Q46149 (Q46149_CLONO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Alpha-toxin EMBL CAA88565.1
Organism	Clostridium novyi EMBL CAA88565.1
Taxonomic identifier	1542 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	2178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2VK9
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q46149 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9B0ADCE031C4A75A	FASTA	2,178	250,136
	10 20 30 40 50 60 MLITREQLMK IASIPLKRKE PEYNLILDAL ENFNRDIEGT SVKEIYSKLS KLNELVDNYQ 70 80 90 100 110 120 TKYPSSGRNL ALENFRDSLY SELRELIKNS RTSTIASKNL SFIWIGGPIS DQSLEYYNMW 130 140 150 160 170 180 KMFNKDYNIR LFYDKNSLLV NTLKTAIIQE SSKVIIEQNQ SNILDGTYGH NKFYSDRMKL 190 200 210 220 230 240 IYRYKRELKM LYENMKQNNS VDDIIINFLS NYFKYDIGKL NNQKENNNNK MIAIGATDIN 250 260 270 280 290 300 TENILTNKLK SYYYQELIQT NNLAAASDIL RIAILKKYGG VYCDLDFLPG VNLSLFNDIS 310 320 330 340 350 360 KPNGMDSNYW EAAIFEAIAN EKKLMNNYPY KYMEQVPSEI KERILSFVRN HDINDLILPL 370 380 390 400 410 420 GDIKISQLEI LLSRLKAATG KKTFSNAFII SNNDSLTLNN LISQLENRYE ILNSIIQEKF 430 440 450 460 470 480 KICETYDSYI NSVSELVLET TPKNLSMDGS SFYQQIIGYL SSGFKPEVNS TVFFSGPNIY 490 500 510 520 530 540 SSATCDTYHF IKNTFDMLSS QNQEIFEASN NLYFSKTHDE FKSSWLLRSN IAEKEFQKLI 550 560 570 580 590 600 KTYIGRTLNY EDGLNFNKWK RVTTSELLKV IEEVNSTKIY ENYDLNMILQ IQGDDISYES 610 620 630 640 650 660 AVNVFGKNPN KSILIQGVDD FANVFYFENG IVQSDNINNI LSRFNDIKKI KLTLIGHGEN 670 680 690 700 710 720 VFNPKLFGGK TVNDLYTNII KPKLQHLLER EGVILKNKYL KINILGCYMF TPKVDINSTF 730 740 750 760 770 780 VGKLFNKISR DLQPKGFSKN QLEISANKYA IRINREGKRE VLDYFGKWVS NTDLIAEQIS 790 800 810 820 830 840 NKYVVYWNEV ENTLSARVEQ LNKVAEFAKD INSIIQTTNN QELKQSLVNT YADLITTLYS 850 860 870 880 890 900 ELLKEDIPFE LDNIQIKERI ILNEISRLHD FSNIILDFYQ KNNISNNMII LFDSIIKEKD 910 920 930 940 950 960 YYNVKLANKI TGETSVIKTY SDSLWNFTNK YKKIVDDIKG IIVKDINGEF IKKADFEIEQ 970 980 990 1000 1010 1020 NPSLLNSAML MQLLIDYKPY TEILTNMNTS LKVQAYAQIF QLSIGAIQEA TEIVTIISDA 1030 1040 1050 1060 1070 1080 LNANFNILSK LKVGSSVASV IIDGINLIAA LTELKNVKTN FERKLIEAKV GMYSIGFILE 1090 1100 1110 1120 1130 1140 SSSLISGLLG ATAVSEILGV ISVPVAGILV GLPSLVNNIL VLGEKYNQIL DYFSKFYPIV 1150 1160 1170 1180 1190 1200 GKNPFSIQDN IIIPYDDIAI TELNFKYNKF KYGYAKISGL KVGLVTHIGE NIDHYFSAPS 1210 1220 1230 1240 1250 1260 LDHYIELSIY PALKLNDTNL PKGNVVLLPS GLNKVYKPEI SAIAGANSQE GNGVEVLNLI 1270 1280 1290 1300 1310 1320 RNYYVDSNGN TKFPWKYEAP FEYSFSYMRV EYFDTKVNVI LDNENKTLII PVLTIDEMRN 1330 1340 1350 1360 1370 1380 KISYEILGDG GQYNVILPVN QTNINIVSNK NDIWNFDVSY IVKESKIEDN KFVLDGFINN 1390 1400 1410 1420 1430 1440 IFSTLKVSND GFKIGKQFIS IKNTPRAINL SFKINNNIVI VSIYLNHEKS NSITIISSDL 1450 1460 1470 1480 1490 1500 NDIKNNFDNL LDNINYIGLG SISDNTINCI VRNDEVYMEG KIFLNEKKLV FIQNELELHL 1510 1520 1530 1540 1550 1560 YDSVNKDSQY LINNPINNVV KYKDGYIVEG TFLINSTENK YSLYIENNKI MLKGLYLESS 1570 1580 1590 1600 1610 1620 VFKTIQDKIY SKEKVNDYIL SLIKKFFTVN IQLCPFMIVS GVDENNRYLE YMLSTNNKWI 1630 1640 1650 1660 1670 1680 INGGYWENDF NNYKIVDFEK CNVIVSGSNK LNSEGDLADT IDVLDKDLEN LYIDSVIIIP 1690 1700 1710 1720 1730 1740 KVYTKKIIIH PIPNNPQINI INTQSIHDKC HLIIDSVLTN NYHWESDGDD LIITNGLDIN 1750 1760 1770 1780 1790 1800 IRILQGLSFG FKYKNIYLKF SNYDELSLND FLLQNYNVKG LYYINGELHY KNIPGDTFEY 1810 1820 1830 1840 1850 1860 GWINIDSRWY FFDSINLIAK KGYQEIEGER YYFNPNTGVQ ESGVFLTPNG LEYFTNKHAS 1870 1880 1890 1900 1910 1920 SKRWGRAINY TGWLTLDGNK YYFQSNSKAV TGLQKISDKY YYFNDNGQMQ IKWQIINNNK 1930 1940 1950 1960 1970 1980 YYFDGNTGEA IIGWFNNNKE RYYFDSEGRL LTGYQVIGDK SYYFSDNING NWEEGSGVLK 1990 2000 2010 2020 2030 2040 SGIFKTPSGF KLFSSEGDKS AINYKGWLDL NGNKYYFNSD SIAVTGSYNI KGIQYYFNPK 2050 2060 2070 2080 2090 2100 TAVLTNGWYT LDNNNYYVSN GHNVLGYQDI DGKGYYFDPS TGIQKAGVFP TPNGLRYFTM 2110 2120 2130 2140 2150 2160 KPIDGQRWGQ CIDYTGWLHL NGNKYYFGYY NSAVTGWRVL GGKRYFFNIK TGAATTGLLT 2170 LSGKRYYFNE KGEQLTLV

References

[1]	"Sequencing and analysis of the gene encoding the alpha-toxin of Clostridium novyi proves its homology to toxins A and B of Clostridium difficile." Hofmann F., Herrmann A., Habermann E., von Eichel-Streiber C. Mol. Gen. Genet. 247:670-679(1995) [PubMed: 7616958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC19402 EMBL CAA88565.1.
[2]	"Conformational changes and reaction of clostridial glycosylating toxins." Ziegler M.O., Jank T., Aktories K., Schulz G.E. J. Mol. Biol. 377:1346-1356(2008) [PubMed: 18325534] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 1-551.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z48636 Genomic DNA. Translation: CAA88565.1.

PIR

S55805.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VK9	X-ray	2.85	A	1-551	[»]

ProteinModelPortal

Q46149.

SMR

Q46149. Positions 2-541.

ModBase

Search...

Protein family/group databases

CAZy

GT44. Glycosyltransferase Family 44.

TCDB

1.C.57.1.4. clostridial cytotoxin (CCT) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. Pept_C80_RTX_self_cleaving.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]

Pfam

PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]

PROSITE

PS51170. CW. 14 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q46149_CLONO

Accession

Primary (citable) accession number: Q46149
Secondary accession number(s): Q46147, Q46148

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)