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Protein

NADP-reducing hydrogenase subunit HndC

Gene

hndD

Organism
Desulfovibrio fructosivorans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of NADP in the presence of molecular H2 to yield NADPH.2 Publications

Catalytic activityi

H2 + NADP+ = H+ + NADPH.

Enzyme regulationi

Inhibited by oxygen.1 Publication

Kineticsi

  1. KM=0.09 mM for NADP (at 30 degrees Celsius and at pH 8)1 Publication
  1. Vmax=0.013 µmol/min/mg enzyme (at 30 degrees Celsius and at pH 8)1 Publication

pH dependencei

Optimum pH is 8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi52Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi55Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi69Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-reducing hydrogenase subunit HndC (EC:1.12.1.3)
Alternative name(s):
Hydrogen dehydrogenase (NADP(+))
Gene namesi
Name:hndD
OrganismiDesulfovibrio fructosivorans
Taxonomic identifieri878 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004187181 – 585NADP-reducing hydrogenase subunit HndCAdd BLAST585

Interactioni

Subunit structurei

Heterotetramer composed of HndA, HndB, HndC and HndD subunits. HndD is probably the hydrogenase subunit.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ46508.
SMRiQ46508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 852Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST84
Domaini144 – 1744Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini185 – 2164Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107QHI. Bacteria.
COG3383. LUCA.
COG4624. LUCA.
KOiK18332.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
4.10.260.20. 1 hit.
InterProiView protein in InterPro
IPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu.
IPR013352. Fe_hydrogenase_subset.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
PfamiView protein in Pfam
PF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
PF00037. Fer4. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
SMARTiView protein in SMART
SM00902. Fe_hyd_SSU. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
SUPFAMiSSF53920. SSF53920. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR02512. FeFe_hydrog_A. 1 hit.
PROSITEiView protein in PROSITE
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q46508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMLTITIDG KTTSVPEGST ILDAAKTLDI DIPTLCYLNL EALSINNKAA
60 70 80 90 100
SCRVCVVEVE GRRNLAPSCA TPVTDNMVVK TNSLRVLNAR RTVLELLLSD
110 120 130 140 150
HPKDCLVCAK SGECELQTLA ERFGIRESPY DGGEMSHYRK DISASIIRDM
160 170 180 190 200
DKCIMCRRCE TMCNTVQTCG VLSGVNRGFT AVVAPAFEMN LADTVCTNCG
210 220 230 240 250
QCVAVCPTGA LVEHEYIWEV VEALANPDKV VIVQTAPAVR AALGEDLGVA
260 270 280 290 300
PGTSVTGKMA AALRRLGFDH VFDTDFAADL TIMEEGSEFL DRLGKHLAGD
310 320 330 340 350
TNVKLPILTS CCPGWVKFFE HQFPDMLDVP STAKSPQQMF GAIAKTYYAD
360 370 380 390 400
LLGIPREKLV VVSVMPCLAK KYECARPEFS VNGNPDVDIV ITTRELAKLV
410 420 430 440 450
KRMNIDFAGL PDEDFDAPLG ASTGAAPIFG VTGGVIEAAL RTAYELATGE
460 470 480 490 500
TLKKVDFEDV RGMDGVKKAK VKVGDNELVI GVAHGLGNAR ELLKPCGAGE
510 520 530 540 550
TFHAIEVMAC PGGCIGGGGQ PYHHGDVELL KKRTQVLYAE DAGKPLRKSH
560 570 580
ENPYIIELYE KFLGKPLSER SHQLLHTHYF KRQRL
Length:585
Mass (Da):63,429
Last modified:November 1, 1996 - v1
Checksum:iC14D0EF6AA3E2A72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07229 Genomic DNA. Translation: AAA87057.1.
PIRiD57150.

Genome annotation databases

KEGGiag:AAA87057.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHNDD_DESFR
AccessioniPrimary (citable) accession number: Q46508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program