
Regioni143 – 144L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034 Archaea
COG0311 LUCA
HOGENOMiHOG000039949
KOiK08681
OMAiVFIRAPI
OrthoDBiPOG093Z0AY8

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q465J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGVIAIQG AVSEHIDALR RALKERGMSA EVVAVKHKGV IPECGGIVIP
60 70 80 90 100
GGESTTLCRL LAREGIAEEI KDASARGVPI LGTCAGLIVL SKEGDEQVEK
110 120 130 140 150
TSQELLGIMD TKVNRNAFGR QRDSFEAELE VEILDSPFTG VFIRAPGIVS
160 170 180 190 200
CGPEVRVLSR LDDLIIAAEQ GNVLALAFHP ELTEDLRIHQ YFLDKIFKNA
Length:200
Mass (Da):21,628
Last modified:September 13, 2005 - v1
Checksum:i08EA75C8B1F731DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA Translation: AAZ72448.1
RefSeqiWP_011308487.1, NC_007355.1

Genome annotation databases

EnsemblBacteriaiAAZ72448; AAZ72448; Mbar_A3580
GeneIDi3625804
KEGGimba:Mbar_A3580

Similar proteinsi

Entry informationi

Entry nameiPDXT_METBF
AccessioniPrimary (citable) accession number: Q465J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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