Reviewed, UniProtKB/Swiss-Prot Q460N3 (PAR15_HUMAN)

Names and origin

Protein names

Recommended name:
    Poly [ADP-ribose] polymerase 15
      Short name=PARP-15
    EC=2.4.2.30
Alternative name(s):
    B-aggressive lymphoma protein 3

Gene names

Name:	PARP15
Synonyms:	BAL3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional repressor. Has ADP-ribosyltransferase activity. Ref.1
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 2 Macro domains. Contains 1 PARP catalytic domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	NAD
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q460N3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q460N3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-212: Missing.
     213-234: RPITSPLQEVHFLVYTNDDEGC → MLQRIGLIFLHNIVVVSNCFYF

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 656

656

Poly [ADP-ribose] polymerase 15

PRO_0000252436

Regions

Domain

56 – 245

190

Macro 1

Domain

271 – 442

172

Macro 2

Domain

460 – 656

197

PARP catalytic

Natural variations

Alternative sequence

1 – 212

212

Missing in isoform 2.

VSP_020971

Alternative sequence

213 – 234

22

RPITS…DDEGC → MLQRIGLIFLHNIVVVSNCF YF in isoform 2.

VSP_020972

Natural variant

315

1

R → K: dbSNP rs6793271.

VAR_027862

Natural variant

499

1

A → T: dbSNP rs34383355.

VAR_056658

Natural variant

606

1

G → R: dbSNP rs12489170.

VAR_027863

Secondary structure

1

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656

Helix Strand Turn

Details...

Beta strand

471 – 476

6

Helix

482 – 495

14

Beta strand

498 – 508

11

Helix

509 – 525

17

Beta strand

526 – 528

3

Beta strand

532 – 539

8

Helix

541 – 543

3

Helix

544 – 550

7

Turn

554 – 556

3

Beta strand

566 – 573

8

Helix

574 – 578

5

Turn

580 – 582

3

Beta strand

591 – 599

9

Beta strand

602 – 605

4

Beta strand

615 – 617

3

Beta strand

626 – 630

5

Beta strand

632 – 634

3

Beta strand

636 – 640

5

Beta strand

645 – 655

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 98391471221A3EAF	FASTA	656	72,541
	10 20 30 40 50 60 MHGVAGVTSR AGRDREAGSM LPAGNRGARK ASRRSSSRSM SRDNKFSKKD CLSIRNVVAS 70 80 90 100 110 120 IQTKEGLNLK LISGDVLYIW ADVIVNSVPM NLQLGGGPLS RAFLQKAGPM LQKELDDRRR 130 140 150 160 170 180 ETEEKVGNIF MTSGCNLDCK AVLHAVAPYW NNGAETSWQI MANIIKKCLT TVEVLSFSSI 190 200 210 220 230 240 TFPMIGTGSL QFPKAVFAKL ILSEVFEYSS STRPITSPLQ EVHFLVYTND DEGCQAFLDE 250 260 270 280 290 300 FTNWSRINPN KARIPMAGDT QGVVGTVSKP CFTAYEMKIG AITFQVATGD IATEQVDVIV 310 320 330 340 350 360 NSTARTFNRK SGVSRAILEG AGQAVESECA VLAAQPHRDF IITPGGCLKC KIIIHVPGGK 370 380 390 400 410 420 DVRKTVTSVL EECEQRKYTS VSLPAIGTGN AGKNPITVAD NIIDAIVDFS SQHSTPSLKT 430 440 450 460 470 480 VKVVIFQPEL LNIFYDSMKK RDLSASLNFQ STFSMTTCNL PEHWTDMNHQ LFCMVQLEPG 490 500 510 520 530 540 QSEYNTIKDK FTRTCSSYAI EKIERIQNAF LWQSYQVKKR QMDIKNDHKN NERLLFHGTD 550 560 570 580 590 600 ADSVPYVNQH GFNRSCAGKN AVSYGKGTYF AVDASYSAKD TYSKPDSNGR KHMYVVRVLT 610 620 630 640 650 GVFTKGRAGL VTPPPKNPHN PTDLFDSVTN NTRSPKLFVV FFDNQAYPEY LITFTA
	Isoform 2. Checksum: 1A86C67A336419FC	FASTA	444	49,501
	10 20 30 40 50 60 MLQRIGLIFL HNIVVVSNCF YFQAFLDEFT NWSRINPNKA RIPMAGDTQG VVGTVSKPCF 70 80 90 100 110 120 TAYEMKIGAI TFQVATGDIA TEQVDVIVNS TARTFNRKSG VSRAILEGAG QAVESECAVL 130 140 150 160 170 180 AAQPHRDFII TPGGCLKCKI IIHVPGGKDV RKTVTSVLEE CEQRKYTSVS LPAIGTGNAG 190 200 210 220 230 240 KNPITVADNI IDAIVDFSSQ HSTPSLKTVK VVIFQPELLN IFYDSMKKRD LSASLNFQST 250 260 270 280 290 300 FSMTTCNLPE HWTDMNHQLF CMVQLEPGQS EYNTIKDKFT RTCSSYAIEK IERIQNAFLW 310 320 330 340 350 360 QSYQVKKRQM DIKNDHKNNE RLLFHGTDAD SVPYVNQHGF NRSCAGKNAV SYGKGTYFAV 370 380 390 400 410 420 DASYSAKDTY SKPDSNGRKH MYVVRVLTGV FTKGRAGLVT PPPKNPHNPT DLFDSVTNNT 430 440 RSPKLFVVFF DNQAYPEYLI TFTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"B-aggressive lymphoma family proteins have unique domains that modulate transcription and exhibit poly(ADP-ribose) polymerase activity." Aguiar R.C.T., Takeyama K., He C., Kreinbrink K., Shipp M.A. J. Biol. Chem. 280:33756-33765(2005) [PubMed: 16061477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ063586 mRNA. Translation: AAY64451.1.
AK097916 mRNA. Translation: BAC05197.1.
BC101703 mRNA. Translation: AAI01704.1.
BC101701 mRNA. Translation: AAI01702.1.

IPI

IPI00746210.
IPI00945048.

RefSeq

NP_001106995.1.
NP_689828.1.

UniGene

Hs.120250

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BLJ	X-ray	2.20	A/B	459-656	[»]
3GEY	X-ray	2.20	A/B/C/D	459-656	[»]
3KH6	X-ray	2.20	A	273-448	[»]

SMR

Q460N3. Positions 65-245, 282-442.

ModBase

Search...

Protein-protein interaction databases

STRING

Q460N3.

Proteomic databases

PRIDE

Q460N3.

Genome annotation databases

Ensembl

ENST00000383660; ENSP00000373156; ENSG00000173200; Homo sapiens. [Genome view]
ENST00000464300; ENSP00000417214; ENSG00000173200; Homo sapiens. [Genome view]

GeneID

165631.

KEGG

hsa:165631.

UCSC

uc003efm.2. human.
uc003efo.1. human.

Organism-specific databases

CTD

165631.

GeneCards

GC03P123817.

HGNC

HGNC:26876. PARP15.

MIM

612066. gene.

PharmGKB

PA134905094.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09155.

HOGENOM

HBG506517.

InParanoid

Q460N3.

Enzyme and pathway databases

BRENDA

2.4.2.30. 247.

Gene expression databases

ArrayExpress

Q460N3.

Bgee

Q460N3.

CleanEx

HS_PARP15.

Genevestigator

Q460N3.

GermOnline

ENSG00000173200. Homo sapiens.

Family and domain databases

InterPro

IPR002589. A1pp.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
[Graphical view]

Pfam

PF01661. Macro. 2 hits.
PF00644. PARP. 1 hit.
[Graphical view]

SMART

SM00506. A1pp. 2 hits.
[Graphical view]

PROSITE

PS51154. MACRO. 2 hits.
PS51059. PARP_CATALYTIC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

88552.

SOURCE

Search...

Entry information

Entry name

PAR15_HUMAN

Accession

Primary (citable) accession number: Q460N3
Secondary accession number(s): Q8N1K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.