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Reviewed, UniProtKB/Swiss-Prot Q46444 (QHED_COMTE)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Quinohaemoprotein ethanol dehydrogenase type-1
    EC=1.1.99.-
Alternative name(s):
    Quinohaemoprotein ethanol dehydrogenase type I
    QH-EDH1
Gene names
Name: qheDH
OrganismComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifier285 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

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Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the dye-linked oxidation of primary alcohols to the corresponding aldehydes and the (subsequent) oxidation of the aldehydes to carboxylic acids.

Cofactor

Binds 1 PQQ group per subunit.

Binds 1 calcium ion per subunit.

Binds 1 heme group per subunit.

Subunit structure

Monomer.

Subcellular location

Periplasm Potential.

Induction

By ethanol and butanol.

Sequence similarities

Belongs to the bacterial PQQ dehydrogenase family.

Contains 1 cytochrome c domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Ref.2
Chain32 – 708677Quinohaemoprotein ethanol dehydrogenase type-1
PRO_0000025563

Regions

Domain619 – 70890Cytochrome c

Sites

Metal binding2161Calcium
Metal binding2941Calcium
Metal binding6391Iron (heme axial ligand)
Binding site6351Heme (covalent)
Binding site6381Heme (covalent)

Secondary structure

1 ..................................................................................................................................... 708
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q46444-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 99AB54BDD6ACCAB3

FASTA70876,823
        10         20         30         40         50         60 
MERLIDNSHG WPGRMVWLLA ACLGSAAAFA QTGPAAQAAA AVQRVDGDFI RANAARTPDW 

        70         80         90        100        110        120 
PTIGVDYAET RYSRLDQINA ANVKDLGLAW SYNLESTRGV EATPVVVDGI MYVSASWSVV 

       130        140        150        160        170        180 
HAIDTRTGNR IWTYDPQIDR STGFKGCCDV VNRGVALWKG KVYVGAWDGR LIALDAATGK 

       190        200        210        220        230        240 
EVWHQNTFEG QKGSLTITGA PRVFKGKVII GKRGAEYGVR GYITAYDAET GERKWRWFSV 

       250        260        270        280        290        300 
PGDPSKPFED ESMKRAARTW DPSGKWWEAG GGGTMWDSMT FDAELNTMYV GTGNGSPWSH 

       310        320        330        340        350        360 
KVRSPKGGDN LYLASIVALD PDTGKYKWHY QETPGDNWDY TSTQPMILAD IKIAGKPRKV 

       370        380        390        400        410        420 
ILHAPKNGFF FVLDRTNGKF ISAKNFVPVN WASGYDKHGK PIGIAAARDG SKPQDAVPGP 

       430        440        450        460        470        480 
YGAHNWHPMS FNPQTGLVYL PAQNVPVNLM DDKKWEFNQA GPGKPQSGTG WNTAKFFNAE 

       490        500        510        520        530        540 
PPKSKPFGRL LAWDPVAQKA AWSVEHVSPW NGGTLTTAGN VVFQGTADGR LVAYHAATGE 

       550        560        570        580        590        600 
KLWEAPTGTG VVAAPSTYMV DGRQYVSVAV GWGGVYGLAA RATERQGPGT VYTFVVAGKA 

       610        620        630        640        650        660 
RMPEFVAQRT GQLLQGVKYD PAKVEAGTML YVANCVFCHG VPGVDRGGNI PNLGYMDASY 

       670        680        690        700 
IENLPNFVFK GPAMVRGMPD FTGKLSGDDV ESLKAFIQGT ADAIRPKP

« Hide

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References

[1]"Characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of Comamonas testosteroni."
Stoorvogel J., Kraayveld D.E., van Sluis C.A., Jongejan J.A., De Vries S., Duine J.A.
Eur. J. Biochem. 235:690-698(1996) [PubMed: 8654419] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15667 / JCM 13048 / LMG 7106 / NCIB 9682.
[2]"Quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni. Purification, characterization, and reconstitution of the apoenzyme with pyrroloquinoline quinone analogues."
de Jong G.A.H., Geerlof A., Stoorvogel J., Jongejan J.A., De Vries S., Duine J.A.
Eur. J. Biochem. 230:899-905(1995) [PubMed: 7601151] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-54 AND 477-490.
Strain: ATCC 15667 / JCM 13048 / LMG 7106 / NCIB 9682.
[3]"Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni."
Groen B.W., van Kleef M.A., Duine J.A.
Biochem. J. 234:611-615(1986) [PubMed: 3521592] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Crystallization of quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroni: crystals with unique optical properties."
Oubrie A., Huizinga E.G., Rozeboom H.J., Kalk K.H., de Jong G.A.H., Duine J.A., Dijkstra B.W.
Acta Crystallogr. D 57:1732-1734(2001) [PubMed: 11679760] [Abstract]
Cited for: CRYSTALLIZATION.
[5]"Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate oxidation and electron transfer."
Oubrie A., Rozeboom H.J., Kalk K.H., Huizinga E.G., Dijkstra B.W.
J. Biol. Chem. 277:3727-3732(2002) [PubMed: 11714714] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS).

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Cross-references

Sequence databases

X81880 Genomic DNA. Translation: CAA57464.1.
PIRS52317. S62366.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1KB0X-ray1.44A32-708[»]
ModBaseSearch...

Family and domain databases

InterProIPR009056. Cyt_c_monohaem.
IPR019556. PQQ-dependent_C.
IPR019551. PQQ-dependent_N.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
Gene3DG3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
PfamPF01011. PQQ. 6 hits.
PF10535. PQQ_C. 2 hits.
PF10527. PQQ_N. 1 hit.
[Graphical view]
SMARTSM00564. PQQ. 5 hits.
[Graphical view]
TIGRFAMsTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEPS00364. BACTERIAL_PQQ_2. 1 hit.
PS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameQHED_COMTE
AccessionPrimary (citable) accession number: Q46444
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents