Q46124 (RPOB_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	Cj0478

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	1378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1378	1378	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047875
Experimental info
	Sequence conflict	338 – 347	10	NDLANGVDAA → MTWLMALMQP Ref.2
	Sequence conflict	558	1	A → R in CAA54509. Ref.2
	Sequence conflict	671	1	C → S in CAA54509. Ref.2
	Sequence conflict	691	1	A → R in CAA54509. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q46124 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: AB7467C305028EB5	FASTA	1,378	155,916
	10 20 30 40 50 60 MCDMLDNKLG NRLRVDFSNI SKQIEIPNLL QLQKKSFDYF LNLDNGESGI EKVFKSIFPI 70 80 90 100 110 120 HDPQNRLSLE YVSSEIGKPK YTIRECMERG LTYSVNLKMK IRLTLHEKDE KTGEKVGVKD 130 140 150 160 170 180 IKEQEIYIRE IPLMTDRVSF IINGVERVVV NQLHRSPGVI FKEEESSTVA NKLVYTAQII 190 200 210 220 230 240 PDRGSWLYFE YDAKDVLYVR INKRRKVPVT MLFRALGYKK QDIIKLFYPI QTIHVKKDKF 250 260 270 280 290 300 LTEFNPNDFM DRIEYDIKDE KGKIVHQAGK RLTKKKAEQL IKDGLKWIEY PVEILLNRYL 310 320 330 340 350 360 ANPIIDKESG EVLFDSLTLL DESKLAKIKE QKSFDIANDL ANGVDAAIIN SFAQDGETLK 370 380 390 400 410 420 LLKQSENIDD ENDLAAIRIY KVMRPGEPVV KDAAKAFVND LFFNPERYDL TKVGRMKMNH 430 440 450 460 470 480 KLGLEVPEYV TVLTNEDIIK TAKYLIKVKN GKGHIDDRDH LGNRRIRSIG ELLANELHLG 490 500 510 520 530 540 LAKMQKAIRD KFTSLNADLD KVMPYDLINP KMITTTIIEF FTGGQLSQFM DQTNPLSEVT 550 560 570 580 590 600 HKRRLSALGE GGLVKERAGF EVRDVHATHY GRICPVETPE GQNIGLINTL STYAKVNELG 610 620 630 640 650 660 FVEAPYRKVV NGKVTNEVVY LTATQEEGLF IAPASTKVDA KGNIVEEFVE ARQDGETILA 670 680 690 700 710 720 RREEVQLIDL CSGMVVGVAA SLIPFLEHDD ANRALMGSNM QRQAVPLLTA SAPIVGTGME 730 740 750 760 770 780 QIIARDAWEA VKAKRGGVVE KVDNKSIFIL GEDDKGPFID HYTMEKNLRT NQNTNYIQHP 790 800 810 820 830 840 IVKKGDIVKA GQIIADGPSM DQGELAIGKN ALIAFMPWNG YNYEDAIVVS ERIIREDTFT 850 860 870 880 890 900 SVHIYEKEIE ARELKDGIEE ITKDIPNVKE EDVAHLDESG IAKIGTHIKP GMILVGKVSP 910 920 930 940 950 960 KGEVKPTPEE RLLRAIFGEK AGHVVNKSLY ATASLEGVVV DVKIFTKKGY EKDDRAIKSY 970 980 990 1000 1010 1020 DKEKMALEKE HHDRLLMMDR EEMLRVCALL SKASLNSDQK IGDKNYKKGQ TADISELEKI 1030 1040 1050 1060 1070 1080 NRFTLTTLIK AYSKEIQKEY DDLKNHFQNE KKKLKAEHDE KLEILEKDDI LPSGVIKLVK 1090 1100 1110 1120 1130 1140 VYIATKRKLK VGDKMAGRHG NKGIVSTIVP EVDMPYLPNG KSVDIALNPL GVPSRMNIGQ 1150 1160 1170 1180 1190 1200 ILESHLGLVG LRLGDQIQEI FDRKQKDFLK ELRAKILEIC SIPRLANEKE FIKSLSDEEL 1210 1220 1230 1240 1250 1260 LNYARDWSKG VKFSTPVFEG VNIEEFSKLF KMAKIDMDGK TELYDGRTGE KIAERVHVGC 1270 1280 1290 1300 1310 1320 MYMLKLHHLV DEKVHARSTG PYSLVTQQPV GGKALFGGQR FGEMEVWALE AYGAAHTLRE 1330 1340 1350 1360 1370 MLTIKSDDVE GRFSAYKALT KGENVPATGI PETFFVLTNE LKSLALDVEI FDKDEDNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.
[2]	"Identification of Campylobacter jejuni and C.coli using the rpoB gene and a cryptic DNA fragment from C.jejuni." Bustamante V.H., Puente J.L., Sanchez-Lopez F., Bobadilla M., Calva E. Gene 165:1-8(1995) [PubMed: 7489896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-1031.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34626.1. X77304 Genomic DNA. Translation: CAA54509.1.
PIR	A81393. S41868.
RefSeq	YP_002343912.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q46124.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904806.
GenomeReviews	Gene locus Cj0478 in contig AL111168_GR.
KEGG	cje:Cj0478.
PATRIC	20057893. VBICamJej33762_0470.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	VDARDTK.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0478-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_CAMJE

Accession

Primary (citable) accession number: Q46124
Secondary accession number(s): Q0PB35, Q9PI31

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program