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Protein

Quinohemoprotein ethanol dehydrogenase type-1

Gene

qheDH

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dye-linked oxidation of primary alcohols to the corresponding aldehydes and the (subsequent) oxidation of the aldehydes to carboxylic acids.

Catalytic activityi

A primary alcohol + azurin = an aldehyde + reduced azurin.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi216Calcium1
Metal bindingi294Calcium1
Binding sitei635Heme (covalent)1
Binding sitei638Heme (covalent)1
Metal bindingi639Iron (heme axial ligand)1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, PQQ

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15520.
BRENDAi1.1.9.1. 1590.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinohemoprotein ethanol dehydrogenase type-1 (EC:1.1.9.1)
Alternative name(s):
Alcohol dehydrogenase (azurin)
QH-EDH1
Quinohemoprotein ethanol dehydrogenase type I
Gene namesi
Name:qheDH
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000002556332 – 708Quinohemoprotein ethanol dehydrogenase type-1Add BLAST677

Post-translational modificationi

In the crystallographic structures Trp-543 is oxidized to 2'-hydroxytryptophan.1 Publication

Expressioni

Inductioni

By ethanol and butanol.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi399795.CtesDRAFT_PD4908.

Structurei

Secondary structure

1708
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 43Combined sources11
Helixi47 – 52Combined sources6
Helixi53 – 55Combined sources3
Turni80 – 82Combined sources3
Helixi83 – 85Combined sources3
Beta strandi86 – 93Combined sources8
Beta strandi105 – 107Combined sources3
Beta strandi110 – 114Combined sources5
Helixi116 – 118Combined sources3
Beta strandi120 – 124Combined sources5
Turni125 – 127Combined sources3
Beta strandi130 – 134Combined sources5
Helixi140 – 145Combined sources6
Beta strandi146 – 148Combined sources3
Beta strandi156 – 158Combined sources3
Beta strandi161 – 165Combined sources5
Beta strandi169 – 175Combined sources7
Turni176 – 178Combined sources3
Beta strandi181 – 186Combined sources6
Turni187 – 190Combined sources4
Beta strandi202 – 204Combined sources3
Beta strandi207 – 210Combined sources4
Turni215 – 217Combined sources3
Beta strandi222 – 227Combined sources6
Turni228 – 230Combined sources3
Beta strandi233 – 240Combined sources8
Helixi251 – 257Combined sources7
Helixi262 – 264Combined sources3
Turni266 – 269Combined sources4
Beta strandi279 – 282Combined sources4
Turni283 – 286Combined sources4
Beta strandi287 – 291Combined sources5
Beta strandi295 – 298Combined sources4
Helixi300 – 303Combined sources4
Turni311 – 314Combined sources4
Beta strandi315 – 319Combined sources5
Turni321 – 323Combined sources3
Beta strandi326 – 333Combined sources8
Beta strandi347 – 353Combined sources7
Beta strandi356 – 363Combined sources8
Beta strandi368 – 374Combined sources7
Turni375 – 377Combined sources3
Beta strandi380 – 387Combined sources8
Beta strandi390 – 395Combined sources6
Beta strandi401 – 403Combined sources3
Helixi405 – 408Combined sources4
Beta strandi430 – 432Combined sources3
Turni433 – 436Combined sources4
Beta strandi437 – 444Combined sources8
Beta strandi448 – 451Combined sources4
Helixi467 – 469Combined sources3
Beta strandi475 – 477Combined sources3
Beta strandi487 – 494Combined sources8
Turni495 – 498Combined sources4
Beta strandi499 – 509Combined sources11
Beta strandi514 – 517Combined sources4
Turni518 – 520Combined sources3
Beta strandi521 – 525Combined sources5
Beta strandi529 – 535Combined sources7
Turni536 – 538Combined sources3
Beta strandi541 – 546Combined sources6
Beta strandi556 – 560Combined sources5
Beta strandi563 – 570Combined sources8
Helixi574 – 579Combined sources6
Beta strandi590 – 596Combined sources7
Helixi621 – 623Combined sources3
Helixi624 – 634Combined sources11
Helixi636 – 639Combined sources4
Turni642 – 644Combined sources3
Beta strandi648 – 650Combined sources3
Helixi653 – 655Combined sources3
Helixi658 – 662Combined sources5
Helixi664 – 668Combined sources5
Helixi674 – 676Combined sources3
Turni682 – 684Combined sources3
Helixi689 – 704Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KB0X-ray1.44A32-708[»]
ProteinModelPortaliQ46444.
SMRiQ46444.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46444.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini619 – 708Cytochrome cPROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the bacterial PQQ dehydrogenase family.Curated
Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
KOiK17760.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.140.10.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
PF13360. PQQ_2. 2 hits.
[Graphical view]
SMARTiSM00564. PQQ. 5 hits.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEiPS00364. BACTERIAL_PQQ_2. 1 hit.
PS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERLIDNSHG WPGRMVWLLA ACLGSAAAFA QTGPAAQAAA AVQRVDGDFI
60 70 80 90 100
RANAARTPDW PTIGVDYAET RYSRLDQINA ANVKDLGLAW SYNLESTRGV
110 120 130 140 150
EATPVVVDGI MYVSASWSVV HAIDTRTGNR IWTYDPQIDR STGFKGCCDV
160 170 180 190 200
VNRGVALWKG KVYVGAWDGR LIALDAATGK EVWHQNTFEG QKGSLTITGA
210 220 230 240 250
PRVFKGKVII GKRGAEYGVR GYITAYDAET GERKWRWFSV PGDPSKPFED
260 270 280 290 300
ESMKRAARTW DPSGKWWEAG GGGTMWDSMT FDAELNTMYV GTGNGSPWSH
310 320 330 340 350
KVRSPKGGDN LYLASIVALD PDTGKYKWHY QETPGDNWDY TSTQPMILAD
360 370 380 390 400
IKIAGKPRKV ILHAPKNGFF FVLDRTNGKF ISAKNFVPVN WASGYDKHGK
410 420 430 440 450
PIGIAAARDG SKPQDAVPGP YGAHNWHPMS FNPQTGLVYL PAQNVPVNLM
460 470 480 490 500
DDKKWEFNQA GPGKPQSGTG WNTAKFFNAE PPKSKPFGRL LAWDPVAQKA
510 520 530 540 550
AWSVEHVSPW NGGTLTTAGN VVFQGTADGR LVAYHAATGE KLWEAPTGTG
560 570 580 590 600
VVAAPSTYMV DGRQYVSVAV GWGGVYGLAA RATERQGPGT VYTFVVAGKA
610 620 630 640 650
RMPEFVAQRT GQLLQGVKYD PAKVEAGTML YVANCVFCHG VPGVDRGGNI
660 670 680 690 700
PNLGYMDASY IENLPNFVFK GPAMVRGMPD FTGKLSGDDV ESLKAFIQGT

ADAIRPKP
Length:708
Mass (Da):76,823
Last modified:November 1, 1996 - v1
Checksum:i99AB54BDD6ACCAB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81880 Genomic DNA. Translation: CAA57464.1.
PIRiS62366. S52317.

Genome annotation databases

KEGGiag:CAA57464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81880 Genomic DNA. Translation: CAA57464.1.
PIRiS62366. S52317.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KB0X-ray1.44A32-708[»]
ProteinModelPortaliQ46444.
SMRiQ46444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399795.CtesDRAFT_PD4908.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA57464.

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
KOiK17760.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15520.
BRENDAi1.1.9.1. 1590.

Miscellaneous databases

EvolutionaryTraceiQ46444.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.140.10.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
PF13360. PQQ_2. 2 hits.
[Graphical view]
SMARTiSM00564. PQQ. 5 hits.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEiPS00364. BACTERIAL_PQQ_2. 1 hit.
PS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQHED_COMTE
AccessioniPrimary (citable) accession number: Q46444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The oxidation form of Trp-543 is subject of controversy and could be the artifactual result of sample handling.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.