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Reviewed, UniProtKB/Swiss-Prot Q46289 (KPYK_CLOPE)

Last modified June 16, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40
Gene names
Name: pykF
Ordered Locus Names: CPE2149
OrganismClostridium perfringens [Complete proteome] [HAMAP]
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

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Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Pyruvate kinase
PRO_0000112066

Sites

Active site2191 By similarity
Metal binding2211Magnesium By similarity
Metal binding2421Magnesium By similarity
Metal binding2431Magnesium By similarity

Experimental info

Sequence conflict171E → G in CAA60217. Ref.2
Sequence conflict451E → G in CAA60217. Ref.2
Sequence conflict571E → G in CAA60217. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q46289-1 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: 387970D750B2B39B

FASTA47452,082
        10         20         30         40         50         60 
MQKTKMIFTI GPSSDSEEIL REFIRIGMNA ARLNFSHGDH ASHKEKIELI KRLRKEEKSA 

        70         80         90        100        110        120 
TAILLDIKGP KIRTYNFKNG EAELKNGDEF TFSCGDEILG DNTKCSISYK ELYEDIKPGG 

       130        140        150        160        170        180 
SILVDDGLLE FKVKEVRGTD IICEVIEGGT IKDHKGVNVP NVPIKLPAVT EKDRSDLIFG 

       190        200        210        220        230        240 
CEMEVDFVAA SFIRKPEDVL EVREILDSHG GKDIKIISKI ESQEGVDNIK EIIKVTDGVM 

       250        260        270        280        290        300 
VARGDMGVEI PIENVPIIQK NIIKKCNQAG KIVITATQML DSMIRNPRPT RAEASDVCNA 

       310        320        330        340        350        360 
IFDGTDAIML SGESASGSFP IEAAMTMSRI AKKAEANLDY NYLLRRLKDP NPNPDAFADA 

       370        380        390        400        410        420 
ISYSASKTAS KFPTKAIVAA TQTGSTAKIL SKYKPSCPII AITPYEKVRR SLALNFGIIS 

       430        440        450        460        470 
KKCAYFNSTD EIIEEARKVA KEFEIAETGD NIMVAAGFPT SITGGTNMLK IEKI

« Hide

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References

« Hide 'large scale' references
[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.
[2]"Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
Katayama S., Dupuy B., Garnier T., Cole S.T.
J. Bacteriol. 177:5680-5685(1995) [PubMed: 7559358] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
Strain: CPN50.

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Cross-references

Sequence databases

BA000016 Genomic DNA. Translation: BAB81855.1.
X86495 Genomic DNA. Translation: CAA60217.1.
RefSeqNP_563065.1.

3D structure databases

HSSPHSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBaseSearch...

Genome annotation databases

GeneID990464.
GenomeReviewsGene locus CPE2149 in contig BA000016_GR.
KEGGcpe:CPE2149.
NMPDRfig|195102.1.peg.2212.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ46289.
OMAQ46289. PLRISKF.

Enzyme and pathway databases

BioCycCPER195102:CPE2149-MON.
BRENDA2.7.1.40. 2406.

Family and domain databases

InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase_cat.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
[Graphical view]
Gene3DG3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHERPTHR11817. Pyruvate_kinase. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
ProDomPD001009. Pyruvate_kinase. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameKPYK_CLOPE
AccessionPrimary (citable) accession number: Q46289
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 31, 2002
Last modified: June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents