5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase

Preferred order of sections

Names and origin

Protein names

Submitted name:
5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase EMBL AAA53548.2

Gene names

Name:

acsE EMBL AAA53548.2

Organism

Moorella thermoacetica (Clostridium thermoaceticum) EMBL AAA53548.2

Taxonomic identifier

1525 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Moorella group › Moorella

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 2E7F PDB 2OGY Metal-binding PDB 2E7F PDB 2OGY
Molecular function	Methyltransferase EMBL AAA53548.2 Transferase
Technical term	3D-structure PDB 2E7F PDB 2OGY PDB 1F6Y
Gene Ontology (GO)
Biological process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

184

1

Calcium PDB 2E7F

Metal binding

222

1

Calcium; via carbonyl oxygen PDB 2E7F PDB 2OGY

Metal binding

224

1

Calcium PDB 2E7F PDB 2OGY

Sequences

Sequence

Length

Mass (Da)

Tools

Q46389 [UniParc].

Last modified October 1, 2001. Version 2.
Checksum: 62466A25D42496A1
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FASTA

262

28,609

        10         20         30         40         50         60 
MLIIGERING MFGDIKRAIQ ERDPAPVQEW ARRQEEGGAR ALDLNVGPAV QDKVSAMEWL 

        70         80         90        100        110        120 
VEVTQEVSNL TLCLDSTNIK AIEAGLKKCK NRAMINSTNA EREKVEKLFP LAVEHGAALI 

       130        140        150        160        170        180 
GLTMNKTGIP KDSDTRLAFA MELVAAADEF GLPMEDLYID PLILPANVAQ DHAPEVLKTL 

       190        200        210        220        230        240 
QQIKMLADPA PKTVLGLSNV SQNCQNRPLI NRTFLAMAMA CGLDAAIADA CDEALIETAA 

       250        260 
TAEILLNQTV YCDSFVKMFK TR

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References

[1]	"The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum." Roberts D.L., Zhao S., Doukov T., Ragsdale S.W. J. Bacteriol. 176:6127-6130(1994) [PubMed: 7928975] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 521 EMBL AAA53548.2.
[2]	Ragsdale S.W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 521 EMBL AAA53548.2.
[3]	"Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase." Doukov T., Seravalli J., Stezowski J.J., Ragsdale S.W. Structure 8:817-830(2000) [PubMed: 10997901] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
[4]	"Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer. Role of an active site asparagine residue in activation of methyl transfer by methyltransferases." Doukov T.I., Hemmi H., Drennan C.L., Ragsdale S.W. J. Biol. Chem. 282:6609-6618(2007) [PubMed: 17172470] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L34780 Genomic DNA. Translation: AAA53548.2.

PIR

I40795.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F6Y	X-ray	2.20	A/B	1-262	[»]
2E7F	X-ray	2.20	A/B	1-262	[»]
2OGY	X-ray	2.30	A/B	1-262	[»]

ProteinModelPortal

Q46389.

SMR

Q46389. Positions 1-262.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]

Gene3D

G3DSA:3.20.20.20. Dhdropt_synth. 1 hit.

Pfam

PF00809. Pterin_bind. 1 hit.
[Graphical view]

SUPFAM

SSF51717. DHP_synth_like. 1 hit.

PROSITE

PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q46389_MOOTH

Accession

Primary (citable) accession number: Q46389

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	October 1, 2001
Last modified:	July 27, 2011
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)