ID   SOXA_CORS1              Reviewed;         967 AA.
AC   Q46337;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Sarcosine oxidase subunit alpha;
DE            Short=Sarcosine oxidase subunit;
DE            EC=1.5.3.1;
GN   Name=soxA;
OS   Corynebacterium sp. (strain P-1).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=69006;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=95355441; PubMed=7543100; DOI=10.1074/jbc.270.31.18252;
RA   Chlumsky L.J., Zhang L., Jorns M.S.;
RT   "Sequence analysis of sarcosine oxidase and nearby genes reveals
RT   homologies with key enzymes of folate one-carbon metabolism.";
RL   J. Biol. Chem. 270:18252-18259(1995).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to
CC       yield glycine, hydrogen peroxide and 5,10-
CC       methylenetetrahydrofolate.
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: FAD.
CC   -!- SUBUNIT: Heterotetramer (alpha 100 kDa, beta 42 kDa, gamma 20 kDa,
CC       and delta 6 kDa).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the gcvT family.
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DR   EMBL; U23955; AAC43461.1; -; Genomic_DNA.
DR   SMR; Q46337; 8-967.
DR   BioCyc; MetaCyc:MON-8521; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:EC.
DR   GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   InterPro; IPR003953; FAD_bind2_N.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006277; SoxA.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   PRINTS; PR00368; FADPNR.
DR   TIGRFAMs; TIGR01372; soxA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN         1    967       Sarcosine oxidase subunit alpha.
FT                                /FTId=PRO_0000122627.
SQ   SEQUENCE   967 AA;  102767 MW;  C5F8A61F021134E9 CRC64;
     MSQNTSYRLP AEQSPAARID RGEALVLTVD GKQLEAFRGD TVASAMLANG QRACGNSMYL
     DRPRGIFSAG VEEPNALVTV EARHEQDINE SMLAATTVPV TANLSATLLR GLGVLDPSTD
     PAYYDHVHVH TDVLVVGAGP AGLAAAREAS RSGARVLLLD ERAEAGGSLR DAAGEQIDGQ
     DAAAWIDATV AELAAAEETT HLQRTTVLGS YDANYVVAVQ RRTVHLDGPS GAGVSRERIW
     HIRANQVVLA TGAHERPIVF ENNDRPGIML AGAVRSYLNC YGVRAGSQIV VATTNDSAYP
     LVADLAASGG VVAVIDARTT VSAAAAEAVG AGVRVITGSV VVDTEANESG ELSAVIVAEL
     GEDRELGEPQ RFEADVLAVA GGFNPVVHLH SQRQGKLVWD TSIHAFVPDA AVANQHLAGA
     LTGLFDTASA LSTGAAVGAA AATAAGFERI AQVPQALPVP AGETRPVWLV PSLNGDQATN
     YTTHFVDLQR DQTVSDVLRA TGAGLESVEH IKRYTSISTA NDQGKTSGVA AIGVIAAVLG
     IENPAEIGTT TFRAPYTPVA FAALAGRTRG ELLDPARITP MHSWHLAQGA KFEDVGQWKR
     AWYFPQDGED MDAAVYRECA AVRESVGMLD ATTLGKIEIR GADAAEFLNR IYTNGYTKLK
     VGMARYGVMC KADGMVFDDG VTLRLAEDRF LMHTTTGGAA GVLDWLEEWL QTEWPELDVT
     CTSVTEQLAT VAVVGPRSRD VVAKLVTGLD VSNDAFKFMS FQDVTLDSGI EARISRISFS
     GELAYEIAIP SWHGLRVWED VYAAGQEFNI TPYGTETMHV LRAEKGFIIV GQDTDGTVTP
     QDAGMEWVVS KLKDFVGKRS FSREDNLRED RKHLVSVLPV DTALRLAEGA ALVADGAVET
     EGCTPMEGWV TSSYNSPALG RTFGLALIKN GRSRIGEVLK TPVNGQLVDV LVSDLVLFDP
     EGSRRDG
//