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Q46289

- KPYK_CLOPE

UniProt

Q46289 - KPYK_CLOPE

Protein

Pyruvate kinase

Gene

pykF

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Metal bindingi34 – 341PotassiumBy similarity
    Metal bindingi36 – 361PotassiumBy similarity
    Metal bindingi66 – 661PotassiumBy similarity
    Sitei219 – 2191Transition state stabilizerBy similarity
    Metal bindingi221 – 2211MagnesiumBy similarity
    Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
    Metal bindingi245 – 2451MagnesiumBy similarity
    Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
    Binding sitei277 – 2771SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-2203-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pykF
    Ordered Locus Names:CPE2149
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Pyruvate kinasePRO_0000112066Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi195102.CPE2149.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiCMERAKP.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46289-1 [UniParc]FASTAAdd to Basket

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    MQKTKMIFTI GPSSDSEEIL REFIRIGMNA ARLNFSHGDH ASHKEKIELI    50
    KRLRKEEKSA TAILLDIKGP KIRTYNFKNG EAELKNGDEF TFSCGDEILG 100
    DNTKCSISYK ELYEDIKPGG SILVDDGLLE FKVKEVRGTD IICEVIEGGT 150
    IKDHKGVNVP NVPIKLPAVT EKDRSDLIFG CEMEVDFVAA SFIRKPEDVL 200
    EVREILDSHG GKDIKIISKI ESQEGVDNIK EIIKVTDGVM VARGDMGVEI 250
    PIENVPIIQK NIIKKCNQAG KIVITATQML DSMIRNPRPT RAEASDVCNA 300
    IFDGTDAIML SGESASGSFP IEAAMTMSRI AKKAEANLDY NYLLRRLKDP 350
    NPNPDAFADA ISYSASKTAS KFPTKAIVAA TQTGSTAKIL SKYKPSCPII 400
    AITPYEKVRR SLALNFGIIS KKCAYFNSTD EIIEEARKVA KEFEIAETGD 450
    NIMVAAGFPT SITGGTNMLK IEKI 474
    Length:474
    Mass (Da):52,082
    Last modified:January 31, 2002 - v2
    Checksum:i387970D750B2B39B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171E → G in CAA60217. (PubMed:7559358)Curated
    Sequence conflicti45 – 451E → G in CAA60217. (PubMed:7559358)Curated
    Sequence conflicti57 – 571E → G in CAA60217. (PubMed:7559358)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81855.1.
    X86495 Genomic DNA. Translation: CAA60217.1.
    RefSeqiNP_563065.1. NC_003366.1.
    WP_011010789.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB81855; BAB81855; BAB81855.
    GeneIDi990464.
    KEGGicpe:CPE2149.
    PATRICi19498265. VBICloPer59675_2222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81855.1 .
    X86495 Genomic DNA. Translation: CAA60217.1 .
    RefSeqi NP_563065.1. NC_003366.1.
    WP_011010789.1. NC_003366.1.

    3D structure databases

    ProteinModelPortali Q46289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 195102.CPE2149.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB81855 ; BAB81855 ; BAB81855 .
    GeneIDi 990464.
    KEGGi cpe:CPE2149.
    PATRICi 19498265. VBICloPer59675_2222.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi CMERAKP.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci CPER195102:GJFM-2203-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.
    2. "Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
      Katayama S., Dupuy B., Garnier T., Cole S.T.
      J. Bacteriol. 177:5680-5685(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
      Strain: CPN50.

    Entry informationi

    Entry nameiKPYK_CLOPE
    AccessioniPrimary (citable) accession number: Q46289
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3