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Q465G4

- SYI_METBF

UniProt

Q465G4 - SYI_METBF

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mbar_A3613
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Isoleucine--tRNA ligasePRO_1000022152Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A3613.

Structurei

3D structure databases

ProteinModelPortaliQ465G4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi596 – 6005"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q465G4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKEITAKYN AEQIEKKITQ LWKDSDAYRK TREHRKTGKR LFFVDGPPYT
60 70 80 90 100
TGHIHLGTAW NKIIKDSILR YYSMNNRNIL ERPGWDMHGL PIEVKVEGVL
110 120 130 140 150
GFKSKKDIES FGVENFIEKC KEFAINQKQE MTEQFQRLGV WMQWEAPYMT
160 170 180 190 200
LKDDYIEAAW WTLKQASEKN LLDVGKRSVN WCPRCETAIA DSEVEYAERT
210 220 230 240 250
DPSIYVKFKI KGEENTFIVI WTTTPWTIPA NVAVAVHPGF EYSKFRAIRQ
260 270 280 290 300
DGSDEILIAA TDLIENVLRQ GRYVDYEVLE TMLGEDLTKL EYESPVGDLV
310 320 330 340 350
PVQNEIKHGI YLADYVTAEN TGCVHIAPGH GMDDFNVGVK YNLPILCPVG
360 370 380 390 400
PNGAYTEEAG EYAGKNVREA NPIVIEDLRK RNRLLAEGTV THRYGHCWRC
410 420 430 440 450
KTPIIYLATE QWFLKVTDIK DKMLEAIDAV DWYPEWAGSA RFRTWVEGAR
460 470 480 490 500
DWCISRQRYW GMPIPVWKCK KCGKLEVIGT KAELLEKSGA GSDVELHRPY
510 520 530 540 550
VDKLTIPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT REQFDEWWPA
560 570 580 590 600
DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS
610 620 630 640 650
LGNVVSPIDV IDKYGADTLR AYVLSSSAPW DDLKFNQEEV ENVHRSINIL
660 670 680 690 700
WNVFRFPLPY MALDNFDPLK VSLDSVKDAL REEDRWILSR IQSVVKAVDE
710 720 730 740 750
AMSGYFLHKA VREILEFTLE DLSRWYIQLI RPRTWTEADD PDKLAAYRVL
760 770 780 790 800
YEVYVTLTKL ISPFMPYLAE EMYQNLIRNV DPNALESVHM CDWPKVNEAY
810 820 830 840 850
LDPELEAAMS TARSIVEAAS NARQKAGRKL RWPVSRIVVS PESEDAAKAV
860 870 880 890 900
ERLRSVLMDQ TNSKAIVLTP VGESWDELGL EVIPDPSKIG PVFKKDAGKV
910 920 930 940 950
IPALQKVDGF ALKKAFAEAG EFELSLADRT TVTVTPGMAN FKETLPEGTA
960 970 980 990 1000
SAESDAGLVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIRVS
1010 1020 1030 1040 1050
VRIEDERVLR LVETLKDLIA EEVRAEILNL GSDIDVSGAL VKDWDVEGIA

MKMGISKK
Length:1,058
Mass (Da):120,510
Last modified:September 13, 2005 - v1
Checksum:i81BE7252A232D33A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ72478.1.
RefSeqiWP_011308517.1. NC_007355.1.
YP_307058.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ72478; AAZ72478; Mbar_A3613.
GeneIDi3626315.
KEGGimba:Mbar_A3613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ72478.1 .
RefSeqi WP_011308517.1. NC_007355.1.
YP_307058.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q465G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A3613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ72478 ; AAZ72478 ; Mbar_A3613 .
GeneIDi 3626315.
KEGGi mba:Mbar_A3613.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MBAR269797:GHUW-3678-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiSYI_METBF
AccessioniPrimary (citable) accession number: Q465G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3