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Reviewed, UniProtKB/Swiss-Prot Q46185 (DAPF_CLOPE)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Diaminopimelate epimerase
      Short name=DAP epimerase
    EC=5.1.1.7
Gene names
Name: dapF
Ordered Locus Names: CPE1846
OrganismClostridium perfringens [Complete proteome] [HAMAP]
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

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Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-diaminopimelate from LL-diaminopimelate: step 1/1. HAMAP MF_00197

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Sequence caution

The sequence CAA60229.1 differs from that shown. Reason: Frameshift at position 181.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Diaminopimelate epimerase HAMAP MF_00197
PRO_0000149835

Sites

Active site721 By similarity
Active site2171 By similarity

Experimental info

Sequence conflict1831E → D Ref.2
Sequence conflict1931F → S in CAA60229. Ref.2
Sequence conflict2011T → P in CAA60229. Ref.2
Sequence conflict2061T → P in CAA60229. Ref.2
Sequence conflict2301K → N in CAA60229. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q46185-1 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: 60DB1A8527815C05

FASTA27230,084
        10         20         30         40         50         60 
MKFSKMHGNG NDFIVIEDLN NEYLGKEGEI AQKMCHRRFG IGADGILIVR KNENCDIEMV 

        70         80         90        100        110        120 
IINSDGSYAA MCGNGIRCFA KYVYEKGIVK KDVLDVLTGD GVKRIFLEIE NDKVKTINVN 

       130        140        150        160        170        180 
MGFGDFKPKN IPALCDEEII EKKVSVGNGN FEITSLLMGV PHTIIFEEEK YPIECGRDIE 

       190        200        210        220        230        240 
KYELFPQGTN VNFCKVIDRN TMEVRTWERG AGPTLACGTG NCASVIAANK LGLVDKEVKV 

       250        260        270 
IVPGGELKVN IEDDGVKMIG NASFICDGTY LF

« Hide

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References

« Hide 'large scale' references
[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.
[2]"Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
Katayama S., Dupuy B., Garnier T., Cole S.T.
J. Bacteriol. 177:5680-5685(1995) [PubMed: 7559358] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-238.
Strain: CPN50.

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Cross-references

Sequence databases

BA000016 Genomic DNA. Translation: BAB81552.1.
X86511 Genomic DNA. Translation: CAA60229.1. Frameshift.
RefSeqNP_562762.1.

3D structure databases

HSSPHSSP built from PDB template 1GQZ based on UniProtKB P44859.
ModBaseSearch...

Genome annotation databases

GeneID990155.
GenomeReviewsGene locus CPE1846 in contig BA000016_GR.
KEGGcpe:CPE1846.
NMPDRfig|195102.1.peg.1909.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ46185.
OMAQ46185. NIAFIEN.

Enzyme and pathway databases

BioCycCPER195102:CPE1846-MON.
BRENDA5.1.1.7. 2406.

Family and domain databases

HAMAPMF_00197.
[Tree]
InterProIPR001653. DAP_epimerase.
IPR018510. DAP_epimerase_CS.
[Graphical view]
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDAPF_CLOPE
AccessionPrimary (citable) accession number: Q46185
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 31, 2002
Last modified: June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents