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Q46127 (SYW_CLOLO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Synonyms:trsA
OrganismClostridium longisporum
Taxonomic identifier1523 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140_B

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B
PRO_0000136622

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140_B
Motif201 – 2055"KMSKS" region HAMAP-Rule MF_00140_B

Sites

Binding site2041ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46127 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 692C820F5A08E4D1

FASTA34138,256
        10         20         30         40         50         60 
MAKEIILTGD RPTGKLHIGH YVGSLKNRVQ LQNSGDYRSF IMIADQQALT DNARNPEKIR 

        70         80         90        100        110        120 
NSLIEVALDY LAVGIDPLKS TILVQSQIPE LNELTMHYLN LVTLSRLERN PTVKAEIKQK 

       130        140        150        160        170        180 
NFENSIPAGF LIYPVSQAAD ITAFKATTVP VGEDQLPMIE QAREIVRSFN TIYGKEVLVE 

       190        200        210        220        230        240 
PKAVIPKGTI GRLPGTDGKA KMSKSIGNAI YLADEADVIK QKVMSMYTDP NHIKVTDPGQ 

       250        260        270        280        290        300 
VEGNTVFTYL DTFCKDTETL EEMKAHYSRG GLGDVKVKKF LNEILQAELE PIRNRRKEFQ 

       310        320        330        340 
KDIPEVYRIL KEGSEKAREV AAGTLKEVRE TIGIEYFNNI F 

« Hide

References

[1]Brown G.D., Thomson J.A.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B6405.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L49336 Genomic DNA. Translation: AAC05711.1.

3D structure databases

ProteinModelPortalQ46127.
SMRQ46127. Positions 2-336.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_CLOLO
AccessionPrimary (citable) accession number: Q46127
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries