Q46482 (PUR2_ALLVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Alvin_0820

Organism

Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / D) (Chromatium vinosum) [Complete proteome] [HAMAP]

Taxonomic identifier

572477 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium › Chromatium vinosum

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151443
Regions
	Domain	109 – 316	208	ATP-grasp
	Nucleotide binding	135 – 196	62	ATP By similarity
Sites
	Metal binding	286	1	Magnesium or manganese By similarity
	Metal binding	288	1	Magnesium or manganese By similarity
Experimental info
	Sequence conflict	95 – 97	3	RQA → ASG in AAB02980. Ref.2
	Sequence conflict	159 – 160	2	DD → MI in AAB02980. Ref.2
	Sequence conflict	165 – 167	3	EAA → GGG in AAB02980. Ref.2
	Sequence conflict	174 – 183	10	GGRFGRAGAR → ADAFGPGGGG in AAB02980. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q46482 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 3CBFD73C9F913D37	FASTA	428	44,805
	10 20 30 40 50 60 MKILIVGSGG REHALAWKAA QSPQVEQVFV APGNGGTASE PGVENVAIAA DDIAGLVEFA 70 80 90 100 110 120 RRESIGLTIV GPEAPLVLGL VDAFAEAGLP CFGPRQASAQ LEGSKAFAKD FLHRHGIPTA 130 140 150 160 170 180 AYGVFTELEP ALAYLRQVGA PVVVKADGLA AGKGVILADD LATAEAAVHD MLGGGRFGRA 190 200 210 220 230 240 GARVVIEEFL TGEEASFIAM VDGRHILPLA SSQDHKARDD GDRGPNTGGM GAYSPAPIVT 250 260 270 280 290 300 PEIHDRIMRE VMEPTVAGLA AEGLPYLGFL YAGLMIGADG TPKVLEFNCR LGDPETQPLL 310 320 330 340 350 360 MRLQSDLVEL CLAALDGRLD QVTADWDARP ALGVVMAAGG YPDDYETGHV ISGLDAVPSS 370 380 390 400 410 420 EAKVFQAGTR CEGDAILTNG GRVLCVTALG ANVAEAQHLA YQAVDRIQWT DAFCRRDIGH RAIARERS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of chromosome of Allochromatium vinosum DSM 180." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Zigann R., Dahl C., Woyke T. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / NBRC 103801 / D.
[2]	Chen Y.L., Knaff D.B. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-189.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001896 Genomic DNA. Translation: ADC61767.1. L76417 Genomic DNA. Translation: AAB02980.1.
RefSeq	YP_003442799.1. NC_013851.1.
3D structure databases
ProteinModelPortal	Q46482.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADC61767; ADC61767; Alvin_0820.
GeneID	8786158.
KEGG	alv:Alvin_0820.
PATRIC	31921393. VBIAllVin64954_0819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000033463.
KO	K01945.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ALLVD

Accession

Primary (citable) accession number: Q46482
Secondary accession number(s): D3RQG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program