Q46342 (Q46342_CLOSO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Cytotoxin L EMBL CAA57959.1
Organism	Clostridium sordellii EMBL CAA57959.1
Taxonomic identifier	1505 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	2364 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2VL8 PDB 2VKD PDB 2VKH
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q46342 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EAD8A4467A89BDBB	FASTA	2,364	270,580
	10 20 30 40 50 60 MNLVNKAQLQ KMVYVKFRIQ EDEYVAILNA LEEYHNMSES SVVEKYLKLK DINNLTDNYL 70 80 90 100 110 120 NTYKKSGRNK ALKKFKEYLT MEVLELKNNS LTPVEKNLHF IWIGGQINDT AINYINQWKD 130 140 150 160 170 180 VNSDYTVKVF YDSNAFLINT LKKTIVESAT NNTLESFREN LNDPEFDYNK FYRKRMEIIY 190 200 210 220 230 240 DKQKHFIDYY KSQIEENPEF IIDNIIKTYL SNEYSKDLEA LNKYIEESLN KITANNGNDI 250 260 270 280 290 300 RNLEKFADED LVRLYNQELV ERWNLAAASD ILRISMLKED GGVYLDVDIL PGIQPDLFKS 310 320 330 340 350 360 INKPDSITNT SWEMIKLEAI MKYKEYIPGY TSKNFDMLDE EVQRSFESAL SSKSDKSEIF 370 380 390 400 410 420 LPLDDIKVSP LEVKIAFANN SVINQALISL KDSYCSDLVI NQIKNRYKIL NDNLNPSINE 430 440 450 460 470 480 GTDFNTTMKI FSDKLASISN EDNMMFMIKI TNYLKVGFAP DVRSTINLSG PGVYTGAYQD 490 500 510 520 530 540 LLMFKDNSTN IHLLEPELRN FEFPKTKISQ LTEQEITSLW SFNQARAKSQ FEEYKKGYFE 550 560 570 580 590 600 GALGEDDNLD FAQNTVLDKD YVSKKILSSM KTRNKEYIHY IVQLQGDKIS YEASCNLFSK 610 620 630 640 650 660 DPYSSILYQK NIEGSETAYY YYVADAEIKE IDKYRIPYQI SNKRNIKLTF IGHGKSEFNT 670 680 690 700 710 720 DTFANLDVDS LSSEIETILN LAKADISPKY IEINLLGCNM FSYSISAEET YPGKLLLKIK 730 740 750 760 770 780 DRVSELMPSI SQDSITVSAN QYEVRINEEG KREILDHSGK WINKEESIIK DISSKEYISF 790 800 810 820 830 840 NPKENKIIVK SKYLHELSTL LQEIRNNANS SDIDLEKKVM LTECEINVAS NIDRQIVEGR 850 860 870 880 890 900 IEEAKNLTSD SINYIKNEFK LIESISDSLY DLKHQNGLDD SHFISFEDIS KTENGFRIRF 910 920 930 940 950 960 INKETGNSIF IETEKEIFSE YATHISKEIS NIKDTIFDNV NGKLVKKVNL DAAHEVNTLN 970 980 990 1000 1010 1020 SAFFIQSLIE YNTTKESLSN LSVAMKVQVY AQLFSTGLNT ITDASKVVEL VSTALDETID 1030 1040 1050 1060 1070 1080 LLPTLSEGLP IIATIIDGVS LGAAIKELSE TNDPLLRQEI EAKIGIMAVN LTAASTAIVT 1090 1100 1110 1120 1130 1140 SALGIASGFS ILLVPLAGIS AGIPSLVNNE LILQDKATKV IDYFKHISLA ETEGAFTLLD 1150 1160 1170 1180 1190 1200 DKIIMPQDDL VLSEIDFNNN SITLGKCEIW RAEGGSGHTL TDDIDHFFSS PSITYRKPWL 1210 1220 1230 1240 1250 1260 SIYDVLNIKK EKIDFSKDLM VLPNAPNRVF GYEMGWTPGF RSLDNDGTKL LDRIRDHYEG 1270 1280 1290 1300 1310 1320 QFYWRYFAFI ADALITKLKP RYEDTNVRIN LDGNTRSFIV PVITTEQIRK NLSYSFYGSG 1330 1340 1350 1360 1370 1380 GSYSLSLSPY NMNIDLNLVE NDTWVIDVDN VVKNITIESD EIQKGELIEN ILSKLNIEDN 1390 1400 1410 1420 1430 1440 KIILNNHTIN FYGDINESNR FISLTFSILE DINIIIEIDL VSKSYKILLS GNCMKLIENS 1450 1460 1470 1480 1490 1500 SDIQQKIDHI GFNGEHQKYI PYSYIDNETK YNGFIDYSKK EGLFTAEFSN ESIIRNIYMP 1510 1520 1530 1540 1550 1560 DSNNLFIYSS KDLKDIRIIN KGDVKLLIGN YFKDDMKVSL SFTIEDTNTI KLNGVYLDEN 1570 1580 1590 1600 1610 1620 GVAQILKFMN NAKSALNTSN SLMNFLESIN IKNIFYNNLD PNIEFILDTN FIISGSNSIG 1630 1640 1650 1660 1670 1680 QFELICDKDK NIQPYFINFK IKETSYTLYV GNRQNLIVEP SYHLDDSGNI SSTVINFSQK 1690 1700 1710 1720 1730 1740 YLYGIDRYVN KVIIAPNLYT DEINITPVYK PNYICPEVII LDANYINEKI NVNINDLSIR 1750 1760 1770 1780 1790 1800 YVWDNDGSDL ILIANSEEDN QPQVKIRFVN VFKSDTAADK LSFNFSDKQD VSVSKIISTF 1810 1820 1830 1840 1850 1860 SLAAYSDGFF DYEFGLVSLD NDYFYINSFG NMVSGLIYIN DSLYYFKPPK NNLITGFTTI 1870 1880 1890 1900 1910 1920 DGNKYYFDPT KSGAASIGEI TIDGKDYYFN KQGILQVGVI NTSDGLKYFA PAGTLDENLE 1930 1940 1950 1960 1970 1980 GESVNFIGKL NIDGKIYYFE DNYRAAVEWK LLDDETYYFN PKTGEALKGL HQIGDNKYYF 1990 2000 2010 2020 2030 2040 DDNGIMQTGF ITINDKVFYF NNDGVMQVGY IEVNGKYFYF GKNGERQLGV FNTPDGFKFF 2050 2060 2070 2080 2090 2100 GPKDDDLGTE EGELTLYNGI LNFNGKIYFF DISNTAVVGW GTLDDGSTYY FDDNRAEACI 2110 2120 2130 2140 2150 2160 GLTVINDCKY YFDDNGIRQL GFITINDNIF YFSESGKIEL GYQNINGNYF YIDESGLVLI 2170 2180 2190 2200 2210 2220 GVFDTPDGYK YFAPLNTVND NIYGQAVKYS GLVRVNEDVY YFGETYKIET GWIENETDKY 2230 2240 2250 2260 2270 2280 YFDPETKKAY KGINVVDDIK YYFDENGIMR TGLISFENNN YYFNEDGKMQ FGYLNIKDKM 2290 2300 2310 2320 2330 2340 FYFGKDGKMQ IGVFNTPDGF KYFAHQNTLD ENFEGESINY TGWLDLDGKR YYFTDEYIAA 2350 2360 TGSLTIDGYN YYFDPDTAEL VVSE

References

[1]	"Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B." Green G.A., Schue V., Monteil H. Gene 161:57-61(1995) [PubMed: 7642137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 6018 EMBL CAA57959.1.
[2]	"Characterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii." Green G.A., Schue V., Girardot R., Monteil H. J. Med. Microbiol. 44:60-64(1996) [PubMed: 8544213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 6018 EMBL CAA57959.1.
[3]	"Inhibition of the glucosyltransferase activity of clostridial Rho/Ras-glucosylating toxins by castanospermine." Jank T., Ziegler M.O., Schulz G.E., Aktories K. FEBS Lett. 582:2277-2282(2008) [PubMed: 18505687] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 1-546.
[4]	"Conformational changes and reaction of clostridial glycosylating toxins." Ziegler M.O., Jank T., Aktories K., Schulz G.E. J. Mol. Biol. 377:1346-1356(2008) [PubMed: 18325534] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-546.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X82638 Genomic DNA. Translation: CAA57959.1.

PIR

I40884.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VKD	X-ray	2.53	A/B/C	1-546	[»]
2VKH	X-ray	2.30	A/B/C	1-546	[»]
2VL8	X-ray	2.31	A/B/C	1-546	[»]

ProteinModelPortal

Q46342.

SMR

Q46342. Positions 1-542.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-7261718.

Protein family/group databases

CAZy

GT44. Glycosyltransferase Family 44.

MEROPS

C80.003.

TCDB

1.C.57.1.3. clostridial cytotoxin (CCT) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. Pept_C80_RTX_self_cleaving.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]

Pfam

PF01473. CW_binding_1. 4 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]

PROSITE

PS51170. CW. 20 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q46342_CLOSO

Accession

Primary (citable) accession number: Q46342

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)