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Q465G4

- SYI_METBF

UniProt

Q465G4 - SYI_METBF

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Mbar_A3613
Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-3678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Mbar_A3613
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Isoleucine--tRNA ligaseUniRule annotationPRO_1000022152Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A3613.

Structurei

3D structure databases

ProteinModelPortaliQ465G4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotationAdd
BLAST
Motifi596 – 6005"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q465G4-1 [UniParc]FASTAAdd to Basket

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MIKEITAKYN AEQIEKKITQ LWKDSDAYRK TREHRKTGKR LFFVDGPPYT     50
TGHIHLGTAW NKIIKDSILR YYSMNNRNIL ERPGWDMHGL PIEVKVEGVL 100
GFKSKKDIES FGVENFIEKC KEFAINQKQE MTEQFQRLGV WMQWEAPYMT 150
LKDDYIEAAW WTLKQASEKN LLDVGKRSVN WCPRCETAIA DSEVEYAERT 200
DPSIYVKFKI KGEENTFIVI WTTTPWTIPA NVAVAVHPGF EYSKFRAIRQ 250
DGSDEILIAA TDLIENVLRQ GRYVDYEVLE TMLGEDLTKL EYESPVGDLV 300
PVQNEIKHGI YLADYVTAEN TGCVHIAPGH GMDDFNVGVK YNLPILCPVG 350
PNGAYTEEAG EYAGKNVREA NPIVIEDLRK RNRLLAEGTV THRYGHCWRC 400
KTPIIYLATE QWFLKVTDIK DKMLEAIDAV DWYPEWAGSA RFRTWVEGAR 450
DWCISRQRYW GMPIPVWKCK KCGKLEVIGT KAELLEKSGA GSDVELHRPY 500
VDKLTIPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT REQFDEWWPA 550
DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS 600
LGNVVSPIDV IDKYGADTLR AYVLSSSAPW DDLKFNQEEV ENVHRSINIL 650
WNVFRFPLPY MALDNFDPLK VSLDSVKDAL REEDRWILSR IQSVVKAVDE 700
AMSGYFLHKA VREILEFTLE DLSRWYIQLI RPRTWTEADD PDKLAAYRVL 750
YEVYVTLTKL ISPFMPYLAE EMYQNLIRNV DPNALESVHM CDWPKVNEAY 800
LDPELEAAMS TARSIVEAAS NARQKAGRKL RWPVSRIVVS PESEDAAKAV 850
ERLRSVLMDQ TNSKAIVLTP VGESWDELGL EVIPDPSKIG PVFKKDAGKV 900
IPALQKVDGF ALKKAFAEAG EFELSLADRT TVTVTPGMAN FKETLPEGTA 950
SAESDAGLVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIRVS 1000
VRIEDERVLR LVETLKDLIA EEVRAEILNL GSDIDVSGAL VKDWDVEGIA 1050
MKMGISKK 1058
Length:1,058
Mass (Da):120,510
Last modified:September 13, 2005 - v1
Checksum:i81BE7252A232D33A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ72478.1.
RefSeqiYP_307058.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ72478; AAZ72478; Mbar_A3613.
GeneIDi3626315.
KEGGimba:Mbar_A3613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ72478.1 .
RefSeqi YP_307058.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q465G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A3613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ72478 ; AAZ72478 ; Mbar_A3613 .
GeneIDi 3626315.
KEGGi mba:Mbar_A3613.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MBAR269797:GHUW-3678-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiSYI_METBF
AccessioniPrimary (citable) accession number: Q465G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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