Reviewed, UniProtKB/Swiss-Prot Q46381 (BPHB_COMTE)

Names and origin

Protein names

Recommended name:
    Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
    EC=1.3.1.56
Alternative name(s):
    Biphenyl-2,3-dihydro-2,3-diol dehydrogenase
    Biphenyl-cis-diol dehydrogenase
    2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase
    2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase
    B2,3D

Gene names

Name:

bphB

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cis-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ = biphenyl-2,3-diol + NADH.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoic acid and benzoic acid from biphenyl: step 2/4.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase		PRO_0000054532
Regions
	Nucleotide binding	10 – 34	25	NAD By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46381-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D50A4CC9167A113E	FASTA	281	29,350
	10 20 30 40 50 60 MKLTGEVALI TGGASGLGRA LVDRFVAEGA RVAVLDKSAE RLRELEVAHG GNAVGVVGDV 70 80 90 100 110 120 RSLQDQKRAA ERCLAAFGKI DTLIPNAGIW DYSTALADLP EDKIDAAFDD IFHVNVKGYI 130 140 150 160 170 180 HAVKACLPAL VSSRGSVVFT ISNAGFYPNG GGPLYTATKH AVVGLVRQMA FELAPHVRVN 190 200 210 220 230 240 GVAPGGMNTD LRGPSSLGLS EQSISSVPLA DMLKSVLPIG RMPALEEYTG AYVFFATRGD 250 260 270 280 SLPATGALLN YDGGMGVRGF LTAAGGADLP EKLNINREGQ E

References

[1]

"Characterization of active recombinant 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB)." Sylvestre M., Hurtubise Y., Barriault D., Bergeron J., Ahmad D. Appl. Environ. Microbiol. 62:2710-2715(1996) [PubMed: 8702262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: B-356.

[2]

"Sequencing of Comamonas testosteroni strain B-356-biphenyl/chlorobiphenyl dioxygenase genes: evolutionary relationships among Gram-negative bacterial biphenyl dioxygenases." Sylvestre M., Sirois M., Hurtubise Y., Bergeron J., Ahmad D., Shareck F., Barriault D., Guillemette I., Juteau J.-M. Gene 174:195-202(1996) [PubMed: 8890734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15. Strain: B-356.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U57451 mRNA. Translation: AAB18304.1. U47637 Genomic DNA. Translation: AAC44530.1.
PIR	PC4213.
3D structure databases
HSSP	HSSP built from PDB template 1BDB based on UniProtKB P47227.
SMR	Q46381. Positions 1-276.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.56. 4566.
Family and domain databases
InterPro	IPR017711. BphB_TodD. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR03325. BphB_TodD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHB_COMTE

Accession

Primary (citable) accession number: Q46381
Secondary accession number(s): Q46376

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)