Q46373 (BPHE_COMTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biphenyl dioxygenase subunit beta
EC=1.14.12.18

Alternative name(s):
Biphenyl 2,3-dioxygenase

Gene names

Name:

bphE

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Biphenyl + NADH + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 1/4.
Subunit structure	Heterohexamer consisting of 3 BphA subunits and 3 BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG) must be present to obtain activity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	biphenyl 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 186

186

Biphenyl dioxygenase subunit beta

PRO_0000085069

Secondary structure

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186

Helix Strand Turn

Details...

Helix

19 – 37

19

Helix

41 – 45

5

Beta strand

48 – 57

10

Helix

64 – 66

3

Beta strand

78 – 82

5

Helix

84 – 87

4

Helix

91 – 94

4

Turn

95 – 97

3

Helix

100 – 102

3

Beta strand

107 – 119

13

Beta strand

126 – 138

13

Turn

139 – 141

3

Beta strand

142 – 146

5

Beta strand

150 – 155

6

Beta strand

164 – 170

7

Beta strand

175 – 177

3

Sequences

	Sequence		Length	Mass (Da)
	Q46373 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7A307552B4127A00	FASTA	186	21,556
	10 20 30 40 50 60 MISTPLSKEF EWPAKPVSLE LQHQVEQFYY REAQLLDHHA FQAWFALLAE DIHYWMPIRT 70 80 90 100 110 120 VRTAREQGLE YVPAGANAHF DDTHATMYGR IRQKTSDLNW AEDPPSRTRH LVSNVIVREM 130 140 150 160 170 180 DTPGTLEVAS AFLLYRSRLE RQVDVFAGER RDVLRIADNP LGFQIAKRTI ILDQSTVLAN NLSVFF

References

[1]

"Sequencing of Comamonas testosteroni strain B-356-biphenyl/chlorobiphenyl dioxygenase genes: evolutionary relationships among Gram-negative bacterial biphenyl dioxygenases." Sylvestre M., Sirois M., Hurtubise Y., Bergeron J., Ahmad D., Shareck F., Barriault D., Guillemette I., Juteau J.-M. Gene 174:195-202(1996) [PubMed: 8890734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B-356.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U47637 Genomic DNA. Translation: AAC44527.1.

PIR

JC4994.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GZX	X-ray	1.58	B	1-186	[»]
3GZY	X-ray	1.62	B	1-186	[»]

ProteinModelPortal

Q46373.

SMR

Q46373. Positions 6-186.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000391. Rng_hydr_dOase-bsu.
[Graphical view]

Pfam

PF00866. Ring_hydroxyl_B. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BPHE_COMTE

Accession

Primary (citable) accession number: Q46373

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 28, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program