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Protein

Pyruvate kinase

Gene

pykF

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA), Pyruvate kinase (pykA), Pyruvate kinase (pykF)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Metal bindingi34PotassiumBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi66PotassiumBy similarity1
Sitei219Transition state stabilizerBy similarity1
Metal bindingi221MagnesiumBy similarity1
Binding sitei244Substrate; via amide nitrogenBy similarity1
Metal bindingi245MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Binding sitei277SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pykF
Ordered Locus Names:CPE2149
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120661 – 474Pyruvate kinaseAdd BLAST474

Proteomic databases

PRIDEiQ46289

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ46289
SMRiQ46289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000021559
KOiK00873
OMAiICVEAEK
OrthoDBiPOG091H02AT

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q46289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKTKMIFTI GPSSDSEEIL REFIRIGMNA ARLNFSHGDH ASHKEKIELI
60 70 80 90 100
KRLRKEEKSA TAILLDIKGP KIRTYNFKNG EAELKNGDEF TFSCGDEILG
110 120 130 140 150
DNTKCSISYK ELYEDIKPGG SILVDDGLLE FKVKEVRGTD IICEVIEGGT
160 170 180 190 200
IKDHKGVNVP NVPIKLPAVT EKDRSDLIFG CEMEVDFVAA SFIRKPEDVL
210 220 230 240 250
EVREILDSHG GKDIKIISKI ESQEGVDNIK EIIKVTDGVM VARGDMGVEI
260 270 280 290 300
PIENVPIIQK NIIKKCNQAG KIVITATQML DSMIRNPRPT RAEASDVCNA
310 320 330 340 350
IFDGTDAIML SGESASGSFP IEAAMTMSRI AKKAEANLDY NYLLRRLKDP
360 370 380 390 400
NPNPDAFADA ISYSASKTAS KFPTKAIVAA TQTGSTAKIL SKYKPSCPII
410 420 430 440 450
AITPYEKVRR SLALNFGIIS KKCAYFNSTD EIIEEARKVA KEFEIAETGD
460 470
NIMVAAGFPT SITGGTNMLK IEKI
Length:474
Mass (Da):52,082
Last modified:January 31, 2002 - v2
Checksum:i387970D750B2B39B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17E → G in CAA60217 (PubMed:7559358).Curated1
Sequence conflicti45E → G in CAA60217 (PubMed:7559358).Curated1
Sequence conflicti57E → G in CAA60217 (PubMed:7559358).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA Translation: BAB81855.1
X86495 Genomic DNA Translation: CAA60217.1
RefSeqiWP_011010789.1, NC_003366.1

Genome annotation databases

EnsemblBacteriaiBAB81855; BAB81855; BAB81855
KEGGicpe:CPE2149

Similar proteinsi

Entry informationi

Entry nameiKPYK_CLOPE
AccessioniPrimary (citable) accession number: Q46289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 31, 2002
Last modified: May 23, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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