Q465G4 (SYI_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	Mbar_A3613

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina barkeri

Protein attributes

Sequence length	1058 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1058	1058	Isoleucine--tRNA ligase HAMAP-Rule MF_02003		PRO_1000022152
Regions
	Motif	48 – 58	11	"HIGH" region HAMAP-Rule MF_02003
	Motif	596 – 600	5	"KMSKS" region HAMAP-Rule MF_02003
Sites
	Binding site	599	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q465G4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 81BE7252A232D33A	FASTA	1,058	120,510
	10 20 30 40 50 60 MIKEITAKYN AEQIEKKITQ LWKDSDAYRK TREHRKTGKR LFFVDGPPYT TGHIHLGTAW 70 80 90 100 110 120 NKIIKDSILR YYSMNNRNIL ERPGWDMHGL PIEVKVEGVL GFKSKKDIES FGVENFIEKC 130 140 150 160 170 180 KEFAINQKQE MTEQFQRLGV WMQWEAPYMT LKDDYIEAAW WTLKQASEKN LLDVGKRSVN 190 200 210 220 230 240 WCPRCETAIA DSEVEYAERT DPSIYVKFKI KGEENTFIVI WTTTPWTIPA NVAVAVHPGF 250 260 270 280 290 300 EYSKFRAIRQ DGSDEILIAA TDLIENVLRQ GRYVDYEVLE TMLGEDLTKL EYESPVGDLV 310 320 330 340 350 360 PVQNEIKHGI YLADYVTAEN TGCVHIAPGH GMDDFNVGVK YNLPILCPVG PNGAYTEEAG 370 380 390 400 410 420 EYAGKNVREA NPIVIEDLRK RNRLLAEGTV THRYGHCWRC KTPIIYLATE QWFLKVTDIK 430 440 450 460 470 480 DKMLEAIDAV DWYPEWAGSA RFRTWVEGAR DWCISRQRYW GMPIPVWKCK KCGKLEVIGT 490 500 510 520 530 540 KAELLEKSGA GSDVELHRPY VDKLTIPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT 550 560 570 580 590 600 REQFDEWWPA DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS 610 620 630 640 650 660 LGNVVSPIDV IDKYGADTLR AYVLSSSAPW DDLKFNQEEV ENVHRSINIL WNVFRFPLPY 670 680 690 700 710 720 MALDNFDPLK VSLDSVKDAL REEDRWILSR IQSVVKAVDE AMSGYFLHKA VREILEFTLE 730 740 750 760 770 780 DLSRWYIQLI RPRTWTEADD PDKLAAYRVL YEVYVTLTKL ISPFMPYLAE EMYQNLIRNV 790 800 810 820 830 840 DPNALESVHM CDWPKVNEAY LDPELEAAMS TARSIVEAAS NARQKAGRKL RWPVSRIVVS 850 860 870 880 890 900 PESEDAAKAV ERLRSVLMDQ TNSKAIVLTP VGESWDELGL EVIPDPSKIG PVFKKDAGKV 910 920 930 940 950 960 IPALQKVDGF ALKKAFAEAG EFELSLADRT TVTVTPGMAN FKETLPEGTA SAESDAGLVY 970 980 990 1000 1010 1020 VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIRVS VRIEDERVLR LVETLKDLIA 1030 1040 1050 EEVRAEILNL GSDIDVSGAL VKDWDVEGIA MKMGISKK

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ72478.1.
RefSeq	YP_307058.1. NC_007355.1.
3D structure databases
HSSP	HSSP built from PDB template 1WNY based on UniProtKB P56690.
ProteinModelPortal	Q465G4.
ModBase	Search...
Protein-protein interaction databases
STRING	269797.Mbar_A3613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ72478; AAZ72478; Mbar_A3613.
GeneID	3626315.
KEGG	mba:Mbar_A3613.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246403.
KO	K01870.
OMA	WFYSQLG.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	MBAR269797:GHUW-3683-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02003. Ile_tRNA_synth_type2.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_METBF

Accession

Primary (citable) accession number: Q465G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program