ID   DAPF_CLOPE              Reviewed;         272 AA.
AC   Q46185;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2002, sequence version 2.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Diaminopimelate epimerase;
DE            Short=DAP epimerase;
DE            EC=5.1.1.7;
GN   Name=dapF; OrderedLocusNames=CPE1846;
OS   Clostridium perfringens.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1502;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   MEDLINE=21664373; PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A.,
RA   Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic
RT   flesh-eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-238.
RC   STRAIN=CPN50;
RX   MEDLINE=96032407; PubMed=7559358;
RA   Katayama S., Dupuy B., Garnier T., Cole S.T.;
RT   "Rapid expansion of the physical and genetic map of the chromosome of
RT   Clostridium perfringens CPN50.";
RL   J. Bacteriol. 177:5680-5685(1995).
CC   -!- CATALYTIC ACTIVITY: LL-2,6-diaminoheptanedioate = meso-
CC       diaminoheptanedioate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; DL-diaminopimelate from LL-diaminopimelate: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the diaminopimelate epimerase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA60229.1; Type=Frameshift; Positions=181;
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DR   EMBL; BA000016; BAB81552.1; -; Genomic_DNA.
DR   EMBL; X86511; CAA60229.1; ALT_FRAME; Genomic_DNA.
DR   RefSeq; NP_562762.1; -.
DR   HSSP; P44859; 1GQZ.
DR   GeneID; 990155; -.
DR   GenomeReviews; BA000016_GR; CPE1846.
DR   KEGG; cpe:CPE1846; -.
DR   NMPDR; fig|195102.1.peg.1909; -.
DR   HOGENOM; Q46185; -.
DR   OMA; Q46185; NIAFIEN.
DR   BioCyc; CPER195102:CPE1846-MON; -.
DR   BRENDA; 5.1.1.7; 2406.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008837; F:diaminopimelate epimerase activity; IEA:HAMAP.
DR   GO; GO:0009089; P:lysine biosynthetic process via diaminopime...; IEA:HAMAP.
DR   HAMAP; MF_00197; -; 1.
DR   InterPro; IPR001653; DAP_epimerase.
DR   InterPro; IPR018510; DAP_epimerase_CS.
DR   Pfam; PF01678; DAP_epimerase; 2.
DR   TIGRFAMs; TIGR00652; DapF; 1.
DR   PROSITE; PS01326; DAP_EPIMERASE; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Complete proteome; Cytoplasm; Isomerase;
KW   Lysine biosynthesis.
FT   CHAIN         1    272       Diaminopimelate epimerase.
FT                                /FTId=PRO_0000149835.
FT   ACT_SITE     72     72       By similarity.
FT   ACT_SITE    217    217       By similarity.
FT   CONFLICT    183    183       E -> D (in Ref. 2).
FT   CONFLICT    193    193       F -> S (in Ref. 2; CAA60229).
FT   CONFLICT    201    201       T -> P (in Ref. 2; CAA60229).
FT   CONFLICT    206    206       T -> P (in Ref. 2; CAA60229).
FT   CONFLICT    230    230       K -> N (in Ref. 2; CAA60229).
SQ   SEQUENCE   272 AA;  30084 MW;  60DB1A8527815C05 CRC64;
     MKFSKMHGNG NDFIVIEDLN NEYLGKEGEI AQKMCHRRFG IGADGILIVR KNENCDIEMV
     IINSDGSYAA MCGNGIRCFA KYVYEKGIVK KDVLDVLTGD GVKRIFLEIE NDKVKTINVN
     MGFGDFKPKN IPALCDEEII EKKVSVGNGN FEITSLLMGV PHTIIFEEEK YPIECGRDIE
     KYELFPQGTN VNFCKVIDRN TMEVRTWERG AGPTLACGTG NCASVIAANK LGLVDKEVKV
     IVPGGELKVN IEDDGVKMIG NASFICDGTY LF
//