Q46185 (DAPF_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diaminopimelate epimerase
Short name=DAP epimerase
EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	CPE1846

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP MF_00197
Subcellular location	Cytoplasm By similarity HAMAP MF_00197.
Sequence similarities	Belongs to the diaminopimelate epimerase family.
Sequence caution	The sequence CAA60229.1 differs from that shown. Reason: Frameshift at position 181.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Diaminopimelate epimerase HAMAP MF_00197		PRO_0000149835
Sites
	Active site	72	1	By similarity
	Active site	217	1	By similarity
Experimental info
	Sequence conflict	183	1	E → D Ref.2
	Sequence conflict	193	1	F → S in CAA60229. Ref.2
	Sequence conflict	201	1	T → P in CAA60229. Ref.2
	Sequence conflict	206	1	T → P in CAA60229. Ref.2
	Sequence conflict	230	1	K → N in CAA60229. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q46185 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: 60DB1A8527815C05	FASTA	272	30,084
	10 20 30 40 50 60 MKFSKMHGNG NDFIVIEDLN NEYLGKEGEI AQKMCHRRFG IGADGILIVR KNENCDIEMV 70 80 90 100 110 120 IINSDGSYAA MCGNGIRCFA KYVYEKGIVK KDVLDVLTGD GVKRIFLEIE NDKVKTINVN 130 140 150 160 170 180 MGFGDFKPKN IPALCDEEII EKKVSVGNGN FEITSLLMGV PHTIIFEEEK YPIECGRDIE 190 200 210 220 230 240 KYELFPQGTN VNFCKVIDRN TMEVRTWERG AGPTLACGTG NCASVIAANK LGLVDKEVKV 250 260 270 IVPGGELKVN IEDDGVKMIG NASFICDGTY LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.
[2]	"Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50." Katayama S., Dupuy B., Garnier T., Cole S.T. J. Bacteriol. 177:5680-5685(1995) [PubMed: 7559358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-238. Strain: CPN50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81552.1. X86511 Genomic DNA. Translation: CAA60229.1. Frameshift.
RefSeq	NP_562762.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q46185.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	990155.
GenomeReviews	Gene locus CPE1846 in contig BA000016_GR.
KEGG	cpe:CPE1846.
NMPDR	fig|195102.1.peg.1909.
PATRIC	19497647. VBICloPer59675_1919.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG399442.
OMA	NCASVIA.
PhylomeDB	Q46185.
ProtClustDB	PRK00450.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1846-MONOMER.
Family and domain databases
HAMAP	MF_00197. DAP_epimerase. [Tree]
InterPro	IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. [Graphical view]
KO	K01778.
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_CLOPE

Accession

Primary (citable) accession number: Q46185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 31, 2002
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program