Reviewed, UniProtKB/Swiss-Prot Q46127 (SYW_CLOLO)

Names and origin

Protein names

Recommended name:
    Tryptophanyl-tRNA synthetase
    EC=6.1.1.2
Alternative name(s):
    Tryptophan--tRNA ligase
      Short name=TrpRS

Gene names

Name:	trpS
Synonyms:	trsA

Organism

Clostridium longisporum

Taxonomic identifier

1523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00140
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Tryptophanyl-tRNA synthetase HAMAP MF_00140		PRO_0000136622
Regions
	Motif	12 – 20	9	"HIGH" region HAMAP MF_00140
	Motif	201 – 205	5	"KMSKS" region HAMAP MF_00140
Sites
	Binding site	204	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46127-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 692C820F5A08E4D1	FASTA	341	38,256
	10 20 30 40 50 60 MAKEIILTGD RPTGKLHIGH YVGSLKNRVQ LQNSGDYRSF IMIADQQALT DNARNPEKIR 70 80 90 100 110 120 NSLIEVALDY LAVGIDPLKS TILVQSQIPE LNELTMHYLN LVTLSRLERN PTVKAEIKQK 130 140 150 160 170 180 NFENSIPAGF LIYPVSQAAD ITAFKATTVP VGEDQLPMIE QAREIVRSFN TIYGKEVLVE 190 200 210 220 230 240 PKAVIPKGTI GRLPGTDGKA KMSKSIGNAI YLADEADVIK QKVMSMYTDP NHIKVTDPGQ 250 260 270 280 290 300 VEGNTVFTYL DTFCKDTETL EEMKAHYSRG GLGDVKVKKF LNEILQAELE PIRNRRKEFQ 310 320 330 340 KDIPEVYRIL KEGSEKAREV AAGTLKEVRE TIGIEYFNNI F

References

[1]	Brown G.D., Thomson J.A. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B6405.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L49336 Genomic DNA. Translation: AAC05711.1.
3D structure databases
HSSP	HSSP built from PDB template 1I6M based on UniProtKB P00953.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.2. 308341.
Family and domain databases
HAMAP	MF_00140. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-synth_Ib. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10055. Trp_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_CLOLO

Accession

Primary (citable) accession number: Q46127

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)