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Q46085 (Q46085_CLOHI) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names

Collagenase EMBL BAA06251.1
Gene names
Name:colH EMBL BAA06251.1
OrganismClostridium histolyticum EMBL BAA06251.1
Taxonomic identifier1498 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length1021 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4040 Potential EMBL BAA06251.1
Chain41 – 1021981collagenase EMBL BAA06251.1
PRO_5000139769

Sites

Metal binding4211Zinc
Metal binding4301Calcium 1
Metal binding4551Zinc; via tele nitrogen
Metal binding4591Zinc; via tele nitrogen
Metal binding4631Calcium 1; via carbonyl oxygen
Metal binding4671Calcium 1; via carbonyl oxygen
Metal binding4691Calcium 1; via carbonyl oxygen
Metal binding4871Zinc
Metal binding9081Cadmium PDB 3JQX
Metal binding9081Calcium 2 PDB 3JQW
Metal binding9101Cadmium PDB 3JQX
Metal binding9101Calcium 2 PDB 3JQW
Metal binding9101Calcium 3 PDB 3JQX PDB 3JQW
Metal binding9121Calcium 3 PDB 3JQX PDB 3JQW
Metal binding9131Calcium 3 PDB 3JQX PDB 3JQW
Metal binding9311Cadmium; via carbonyl oxygen PDB 3JQX
Metal binding9311Calcium 2; via carbonyl oxygen PDB 3JQW
Metal binding9371Cadmium PDB 3JQX
Metal binding9371Calcium 2 PDB 3JQW
Metal binding9371Calcium 3 PDB 3JQX PDB 3JQW
Metal binding9381Calcium 3; via carbonyl oxygen PDB 3JQX PDB 3JQW
Metal binding9391Cadmium PDB 3JQX
Metal binding9391Calcium 2 PDB 3JQW
Metal binding9391Calcium 3 PDB 3JQX PDB 3JQW

Sequences

Sequence LengthMass (Da)Tools
Q46085 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 826F45EFD96F917C

FASTA1,021116,377
        10         20         30         40         50         60 
MKRKCLSKRL MLAITMATIF TVNSTLPIYA AVDKNNATAA VQNESKRYTV SYLKTLNYYD 

        70         80         90        100        110        120 
LVDLLVKTEI ENLPDLFQYS SDAKEFYGNK TRMSFIMDEI GRRAPQYTEI DHKGIPTLVE 

       130        140        150        160        170        180 
VVRAGFYLGF HNKELNEINK RSFKERVIPS ILAIQKNPNF KLGTEVQDKI VSATGLLAGN 

       190        200        210        220        230        240 
ETAPPEVVNN FTPILQDCIK NIDRYALDDL KSKALFNVLA APTYDITEYL RATKEKPENT 

       250        260        270        280        290        300 
PWYGKIDGFI NELKKLALYG KINDNNSWII DNGIYHIAPL GKLHSNNKIG IETLTEVMKV 

       310        320        330        340        350        360 
YPYLSMQHLQ SADQIKRHYD SKDAEGNKIP LDKFKKEGKE KYCPKTYTFD DGKVIIKAGA 

       370        380        390        400        410        420 
RVEEEKVKRL YWASKEVNSQ FFRVYGIDKP LEEGNPDDIL TMVIYNSPEE YKLNSVLYGY 

       430        440        450        460        470        480 
DTNNGGMYIE PEGTFFTYER EAQESTYTLE ELFRHEYTHY LQGRYAVPGQ WGRTKLYDND 

       490        500        510        520        530        540 
RLTWYEEGGA ELFAGSTRTS GILPRKSIVS NIHNTTRNNR YKLSDTVHSK YGASFEFYNY 

       550        560        570        580        590        600 
ACMFMDYMYN KDMGILNKLN DLAKNNDVDG YDNYIRDLSS NYALNDKYQD HMQERIDNYE 

       610        620        630        640        650        660 
NLTVPFVADD YLVRHAYKNP NEIYSEISEV AKLKDAKSEV KKSQYFSTFT LRGSYTGGAS 

       670        680        690        700        710        720 
KGKLEDQKAM NKFIDDSLKK LDTYSWSGYK TLTAYFTNYK VDSSNRVTYD VVFHGYLPNE 

       730        740        750        760        770        780 
GDSKNSLPYG KINGTYKGTE KEKIKFSSEG SFDPDGKIVS YEWDFGDGNK SNEENPEHSY 

       790        800        810        820        830        840 
DKVGTYTVKL KVTDDKGESS VSTTTAEIKD LSENKLPVIY MHVPKSGALN QKVVFYGKGT 

       850        860        870        880        890        900 
YDPDGSIAGY QWDFGDGSDF SSEQNPSHVY TKKGEYTVTL RVMDSSGQMS EKTMKIKITD 

       910        920        930        940        950        960 
PVYPIGTEKE PNNSKETASG PIVPGIPVSG TIENTSDQDY FYFDVITPGE VKIDINKLGY 

       970        980        990       1000       1010       1020 
GGATWVVYDE NNNAVSYATD DGQNLSGKFK ADKPGRYYIH LYMFNGSYMP YRINIEGSVG 


R 

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References

[1]"Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase."
Yoshihara K., Matsushita O., Minami J., Okabe A.
J. Bacteriol. 176:6489-6496(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: JCM 1403 EMBL BAA06251.1.
[2]"Gene duplication and multiplicity of collagenases in Clostridium histolyticum."
Matsushita O., Jung C.-M., Katayama S., Minami J., Takahashi Y., Okabe A.
J. Bacteriol. 181:923-933(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: JCM 1403 EMBL BAA34542.1.
[3]"Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum."
Bauer R., Wilson J.J., Philominathan S.T., Davis D., Matsushita O., Sakon J.
J. Bacteriol. 195:318-327(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 902-1021 IN COMPLEX WITH CADMIUM AND CALCIUM.
[4]"Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T."
Eckhard U., Schonauer E., Brandstetter H.
J. Biol. Chem. 288:20184-20194(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 331-721 IN COMPLEX WITH CALCIUM AND ZINC.
[5]"Crystal structure of Clostridium histolyticum colH collagenase polycystic kidney disease-like domain 2b at 1.4 Angstrom resolution in presence of calcium."
Bauer R., Matsushita O., Sakon J.
Submitted (MAR-2013) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 806-900.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D29981 Genomic DNA. Translation: BAA06251.1.
AB014075 Genomic DNA. Translation: BAA34542.1.
PIRI40805.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JQWX-ray2.00A/B/C902-1021[»]
3JQXX-ray2.20A/B/C902-1021[»]
4AR1X-ray2.01A331-721[»]
4ARFX-ray1.77A331-721[»]
4JGUX-ray1.42A/B806-900[»]
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM09.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.670. 2 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR007280. Peptidase_C_arc/bac.
IPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 2 hits.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSPR00931. MICOLLPTASE.
SMARTSM00089. PKD. 2 hits.
[Graphical view]
SUPFAMSSF49299. SSF49299. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEPS50093. PKD. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ46085.

Entry information

Entry nameQ46085_CLOHI
AccessionPrimary (citable) accession number: Q46085
Entry history
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)