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Protein

NADP-reducing hydrogenase subunit HndC

Gene

hndC

Organism
Desulfovibrio fructosivorans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of NADP in the presence of molecular H2 to yield NADPH.2 Publications

Catalytic activityi

H2 + NADP+ = H+ + NADPH.

Enzyme regulationi

Inhibited by oxygen.1 Publication

Kineticsi

  1. KM=0.09 mM for NADP (at 30 degrees Celsius and at pH 8)1 Publication
  1. Vmax=0.013 µmol/min/mg enzyme (at 30 degrees Celsius and at pH 8)1 Publication

pH dependencei

Optimum pH is 8.1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-reducing hydrogenase subunit HndC (EC:1.12.1.3)
Alternative name(s):
Hydrogen dehydrogenase (NADP(+))
Gene namesi
Name:hndC
OrganismiDesulfovibrio fructosivorans
Taxonomic identifieri878 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Pathology & Biotechi

Disruption phenotypei

Disruption completely abolishes the NADP reductase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004187171 – 490NADP-reducing hydrogenase subunit HndCAdd BLAST490

Interactioni

Subunit structurei

Heterotetramer composed of HndA, HndB, HndC and HndD subunits. HndC is probably the reducing subunit.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ46507
SMRiQ46507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini433 – 4624Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini463 – 4904Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST28

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107QIZ Bacteria
COG1145 LUCA
COG1894 LUCA
KOiK18331

Family and domain databases

Gene3Di1.20.1440.230, 1 hit
3.40.50.11540, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR001949 NADH-UbQ_OxRdtase_51kDa_CS
IPR011538 Nuo51_FMN-bd
IPR037225 Nuo51_FMN-bd_sf
IPR019575 Nuop51_4Fe4S-bd
IPR037207 Nuop51_4Fe4S-bd_sf
PfamiView protein in Pfam
PF01512 Complex1_51K, 1 hit
PF00037 Fer4, 2 hits
PF10589 NADH_4Fe-4S, 1 hit
SMARTiView protein in SMART
SM00928 NADH_4Fe-4S, 1 hit
SUPFAMiSSF140490 SSF140490, 1 hit
SSF142019 SSF142019, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 2 hits
PS00645 COMPLEX1_51K_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q46507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATTTEKKQ LRIATRNCGF IDPESIDDYI ALRGYEGLAK VLTMTPAEVV
60 70 80 90 100
DLVKRSGLRG RGGAGFPTGI KWGIALGNKA DQKYMVCNAD EGDPEFMDRA
110 120 130 140 150
VLEGDPHSVV EAMAIGGYAI GATRGTVYIR AEYPLAIKRL KKAIDDAREY
160 170 180 190 200
GLLGENIFGS GFDFDIELKY GAGAFVCGEE TALIRSMEGK RGEPVTKPPF
210 220 230 240 250
PAQSGYWEKP TIVNNVETFA NIPAIIINGA DWFSGIGTAT SKGTKVFALA
260 270 280 290 300
GKIQNVGLIE VPMGISLREV IFDIGGGCPD GKAFKAVQTG GPSGGALANK
310 320 330 340 350
DLDVAIDYES LAACKSIMGS GGMVVMDEDD CMVSVAKFFL DFTMDETCGK
360 370 380 390 400
CTPCRIGSKR LYEILDRITK GKGTRADLDR LKSLSEIIKD TALCGLGQTM
410 420 430 440 450
PNPILSTMDT FANEYEAHVD DKKCPAHVCT ALLTYTIDPA KCTGCGLCTR
460 470 480 490
VCPVECISGT KKQPHTIDTT RCIKCGACYD KCKFDSIIKQ
Length:490
Mass (Da):52,586
Last modified:November 1, 1996 - v1
Checksum:i60DFF36F0FA220A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07229 Genomic DNA Translation: AAA87056.1
PIRiC57150

Genome annotation databases

KEGGiag:AAA87056

Similar proteinsi

Entry informationi

Entry nameiHNDC_DESFR
AccessioniPrimary (citable) accession number: Q46507
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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