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Q46289 (KPYK_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pykF
Ordered Locus Names:CPE2149
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Pyruvate kinase
PRO_0000112066

Sites

Metal binding341Potassium By similarity
Metal binding361Potassium By similarity
Metal binding661Potassium By similarity
Metal binding2211Magnesium By similarity
Metal binding2451Magnesium By similarity
Binding site321Substrate By similarity
Binding site2441Substrate; via amide nitrogen By similarity
Binding site2451Substrate; via amide nitrogen By similarity
Binding site2771Substrate By similarity
Site2191Transition state stabilizer By similarity

Experimental info

Sequence conflict171E → G in CAA60217. Ref.2
Sequence conflict451E → G in CAA60217. Ref.2
Sequence conflict571E → G in CAA60217. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q46289 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: 387970D750B2B39B

FASTA47452,082
        10         20         30         40         50         60 
MQKTKMIFTI GPSSDSEEIL REFIRIGMNA ARLNFSHGDH ASHKEKIELI KRLRKEEKSA 

        70         80         90        100        110        120 
TAILLDIKGP KIRTYNFKNG EAELKNGDEF TFSCGDEILG DNTKCSISYK ELYEDIKPGG 

       130        140        150        160        170        180 
SILVDDGLLE FKVKEVRGTD IICEVIEGGT IKDHKGVNVP NVPIKLPAVT EKDRSDLIFG 

       190        200        210        220        230        240 
CEMEVDFVAA SFIRKPEDVL EVREILDSHG GKDIKIISKI ESQEGVDNIK EIIKVTDGVM 

       250        260        270        280        290        300 
VARGDMGVEI PIENVPIIQK NIIKKCNQAG KIVITATQML DSMIRNPRPT RAEASDVCNA 

       310        320        330        340        350        360 
IFDGTDAIML SGESASGSFP IEAAMTMSRI AKKAEANLDY NYLLRRLKDP NPNPDAFADA 

       370        380        390        400        410        420 
ISYSASKTAS KFPTKAIVAA TQTGSTAKIL SKYKPSCPII AITPYEKVRR SLALNFGIIS 

       430        440        450        460        470 
KKCAYFNSTD EIIEEARKVA KEFEIAETGD NIMVAAGFPT SITGGTNMLK IEKI 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.
[2]"Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
Katayama S., Dupuy B., Garnier T., Cole S.T.
J. Bacteriol. 177:5680-5685(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
Strain: CPN50.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB81855.1.
X86495 Genomic DNA. Translation: CAA60217.1.
RefSeqNP_563065.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ46289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE2149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB81855; BAB81855; BAB81855.
GeneID990464.
KEGGcpe:CPE2149.
PATRIC19498265. VBICloPer59675_2222.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OMAHKEKIEL.
OrthoDBEOG6GBMB0.
ProtClustDBCLSK811177.

Enzyme and pathway databases

BioCycCPER195102:GJFM-2203-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_CLOPE
AccessionPrimary (citable) accession number: Q46289
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 31, 2002
Last modified: November 13, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways