ID   BPHE_COMTE              Reviewed;         186 AA.
AC   Q46373;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-MAR-2009, entry version 46.
DE   RecName: Full=Biphenyl dioxygenase subunit beta;
DE            EC=1.14.12.18;
DE   AltName: Full=Biphenyl 2,3-dioxygenase;
GN   Name=bphE;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B-356;
RX   MEDLINE=97045812; PubMed=8890734; DOI=10.1016/0378-1119(96)00039-X;
RA   Sylvestre M., Sirois M., Hurtubise Y., Bergeron J., Ahmad D.,
RA   Shareck F., Barriault D., Guillemette I., Juteau J.-M.;
RT   "Sequencing of Comamonas testosteroni strain B-356-
RT   biphenyl/chlorobiphenyl dioxygenase genes: evolutionary relationships
RT   among Gram-negative bacterial biphenyl dioxygenases.";
RL   Gene 174:195-202(1996).
CC   -!- FUNCTION: The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY: Biphenyl + NADH + O(2) = (1S,2R)-3-
CC       phenylcyclohexa-3,5-diene-1,2-diol + NAD(+).
CC   -!- PATHWAY: Xenobiotic degradation; biphenyl degradation; 2-hydroxy-
CC       2,4-pentadienoic acid and benzoic acid from biphenyl: step 1/4.
CC   -!- SUBUNIT: Heterohexamer consisting of 3 BphA subunits and 3 BphE
CC       subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG)
CC       must be present to obtain activity.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; U47637; AAC44527.1; -; Genomic_DNA.
DR   PIR; JC4994; JC4994.
DR   BRENDA; 1.14.12.18; 4566.
DR   GO; GO:0018687; F:biphenyl 2,3-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase.
FT   CHAIN         1    186       Biphenyl dioxygenase subunit beta.
FT                                /FTId=PRO_0000085069.
SQ   SEQUENCE   186 AA;  21556 MW;  7A307552B4127A00 CRC64;
     MISTPLSKEF EWPAKPVSLE LQHQVEQFYY REAQLLDHHA FQAWFALLAE DIHYWMPIRT
     VRTAREQGLE YVPAGANAHF DDTHATMYGR IRQKTSDLNW AEDPPSRTRH LVSNVIVREM
     DTPGTLEVAS AFLLYRSRLE RQVDVFAGER RDVLRIADNP LGFQIAKRTI ILDQSTVLAN
     NLSVFF
//