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Q46482 (PUR2_ALLVD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Alvin_0820
OrganismAllochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / D) (Chromatium vinosum) [Complete proteome] [HAMAP]
Taxonomic identifier572477 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeAllochromatium

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151443

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Experimental info

Sequence conflict95 – 973RQA → ASG in AAB02980. Ref.2
Sequence conflict159 – 1602DD → MI in AAB02980. Ref.2
Sequence conflict165 – 1673EAA → GGG in AAB02980. Ref.2
Sequence conflict174 – 18310GGRFGRAGAR → ADAFGPGGGG in AAB02980. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q46482 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 3CBFD73C9F913D37

FASTA42844,805
        10         20         30         40         50         60 
MKILIVGSGG REHALAWKAA QSPQVEQVFV APGNGGTASE PGVENVAIAA DDIAGLVEFA 

        70         80         90        100        110        120 
RRESIGLTIV GPEAPLVLGL VDAFAEAGLP CFGPRQASAQ LEGSKAFAKD FLHRHGIPTA 

       130        140        150        160        170        180 
AYGVFTELEP ALAYLRQVGA PVVVKADGLA AGKGVILADD LATAEAAVHD MLGGGRFGRA 

       190        200        210        220        230        240 
GARVVIEEFL TGEEASFIAM VDGRHILPLA SSQDHKARDD GDRGPNTGGM GAYSPAPIVT 

       250        260        270        280        290        300 
PEIHDRIMRE VMEPTVAGLA AEGLPYLGFL YAGLMIGADG TPKVLEFNCR LGDPETQPLL 

       310        320        330        340        350        360 
MRLQSDLVEL CLAALDGRLD QVTADWDARP ALGVVMAAGG YPDDYETGHV ISGLDAVPSS 

       370        380        390        400        410        420 
EAKVFQAGTR CEGDAILTNG GRVLCVTALG ANVAEAQHLA YQAVDRIQWT DAFCRRDIGH 


RAIARERS 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of chromosome of Allochromatium vinosum DSM 180."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Zigann R., Dahl C., Woyke T.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17899 / DSM 180 / NBRC 103801 / D.
[2]Chen Y.L., Knaff D.B.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-189.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001896 Genomic DNA. Translation: ADC61767.1.
L76417 Genomic DNA. Translation: AAB02980.1.
RefSeqYP_003442799.1. NC_013851.1.

3D structure databases

ProteinModelPortalQ46482.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC61767; ADC61767; Alvin_0820.
GeneID8786158.
KEGGalv:Alvin_0820.
PATRIC31921393. VBIAllVin64954_0819.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000033463.
KOK01945.

Enzyme and pathway databases

BioCycAVIN572477:GCJK-831-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_ALLVD
AccessionPrimary (citable) accession number: Q46482
Secondary accession number(s): D3RQG2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways