Q46480 (PUR9_ALLVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	Alvin_0822

Organism

Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / D) (Chromatium vinosum) [Complete proteome] [HAMAP]

Taxonomic identifier

572477 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Bifunctional purine biosynthesis protein PurH HAMAP MF_00139		PRO_0000192083

Sequences

	Sequence		Length	Mass (Da)
	Q46480 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: CB94BD901535C695	FASTA	519	55,955
	10 20 30 40 50 60 MQPIQRALIS VSDKTGLLDF ARVLAARGVE ILSTGGTARL LADNGLTVVE VSDHTGFPEM 70 80 90 100 110 120 MDGRVKTLHP RIHGGILGRR GLDDAVMTEH GIPPIDLVVV NLYPFEQTVA NPDCDLETAI 130 140 150 160 170 180 ENIDIGGPTL LRAAAKNHAS VTVVVDAADY ERVAEEIQSS GGVSEATRFD LAAKVFEHTA 190 200 210 220 230 240 RYDGAIANYL SARAESEAGA SFPRTLTLQF KRRQSMRYGE NPHQNAAFYV EHQVAEAGIS 250 260 270 280 290 300 TATQIQGKEL SYNNIADTDA ALECVKQFDE APACVIVKHA NPCGVAFGAT LLEAYDRAYQ 310 320 330 340 350 360 TDPESAFGGI IAFNRELDAE TAHAIVERQF VEVIIAPRVS DAARAAVASK PNVRLLECGD 370 380 390 400 410 420 WSREPGDRLD FKRVNGGLLV QDADLRLTDQ IRTVTERTPT EAELADLLFT WRVAKFVKSN 430 440 450 460 470 480 AIVYGRERMT IGVGAGQMSR VNSARIAAIK AEHAGLTVAG SVMASDAFFP FRDGIDQAAA 490 500 510 AGIKAVIQPG GSMRDAEVIA AANEHGMAMV FTGMRHFRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of chromosome of Allochromatium vinosum DSM 180." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Zigann R., Dahl C., Woyke T. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / NBRC 103801 / D.
[2]	Chen Y.L., Knaff D.B. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 442-519.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001896 Genomic DNA. Translation: ADC61769.1. L76417 Genomic DNA. Translation: AAB02978.1.
RefSeq	YP_003442801.1. NC_013851.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8786160.
GenomeReviews	Gene locus Alvin_0822 in contig CP001896_GR.
KEGG	alv:Alvin_0822.
PATRIC	31921397. VBIAllVin64954_0821.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.140.20. G3DSA:3.40.140.20. 2 hits. G3DSA:3.40.50.1380. MGS-like_dom. 1 hit.
KO	K00602.
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. PurH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_ALLVD

Accession

Primary (citable) accession number: Q46480
Secondary accession number(s): D3RQG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 15, 2010
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program