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Q46267

- PFLA_CLOPA

UniProt

Q46267 - PFLA_CLOPA

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Protein

Pyruvate formate-lyase-activating enzyme

Gene
act
Organism
Clostridium pasteurianum
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12104.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme
Gene namesi
Name:act
OrganismiClostridium pasteurianum
Taxonomic identifieri1501 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Pyruvate formate-lyase-activating enzymePRO_0000200529Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ46267.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46267-1 [UniParc]FASTAAdd to Basket

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MVMGRIHSIE SMGLVDGPGI RTVVFFQGCG LRCSYCHNPD TWNMAGGKEL    50
TAEELLKKLL RFKPYFDRSG GGVTFSGGEV LLQPEFLIDI LKLCKEQGIH 100
TAIDTAGYGY GNYEEILKHT DLVLLDIKHV DDDGYKCITG KGKRGFDDFL 150
KAVENIGVKV WIRHVIVPTL TDSKENIRKL ANIIKNIRNV EKVELLPYHT 200
LGINKYEKLN LDYKLRDIEA MDKEKRKKLE KYLKELLE 238
Length:238
Mass (Da):27,148
Last modified:November 1, 1996 - v1
Checksum:i85E2ACED85C6B7D1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X93463 Genomic DNA. Translation: CAA63749.1.
PIRiJC6011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X93463 Genomic DNA. Translation: CAA63749.1 .
PIRi JC6011.

3D structure databases

ProteinModelPortali Q46267.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12104.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum."
    Weidner G., Sawers G.
    J. Bacteriol. 178:2440-2444(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPFLA_CLOPA
AccessioniPrimary (citable) accession number: Q46267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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