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Protein
Submitted name:

Cytotoxin L

Gene
N/A
Organism
Clostridium sordellii
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391UDPCombined sources
Binding sitei265 – 2651UDPCombined sources
Binding sitei269 – 2691UDPCombined sources
Metal bindingi288 – 2881CalciumCombined sources
Binding sitei288 – 2881UDPCombined sources
Metal bindingi515 – 5151CalciumCombined sources
Metal bindingi518 – 5181CalciumCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1033UDPCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BRENDAi2.4.1.B62. 1514.

Protein family/group databases

CAZyiGT44. Glycosyltransferase Family 44.
MEROPSiC80.003.
TCDBi1.C.57.1.3. the clostridial cytotoxin (cct) family.

Names & Taxonomyi

Protein namesi
Submitted name:
Cytotoxin LImported
OrganismiClostridium sordelliiImported
Taxonomic identifieri1505 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePaeniclostridium

Interactioni

Protein-protein interaction databases

IntActiQ46342. 4 interactions.
MINTiMINT-7261718.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKDX-ray2.53A/B/C1-546[»]
2VKHX-ray2.30A/B/C1-546[»]
2VL8X-ray2.31A/B/C1-546[»]
ProteinModelPortaliQ46342.
SMRiQ46342. Positions 1-542.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46342.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini567 – 774208Peptidase C80InterPro annotationAdd
BLAST

Family and domain databases

InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. CPD_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]
PfamiPF01473. CW_binding_1. 5 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51771. CGT_MARTX_CPD. 1 hit.
PS51170. CW. 20 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLVNKAQLQ KMVYVKFRIQ EDEYVAILNA LEEYHNMSES SVVEKYLKLK
60 70 80 90 100
DINNLTDNYL NTYKKSGRNK ALKKFKEYLT MEVLELKNNS LTPVEKNLHF
110 120 130 140 150
IWIGGQINDT AINYINQWKD VNSDYTVKVF YDSNAFLINT LKKTIVESAT
160 170 180 190 200
NNTLESFREN LNDPEFDYNK FYRKRMEIIY DKQKHFIDYY KSQIEENPEF
210 220 230 240 250
IIDNIIKTYL SNEYSKDLEA LNKYIEESLN KITANNGNDI RNLEKFADED
260 270 280 290 300
LVRLYNQELV ERWNLAAASD ILRISMLKED GGVYLDVDIL PGIQPDLFKS
310 320 330 340 350
INKPDSITNT SWEMIKLEAI MKYKEYIPGY TSKNFDMLDE EVQRSFESAL
360 370 380 390 400
SSKSDKSEIF LPLDDIKVSP LEVKIAFANN SVINQALISL KDSYCSDLVI
410 420 430 440 450
NQIKNRYKIL NDNLNPSINE GTDFNTTMKI FSDKLASISN EDNMMFMIKI
460 470 480 490 500
TNYLKVGFAP DVRSTINLSG PGVYTGAYQD LLMFKDNSTN IHLLEPELRN
510 520 530 540 550
FEFPKTKISQ LTEQEITSLW SFNQARAKSQ FEEYKKGYFE GALGEDDNLD
560 570 580 590 600
FAQNTVLDKD YVSKKILSSM KTRNKEYIHY IVQLQGDKIS YEASCNLFSK
610 620 630 640 650
DPYSSILYQK NIEGSETAYY YYVADAEIKE IDKYRIPYQI SNKRNIKLTF
660 670 680 690 700
IGHGKSEFNT DTFANLDVDS LSSEIETILN LAKADISPKY IEINLLGCNM
710 720 730 740 750
FSYSISAEET YPGKLLLKIK DRVSELMPSI SQDSITVSAN QYEVRINEEG
760 770 780 790 800
KREILDHSGK WINKEESIIK DISSKEYISF NPKENKIIVK SKYLHELSTL
810 820 830 840 850
LQEIRNNANS SDIDLEKKVM LTECEINVAS NIDRQIVEGR IEEAKNLTSD
860 870 880 890 900
SINYIKNEFK LIESISDSLY DLKHQNGLDD SHFISFEDIS KTENGFRIRF
910 920 930 940 950
INKETGNSIF IETEKEIFSE YATHISKEIS NIKDTIFDNV NGKLVKKVNL
960 970 980 990 1000
DAAHEVNTLN SAFFIQSLIE YNTTKESLSN LSVAMKVQVY AQLFSTGLNT
1010 1020 1030 1040 1050
ITDASKVVEL VSTALDETID LLPTLSEGLP IIATIIDGVS LGAAIKELSE
1060 1070 1080 1090 1100
TNDPLLRQEI EAKIGIMAVN LTAASTAIVT SALGIASGFS ILLVPLAGIS
1110 1120 1130 1140 1150
AGIPSLVNNE LILQDKATKV IDYFKHISLA ETEGAFTLLD DKIIMPQDDL
1160 1170 1180 1190 1200
VLSEIDFNNN SITLGKCEIW RAEGGSGHTL TDDIDHFFSS PSITYRKPWL
1210 1220 1230 1240 1250
SIYDVLNIKK EKIDFSKDLM VLPNAPNRVF GYEMGWTPGF RSLDNDGTKL
1260 1270 1280 1290 1300
LDRIRDHYEG QFYWRYFAFI ADALITKLKP RYEDTNVRIN LDGNTRSFIV
1310 1320 1330 1340 1350
PVITTEQIRK NLSYSFYGSG GSYSLSLSPY NMNIDLNLVE NDTWVIDVDN
1360 1370 1380 1390 1400
VVKNITIESD EIQKGELIEN ILSKLNIEDN KIILNNHTIN FYGDINESNR
1410 1420 1430 1440 1450
FISLTFSILE DINIIIEIDL VSKSYKILLS GNCMKLIENS SDIQQKIDHI
1460 1470 1480 1490 1500
GFNGEHQKYI PYSYIDNETK YNGFIDYSKK EGLFTAEFSN ESIIRNIYMP
1510 1520 1530 1540 1550
DSNNLFIYSS KDLKDIRIIN KGDVKLLIGN YFKDDMKVSL SFTIEDTNTI
1560 1570 1580 1590 1600
KLNGVYLDEN GVAQILKFMN NAKSALNTSN SLMNFLESIN IKNIFYNNLD
1610 1620 1630 1640 1650
PNIEFILDTN FIISGSNSIG QFELICDKDK NIQPYFINFK IKETSYTLYV
1660 1670 1680 1690 1700
GNRQNLIVEP SYHLDDSGNI SSTVINFSQK YLYGIDRYVN KVIIAPNLYT
1710 1720 1730 1740 1750
DEINITPVYK PNYICPEVII LDANYINEKI NVNINDLSIR YVWDNDGSDL
1760 1770 1780 1790 1800
ILIANSEEDN QPQVKIRFVN VFKSDTAADK LSFNFSDKQD VSVSKIISTF
1810 1820 1830 1840 1850
SLAAYSDGFF DYEFGLVSLD NDYFYINSFG NMVSGLIYIN DSLYYFKPPK
1860 1870 1880 1890 1900
NNLITGFTTI DGNKYYFDPT KSGAASIGEI TIDGKDYYFN KQGILQVGVI
1910 1920 1930 1940 1950
NTSDGLKYFA PAGTLDENLE GESVNFIGKL NIDGKIYYFE DNYRAAVEWK
1960 1970 1980 1990 2000
LLDDETYYFN PKTGEALKGL HQIGDNKYYF DDNGIMQTGF ITINDKVFYF
2010 2020 2030 2040 2050
NNDGVMQVGY IEVNGKYFYF GKNGERQLGV FNTPDGFKFF GPKDDDLGTE
2060 2070 2080 2090 2100
EGELTLYNGI LNFNGKIYFF DISNTAVVGW GTLDDGSTYY FDDNRAEACI
2110 2120 2130 2140 2150
GLTVINDCKY YFDDNGIRQL GFITINDNIF YFSESGKIEL GYQNINGNYF
2160 2170 2180 2190 2200
YIDESGLVLI GVFDTPDGYK YFAPLNTVND NIYGQAVKYS GLVRVNEDVY
2210 2220 2230 2240 2250
YFGETYKIET GWIENETDKY YFDPETKKAY KGINVVDDIK YYFDENGIMR
2260 2270 2280 2290 2300
TGLISFENNN YYFNEDGKMQ FGYLNIKDKM FYFGKDGKMQ IGVFNTPDGF
2310 2320 2330 2340 2350
KYFAHQNTLD ENFEGESINY TGWLDLDGKR YYFTDEYIAA TGSLTIDGYN
2360
YYFDPDTAEL VVSE
Length:2,364
Mass (Da):270,580
Last modified:November 1, 1996 - v1
Checksum:iEAD8A4467A89BDBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82638 Genomic DNA. Translation: CAA57959.1.
PIRiI40884.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82638 Genomic DNA. Translation: CAA57959.1.
PIRiI40884.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKDX-ray2.53A/B/C1-546[»]
2VKHX-ray2.30A/B/C1-546[»]
2VL8X-ray2.31A/B/C1-546[»]
ProteinModelPortaliQ46342.
SMRiQ46342. Positions 1-542.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ46342. 4 interactions.
MINTiMINT-7261718.

Protein family/group databases

CAZyiGT44. Glycosyltransferase Family 44.
MEROPSiC80.003.
TCDBi1.C.57.1.3. the clostridial cytotoxin (cct) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.B62. 1514.

Miscellaneous databases

EvolutionaryTraceiQ46342.

Family and domain databases

InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. CPD_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]
PfamiPF01473. CW_binding_1. 5 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51771. CGT_MARTX_CPD. 1 hit.
PS51170. CW. 20 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B."
    Green G.A., Schue V., Monteil H.
    Gene 161:57-61(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 6018Imported.
  2. "Characterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii."
    Green G.A., Schue V., Girardot R., Monteil H.
    J. Med. Microbiol. 44:60-64(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 6018Imported.
  3. "Inhibition of the glucosyltransferase activity of clostridial Rho/Ras-glucosylating toxins by castanospermine."
    Jank T., Ziegler M.O., Schulz G.E., Aktories K.
    FEBS Lett. 582:2277-2282(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 1-546 IN COMPLEX WITH CALCIUM AND UDP.
  4. "Conformational changes and reaction of clostridial glycosylating toxins."
    Ziegler M.O., Jank T., Aktories K., Schulz G.E.
    J. Mol. Biol. 377:1346-1356(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-546 IN COMPLEX WITH CALCIUM.

Entry informationi

Entry nameiQ46342_CLOSO
AccessioniPrimary (citable) accession number: Q46342
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.