Reviewed, UniProtKB/Swiss-Prot Q46373 (BPHE_COMTE)

Names and origin

Protein names

Recommended name:
    Biphenyl dioxygenase subunit beta
    EC=1.14.12.18
Alternative name(s):
    Biphenyl 2,3-dioxygenase

Gene names

Name:

bphE

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Biphenyl + NADH + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoic acid and benzoic acid from biphenyl: step 1/4.
Subunit structure	Heterohexamer consisting of 3 BphA subunits and 3 BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG) must be present to obtain activity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	biphenyl 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Biphenyl dioxygenase subunit beta		PRO_0000085069

Sequences

	Sequence		Length	Mass (Da)
	Q46373-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7A307552B4127A00	FASTA	186	21,556
	10 20 30 40 50 60 MISTPLSKEF EWPAKPVSLE LQHQVEQFYY REAQLLDHHA FQAWFALLAE DIHYWMPIRT 70 80 90 100 110 120 VRTAREQGLE YVPAGANAHF DDTHATMYGR IRQKTSDLNW AEDPPSRTRH LVSNVIVREM 130 140 150 160 170 180 DTPGTLEVAS AFLLYRSRLE RQVDVFAGER RDVLRIADNP LGFQIAKRTI ILDQSTVLAN NLSVFF

References

[1]

"Sequencing of Comamonas testosteroni strain B-356-biphenyl/chlorobiphenyl dioxygenase genes: evolutionary relationships among Gram-negative bacterial biphenyl dioxygenases." Sylvestre M., Sirois M., Hurtubise Y., Bergeron J., Ahmad D., Shareck F., Barriault D., Guillemette I., Juteau J.-M. Gene 174:195-202(1996) [PubMed: 8890734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B-356.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47637 Genomic DNA. Translation: AAC44527.1.
PIR	JC4994.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.12.18. 4566.
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHE_COMTE

Accession

Primary (citable) accession number: Q46373

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)