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Q46185

- DAPF_CLOPE

UniProt

Q46185 - DAPF_CLOPE

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111SubstrateUniRule annotation
    Binding sitei45 – 451SubstrateUniRule annotation
    Binding sitei63 – 631SubstrateUniRule annotation
    Active sitei72 – 721Proton donor/acceptorUniRule annotation
    Sitei162 – 1621Important for catalytic activityUniRule annotation
    Binding sitei190 – 1901SubstrateUniRule annotation
    Sitei208 – 2081Important for catalytic activityUniRule annotation
    Active sitei217 – 2171Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-1894-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:CPE1846
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Diaminopimelate epimerasePRO_0000149835Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi72 ↔ 217

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi195102.CPE1846.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 743Substrate bindingUniRule annotation
    Regioni208 – 2092Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKMRIFNN.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46185-1 [UniParc]FASTAAdd to Basket

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    MKFSKMHGNG NDFIVIEDLN NEYLGKEGEI AQKMCHRRFG IGADGILIVR    50
    KNENCDIEMV IINSDGSYAA MCGNGIRCFA KYVYEKGIVK KDVLDVLTGD 100
    GVKRIFLEIE NDKVKTINVN MGFGDFKPKN IPALCDEEII EKKVSVGNGN 150
    FEITSLLMGV PHTIIFEEEK YPIECGRDIE KYELFPQGTN VNFCKVIDRN 200
    TMEVRTWERG AGPTLACGTG NCASVIAANK LGLVDKEVKV IVPGGELKVN 250
    IEDDGVKMIG NASFICDGTY LF 272
    Length:272
    Mass (Da):30,084
    Last modified:January 31, 2002 - v2
    Checksum:i60DB1A8527815C05
    GO

    Sequence cautioni

    The sequence CAA60229.1 differs from that shown. Reason: Frameshift at position 181.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831E → D(PubMed:7559358)Curated
    Sequence conflicti193 – 1931F → S in CAA60229. (PubMed:7559358)Curated
    Sequence conflicti201 – 2011T → P in CAA60229. (PubMed:7559358)Curated
    Sequence conflicti206 – 2061T → P in CAA60229. (PubMed:7559358)Curated
    Sequence conflicti230 – 2301K → N in CAA60229. (PubMed:7559358)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81552.1.
    X86511 Genomic DNA. Translation: CAA60229.1. Frameshift.
    RefSeqiNP_562762.1. NC_003366.1.
    WP_011010638.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB81552; BAB81552; BAB81552.
    GeneIDi990155.
    KEGGicpe:CPE1846.
    PATRICi19497647. VBICloPer59675_1919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81552.1 .
    X86511 Genomic DNA. Translation: CAA60229.1 . Frameshift.
    RefSeqi NP_562762.1. NC_003366.1.
    WP_011010638.1. NC_003366.1.

    3D structure databases

    ProteinModelPortali Q46185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 195102.CPE1846.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB81552 ; BAB81552 ; BAB81552 .
    GeneIDi 990155.
    KEGGi cpe:CPE1846.
    PATRICi 19497647. VBICloPer59675_1919.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KMRIFNN.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci CPER195102:GJFM-1894-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.
    2. "Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50."
      Katayama S., Dupuy B., Garnier T., Cole S.T.
      J. Bacteriol. 177:5680-5685(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-238.
      Strain: CPN50.

    Entry informationi

    Entry nameiDAPF_CLOPE
    AccessioniPrimary (citable) accession number: Q46185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3