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Q46185

- DAPF_CLOPE

UniProt

Q46185 - DAPF_CLOPE

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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111SubstrateUniRule annotation
Binding sitei45 – 451SubstrateUniRule annotation
Binding sitei63 – 631SubstrateUniRule annotation
Active sitei72 – 721Proton donor/acceptorUniRule annotation
Sitei162 – 1621Important for catalytic activityUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation
Sitei208 – 2081Important for catalytic activityUniRule annotation
Active sitei217 – 2171Proton donor/acceptorUniRule annotation

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCPER195102:GJFM-1894-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:CPE1846
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Diaminopimelate epimerasePRO_0000149835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 217

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi195102.CPE1846.

Structurei

3D structure databases

ProteinModelPortaliQ46185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 743Substrate bindingUniRule annotation
Regioni208 – 2092Substrate bindingUniRule annotation
Regioni218 – 2192Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiKMRIFNN.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46185-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFSKMHGNG NDFIVIEDLN NEYLGKEGEI AQKMCHRRFG IGADGILIVR
60 70 80 90 100
KNENCDIEMV IINSDGSYAA MCGNGIRCFA KYVYEKGIVK KDVLDVLTGD
110 120 130 140 150
GVKRIFLEIE NDKVKTINVN MGFGDFKPKN IPALCDEEII EKKVSVGNGN
160 170 180 190 200
FEITSLLMGV PHTIIFEEEK YPIECGRDIE KYELFPQGTN VNFCKVIDRN
210 220 230 240 250
TMEVRTWERG AGPTLACGTG NCASVIAANK LGLVDKEVKV IVPGGELKVN
260 270
IEDDGVKMIG NASFICDGTY LF
Length:272
Mass (Da):30,084
Last modified:January 31, 2002 - v2
Checksum:i60DB1A8527815C05
GO

Sequence cautioni

The sequence CAA60229.1 differs from that shown. Reason: Frameshift at position 181. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831E → D(PubMed:7559358)Curated
Sequence conflicti193 – 1931F → S in CAA60229. (PubMed:7559358)Curated
Sequence conflicti201 – 2011T → P in CAA60229. (PubMed:7559358)Curated
Sequence conflicti206 – 2061T → P in CAA60229. (PubMed:7559358)Curated
Sequence conflicti230 – 2301K → N in CAA60229. (PubMed:7559358)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81552.1.
X86511 Genomic DNA. Translation: CAA60229.1. Frameshift.
RefSeqiNP_562762.1. NC_003366.1.
WP_011010638.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB81552; BAB81552; BAB81552.
GeneIDi990155.
KEGGicpe:CPE1846.
PATRICi19497647. VBICloPer59675_1919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81552.1 .
X86511 Genomic DNA. Translation: CAA60229.1 . Frameshift.
RefSeqi NP_562762.1. NC_003366.1.
WP_011010638.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q46185.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE1846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB81552 ; BAB81552 ; BAB81552 .
GeneIDi 990155.
KEGGi cpe:CPE1846.
PATRICi 19497647. VBICloPer59675_1919.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi KMRIFNN.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CPER195102:GJFM-1894-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.
  2. "Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
    Katayama S., Dupuy B., Garnier T., Cole S.T.
    J. Bacteriol. 177:5680-5685(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-238.
    Strain: CPN50.

Entry informationi

Entry nameiDAPF_CLOPE
AccessioniPrimary (citable) accession number: Q46185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 31, 2002
Last modified: October 29, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3