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Q465G4

- SYI_METBF

UniProt

Q465G4 - SYI_METBF

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei599 – 5991ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-3678-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mbar_A3613
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10581058Isoleucine--tRNA ligasePRO_1000022152Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi269797.Mbar_A3613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ465G4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q465G4-1 [UniParc]FASTAAdd to Basket

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    MIKEITAKYN AEQIEKKITQ LWKDSDAYRK TREHRKTGKR LFFVDGPPYT     50
    TGHIHLGTAW NKIIKDSILR YYSMNNRNIL ERPGWDMHGL PIEVKVEGVL 100
    GFKSKKDIES FGVENFIEKC KEFAINQKQE MTEQFQRLGV WMQWEAPYMT 150
    LKDDYIEAAW WTLKQASEKN LLDVGKRSVN WCPRCETAIA DSEVEYAERT 200
    DPSIYVKFKI KGEENTFIVI WTTTPWTIPA NVAVAVHPGF EYSKFRAIRQ 250
    DGSDEILIAA TDLIENVLRQ GRYVDYEVLE TMLGEDLTKL EYESPVGDLV 300
    PVQNEIKHGI YLADYVTAEN TGCVHIAPGH GMDDFNVGVK YNLPILCPVG 350
    PNGAYTEEAG EYAGKNVREA NPIVIEDLRK RNRLLAEGTV THRYGHCWRC 400
    KTPIIYLATE QWFLKVTDIK DKMLEAIDAV DWYPEWAGSA RFRTWVEGAR 450
    DWCISRQRYW GMPIPVWKCK KCGKLEVIGT KAELLEKSGA GSDVELHRPY 500
    VDKLTIPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT REQFDEWWPA 550
    DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS 600
    LGNVVSPIDV IDKYGADTLR AYVLSSSAPW DDLKFNQEEV ENVHRSINIL 650
    WNVFRFPLPY MALDNFDPLK VSLDSVKDAL REEDRWILSR IQSVVKAVDE 700
    AMSGYFLHKA VREILEFTLE DLSRWYIQLI RPRTWTEADD PDKLAAYRVL 750
    YEVYVTLTKL ISPFMPYLAE EMYQNLIRNV DPNALESVHM CDWPKVNEAY 800
    LDPELEAAMS TARSIVEAAS NARQKAGRKL RWPVSRIVVS PESEDAAKAV 850
    ERLRSVLMDQ TNSKAIVLTP VGESWDELGL EVIPDPSKIG PVFKKDAGKV 900
    IPALQKVDGF ALKKAFAEAG EFELSLADRT TVTVTPGMAN FKETLPEGTA 950
    SAESDAGLVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIRVS 1000
    VRIEDERVLR LVETLKDLIA EEVRAEILNL GSDIDVSGAL VKDWDVEGIA 1050
    MKMGISKK 1058
    Length:1,058
    Mass (Da):120,510
    Last modified:September 13, 2005 - v1
    Checksum:i81BE7252A232D33A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ72478.1.
    RefSeqiYP_307058.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ72478; AAZ72478; Mbar_A3613.
    GeneIDi3626315.
    KEGGimba:Mbar_A3613.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ72478.1 .
    RefSeqi YP_307058.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q465G4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A3613.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ72478 ; AAZ72478 ; Mbar_A3613 .
    GeneIDi 3626315.
    KEGGi mba:Mbar_A3613.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-3678-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiSYI_METBF
    AccessioniPrimary (citable) accession number: Q465G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3