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Protein
Submitted name:

Iota toxin component Ia

Gene
N/A
Organism
Clostridium perfringens
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei393 – 3931NADPCombined sources
Binding sitei421 – 4211NADPCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi336 – 3427NADCombined sources
Nucleotide bindingi336 – 3416NADPCombined sources
Nucleotide bindingi376 – 3794NADCombined sources
Nucleotide bindingi376 – 3794NADPCombined sources
Nucleotide bindingi390 – 3934NADCombined sources
Nucleotide bindingi419 – 4213NADCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

NADCombined sources, NADPCombined sources, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BRENDAi2.4.2.B12. 1503.
2.4.2.B13. 1503.

Names & Taxonomyi

Protein namesi
Submitted name:
Iota toxin component IaImported
OrganismiClostridium perfringensImported
Taxonomic identifieri1502 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 454426Sequence analysisPRO_5004232352Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-46105N.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIQX-ray1.80A/B42-454[»]
1GIRX-ray2.10A42-454[»]
3BUZX-ray2.81A42-454[»]
4GY2X-ray2.71A42-454[»]
4H03X-ray1.75A42-454[»]
4H0TX-ray2.20A42-454[»]
4H0VX-ray2.03A42-454[»]
4H0XX-ray2.33A42-454[»]
4H0YX-ray1.94A42-454[»]
ProteinModelPortaliQ46220.
SMRiQ46220. Positions 42-454.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46220.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 247189ADPrib_exo_ToxInterPro annotationAdd
BLAST
Domaini263 – 453191ADPrib_exo_ToxInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili51 – 8535Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coilSequence analysis, SignalSequence analysis

Family and domain databases

InterProiIPR003540. ADP-ribosyltransferase.
IPR016013. Binary_toxinA_clost-typ.
[Graphical view]
PfamiPF03496. ADPrib_exo_Tox. 2 hits.
[Graphical view]
PRINTSiPR01390. BINARYTOXINA.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVNKSISV FLILYLILTS SFPSYTYAQD LQIASNYITD RAFIERPEDF
60 70 80 90 100
LKDKENAIQW EKKEAERVEK NLDTLEKEAL ELYKKDSEQI SNYSQTRQYF
110 120 130 140 150
YDYQIESNPR EKEYKNLRNA ISKNKIDKPI NVYYFESPEK FAFNKEIRTE
160 170 180 190 200
NQNEISLEKF NELKETIQDK LFKQDGFKDV SLYEPGNGDE KPTPLLIHLK
210 220 230 240 250
LPKNTGMLPY INSNDVKTLI EQDYSIKIDK IVRIVIEGKQ YIKAEASIVN
260 270 280 290 300
SLDFKDDVSK GDLWGKENYS DWSNKLTPNE LADVNDYMRG GYTAINNYLI
310 320 330 340 350
SNGPLNNPNP ELDSKVNNIE NALKLTPIPS NLIVYRRSGP QEFGLTLTSP
360 370 380 390 400
EYDFNKIENI DAFKEKWEGK VITYPNFIST SIGSVNMSAF AKRKIILRIN
410 420 430 440 450
IPKDSPGAYL SAIPGYAGEY EVLLNHGSKF KINKVDSYKD GTVTKLILDA

TLIN
Length:454
Mass (Da):52,317
Last modified:November 1, 1996 - v1
Checksum:i5765009F6ADDEAD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73562 Genomic DNA. Translation: CAA51959.1.
PIRiI40861.
S68632.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73562 Genomic DNA. Translation: CAA51959.1.
PIRiI40861.
S68632.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIQX-ray1.80A/B42-454[»]
1GIRX-ray2.10A42-454[»]
3BUZX-ray2.81A42-454[»]
4GY2X-ray2.71A42-454[»]
4H03X-ray1.75A42-454[»]
4H0TX-ray2.20A42-454[»]
4H0VX-ray2.03A42-454[»]
4H0XX-ray2.33A42-454[»]
4H0YX-ray1.94A42-454[»]
ProteinModelPortaliQ46220.
SMRiQ46220. Positions 42-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46105N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.2.B12. 1503.
2.4.2.B13. 1503.

Miscellaneous databases

EvolutionaryTraceiQ46220.

Family and domain databases

InterProiIPR003540. ADP-ribosyltransferase.
IPR016013. Binary_toxinA_clost-typ.
[Graphical view]
PfamiPF03496. ADPrib_exo_Tox. 2 hits.
[Graphical view]
PRINTSiPR01390. BINARYTOXINA.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of Clostridium perfringens iota-toxin genes and expression in Escherichia coli."
    Perelle S., Gibert M., Boquet P., Popoff M.R.
    Infect. Immun. 61:5147-5156(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NCIB 10748Imported.
  2. Brabetz W., Brade H.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NCIB 10748Imported.
  3. "Crystal structure and site-directed mutagenesis of enzymatic components from Clostridium perfringens iota-toxin."
    Tsuge H., Nagahama M., Nishimura H., Hisatsune J., Sakaguchi Y., Itogawa Y., Katunuma N., Sakurai J.
    J. Mol. Biol. 325:471-483(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 42-454 IN COMPLEX WITH NAD AND NADP.
  4. "Structural basis of actin recognition and arginine ADP-ribosylation by Clostridium perfringens iota-toxin."
    Tsuge H., Nagahama M., Oda M., Iwamoto S., Utsunomiya H., Marquez V.E., Katunuma N., Nishizawa M., Sakurai J.
    Proc. Natl. Acad. Sci. U.S.A. 105:7399-7404(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 42-454.
  5. "Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex."
    Tsurumura T., Tsumori Y., Qiu H., Oda M., Sakurai J., Nagahama M., Tsuge H.
    Proc. Natl. Acad. Sci. U.S.A. 110:4267-4272(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 42-454 IN COMPLEX WITH NAD.

Entry informationi

Entry nameiQ46220_CLOPF
AccessioniPrimary (citable) accession number: Q46220
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.