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Q46085

- Q46085_CLOHI

UniProt

Q46085 - Q46085_CLOHI

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Protein
Submitted name: ColH protein
Gene
colH
Organism
Clostridium histolyticum
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi421 – 4211ZincImported
Metal bindingi430 – 4301Calcium 1Imported
Metal bindingi455 – 4551Zinc; via tele nitrogenImported
Metal bindingi459 – 4591Zinc; via tele nitrogenImported
Metal bindingi463 – 4631Calcium 1; via carbonyl oxygenImported
Metal bindingi467 – 4671Calcium 1; via carbonyl oxygenImported
Metal bindingi469 – 4691Calcium 1; via carbonyl oxygenImported
Metal bindingi487 – 4871ZincImported
Metal bindingi814 – 8141Calcium 2Imported
Metal bindingi815 – 8151Calcium 2; via carbonyl oxygenImported
Metal bindingi842 – 8421Calcium 2Imported
Metal bindingi844 – 8441Calcium 2Imported
Metal bindingi884 – 8841Calcium 2Imported
Metal bindingi908 – 9081Cadmium
Metal bindingi908 – 9081Calcium 3Imported
Metal bindingi910 – 9101Cadmium
Metal bindingi910 – 9101Calcium 3Imported
Metal bindingi910 – 9101Calcium 4Imported
Metal bindingi912 – 9121Calcium 4Imported
Metal bindingi913 – 9131Calcium 4Imported
Metal bindingi931 – 9311Cadmium; via carbonyl oxygen
Metal bindingi931 – 9311Calcium 3; via carbonyl oxygenImported
Metal bindingi937 – 9371Cadmium
Metal bindingi937 – 9371Calcium 3Imported
Metal bindingi937 – 9371Calcium 4Imported
Metal bindingi938 – 9381Calcium 4; via carbonyl oxygenImported
Metal bindingi939 – 9391Cadmium
Metal bindingi939 – 9391Calcium 3Imported
Metal bindingi939 – 9391Calcium 4Imported

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Ligandi

    CadmiumImported, CalciumImported, Metal-bindingImported, ZincImported

    Protein family/group databases

    MEROPSiM09.003.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    ColH proteinImported
    Submitted name:
    CollagenaseImported
    Gene namesi
    Name:colHImported
    OrganismiClostridium histolyticumImported
    Taxonomic identifieri1498 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

    Subcellular locationi

    GO - Cellular componenti

    1. collagen trimer Source: UniProtKB-KW
    2. extracellular region Source: InterPro
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4040 PredictedImported
    Add
    BLAST
    Chaini41 – 1021981collagenaseImported
    PRO_5000139769Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3JQWX-ray2.00A/B/C902-1021[»]
    3JQXX-ray2.20A/B/C902-1021[»]
    4AR1X-ray2.01A331-721[»]
    4ARFX-ray1.77A331-721[»]
    4JGUX-ray1.42A/B806-900[»]

    Miscellaneous databases

    EvolutionaryTraceiQ46085.

    Family & Domainsi

    Keywords - Domaini

    CollagenImported, SignalImported

    Family and domain databases

    Gene3Di2.60.40.670. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR007280. Peptidase_C_arc/bac.
    IPR013661. Peptidase_M9_N_dom.
    IPR002169. Peptidase_M9A/M9B.
    IPR022409. PKD/Chitinase_dom.
    IPR000601. PKD_dom.
    [Graphical view]
    PfamiPF01752. Peptidase_M9. 1 hit.
    PF08453. Peptidase_M9_N. 1 hit.
    PF00801. PKD. 2 hits.
    PF04151. PPC. 1 hit.
    [Graphical view]
    PRINTSiPR00931. MICOLLPTASE.
    SMARTiSM00089. PKD. 2 hits.
    [Graphical view]
    SUPFAMiSSF49299. SSF49299. 2 hits.
    SSF49899. SSF49899. 1 hit.
    PROSITEiPS50093. PKD. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q46085-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRKCLSKRL MLAITMATIF TVNSTLPIYA AVDKNNATAA VQNESKRYTV     50
    SYLKTLNYYD LVDLLVKTEI ENLPDLFQYS SDAKEFYGNK TRMSFIMDEI 100
    GRRAPQYTEI DHKGIPTLVE VVRAGFYLGF HNKELNEINK RSFKERVIPS 150
    ILAIQKNPNF KLGTEVQDKI VSATGLLAGN ETAPPEVVNN FTPILQDCIK 200
    NIDRYALDDL KSKALFNVLA APTYDITEYL RATKEKPENT PWYGKIDGFI 250
    NELKKLALYG KINDNNSWII DNGIYHIAPL GKLHSNNKIG IETLTEVMKV 300
    YPYLSMQHLQ SADQIKRHYD SKDAEGNKIP LDKFKKEGKE KYCPKTYTFD 350
    DGKVIIKAGA RVEEEKVKRL YWASKEVNSQ FFRVYGIDKP LEEGNPDDIL 400
    TMVIYNSPEE YKLNSVLYGY DTNNGGMYIE PEGTFFTYER EAQESTYTLE 450
    ELFRHEYTHY LQGRYAVPGQ WGRTKLYDND RLTWYEEGGA ELFAGSTRTS 500
    GILPRKSIVS NIHNTTRNNR YKLSDTVHSK YGASFEFYNY ACMFMDYMYN 550
    KDMGILNKLN DLAKNNDVDG YDNYIRDLSS NYALNDKYQD HMQERIDNYE 600
    NLTVPFVADD YLVRHAYKNP NEIYSEISEV AKLKDAKSEV KKSQYFSTFT 650
    LRGSYTGGAS KGKLEDQKAM NKFIDDSLKK LDTYSWSGYK TLTAYFTNYK 700
    VDSSNRVTYD VVFHGYLPNE GDSKNSLPYG KINGTYKGTE KEKIKFSSEG 750
    SFDPDGKIVS YEWDFGDGNK SNEENPEHSY DKVGTYTVKL KVTDDKGESS 800
    VSTTTAEIKD LSENKLPVIY MHVPKSGALN QKVVFYGKGT YDPDGSIAGY 850
    QWDFGDGSDF SSEQNPSHVY TKKGEYTVTL RVMDSSGQMS EKTMKIKITD 900
    PVYPIGTEKE PNNSKETASG PIVPGIPVSG TIENTSDQDY FYFDVITPGE 950
    VKIDINKLGY GGATWVVYDE NNNAVSYATD DGQNLSGKFK ADKPGRYYIH 1000
    LYMFNGSYMP YRINIEGSVG R 1021
    Length:1,021
    Mass (Da):116,377
    Last modified:November 1, 1996 - v1
    Checksum:i826F45EFD96F917C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D29981 Genomic DNA. Translation: BAA06251.1.
    AB014075 Genomic DNA. Translation: BAA34542.1.
    PIRiI40805.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D29981 Genomic DNA. Translation: BAA06251.1 .
    AB014075 Genomic DNA. Translation: BAA34542.1 .
    PIRi I40805.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3JQW X-ray 2.00 A/B/C 902-1021 [» ]
    3JQX X-ray 2.20 A/B/C 902-1021 [» ]
    4AR1 X-ray 2.01 A 331-721 [» ]
    4ARF X-ray 1.77 A 331-721 [» ]
    4JGU X-ray 1.42 A/B 806-900 [» ]
    ModBasei Search...

    Protein family/group databases

    MEROPSi M09.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q46085.

    Family and domain databases

    Gene3Di 2.60.40.670. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR007280. Peptidase_C_arc/bac.
    IPR013661. Peptidase_M9_N_dom.
    IPR002169. Peptidase_M9A/M9B.
    IPR022409. PKD/Chitinase_dom.
    IPR000601. PKD_dom.
    [Graphical view ]
    Pfami PF01752. Peptidase_M9. 1 hit.
    PF08453. Peptidase_M9_N. 1 hit.
    PF00801. PKD. 2 hits.
    PF04151. PPC. 1 hit.
    [Graphical view ]
    PRINTSi PR00931. MICOLLPTASE.
    SMARTi SM00089. PKD. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49299. SSF49299. 2 hits.
    SSF49899. SSF49899. 1 hit.
    PROSITEi PS50093. PKD. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase."
      Yoshihara K., Matsushita O., Minami J., Okabe A.
      J. Bacteriol. 176:6489-6496(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: JCM 1403Imported.
    2. "Gene duplication and multiplicity of collagenases in Clostridium histolyticum."
      Matsushita O., Jung C.-M., Katayama S., Minami J., Takahashi Y., Okabe A.
      J. Bacteriol. 181:923-933(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: JCM 1403Imported.
    3. "Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum."
      Bauer R., Wilson J.J., Philominathan S.T., Davis D., Matsushita O., Sakon J.
      J. Bacteriol. 195:318-327(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 902-1021 IN COMPLEX WITH CADMIUM AND CALCIUM.
    4. "Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T."
      Eckhard U., Schonauer E., Brandstetter H.
      J. Biol. Chem. 288:20184-20194(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 331-721 IN COMPLEX WITH CALCIUM AND ZINC.
    5. "Crystal structure of Clostridium histolyticum colH collagenase polycystic kidney disease-like domain 2b at 1.4 Angstrom resolution in presence of calcium."
      Bauer R., Matsushita O., Sakon J.
      Submitted (MAR-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 806-900 IN COMPLEX WITH CALCIUM.

    Entry informationi

    Entry nameiQ46085_CLOHI
    AccessioniPrimary (citable) accession number: Q46085
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: July 9, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3

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