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Q46078

- KPYK_CORGL

UniProt

Q46078 - KPYK_CORGL

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Protein

Pyruvate kinase

Gene

pyk

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Metal bindingi35 – 351PotassiumBy similarity
Metal bindingi37 – 371PotassiumBy similarity
Metal bindingi67 – 671PotassiumBy similarity
Sitei218 – 2181Transition state stabilizerBy similarity
Metal bindingi220 – 2201MagnesiumBy similarity
Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
Metal bindingi244 – 2441MagnesiumBy similarity
Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
Binding sitei276 – 2761SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ46078.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:Cgl2089, cg2291
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Pyruvate kinasePRO_0000112068Add
BLAST

2D gel databases

World-2DPAGE0001:Q46078.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi196627.cg2291.

Structurei

3D structure databases

ProteinModelPortaliQ46078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTPTYSGR.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46078-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRRTKIVCT LGPAVASADG ILRLVEDGMD VARLNFSHGD HPDHEQNYKW
60 70 80 90 100
VREAAEKTGR AVGILADLQG PKIRLGRFTD GATVWENGET IRITVDDVEG
110 120 130 140 150
THDRVSTTYK NLAKDAKPGD RLLVDDGKVG LVCVSVEGND VICEVVEGGP
160 170 180 190 200
VSNNKGVSLP GMDISVPALS EKDIRDLRFA LKLGVDFIAL SFVRSPADAE
210 220 230 240 250
LVHKIMDEEG RRVPVIAKLE KPEAVTSLEP IVLAFDAVMV ARGDLGVEVP
260 270 280 290 300
LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV
310 320 330 340 350
LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR
360 370 380 390 400
GVISYSARDI AERLNARALV AFTTSGDTAK RVARLHSHLP LLVFTPNEAV
410 420 430 440 450
RSELALTWGA TTFLCPPVSD TDDMMREVDR ALLAMPEYNK GDMMVVVAGS
460 470
PPGVTGNTNM IHVHLLGDDT RIAKL
Length:475
Mass (Da):51,471
Last modified:November 1, 1996 - v1
Checksum:i4E56A16C570A5386
GO

Sequence cautioni

The sequence CAF20425.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27126 Genomic DNA. Translation: AAA56793.1.
BA000036 Genomic DNA. Translation: BAB99482.1.
BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
PIRiI40840.
RefSeqiNP_601288.2. NC_003450.3.
WP_011014873.1. NC_003450.3.
WP_011265872.1. NC_006958.1.
YP_226326.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99482; BAB99482; BAB99482.
CAF20425; CAF20425; cg2291.
GeneIDi1020040.
3344974.
KEGGicgb:cg2291.
cgl:NCgl2008.
PATRICi21496178. VBICorGlu203724_2025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27126 Genomic DNA. Translation: AAA56793.1 .
BA000036 Genomic DNA. Translation: BAB99482.1 .
BX927154 Genomic DNA. Translation: CAF20425.1 . Different initiation.
PIRi I40840.
RefSeqi NP_601288.2. NC_003450.3.
WP_011014873.1. NC_003450.3.
WP_011265872.1. NC_006958.1.
YP_226326.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q46078.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2291.

2D gel databases

World-2DPAGE 0001:Q46078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99482 ; BAB99482 ; BAB99482 .
CAF20425 ; CAF20425 ; cg2291 .
GeneIDi 1020040.
3344974.
KEGGi cgb:cg2291.
cgl:NCgl2008.
PATRICi 21496178. VBICorGlu203724_2025.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021558.
KOi K00873.
OMAi TPTYSGR.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK Q46078.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum."
    Jetten M.S., Gubler M.E., Lee S.H., Sinskey A.J.
    Appl. Environ. Microbiol. 60:2501-2507(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiKPYK_CORGL
AccessioniPrimary (citable) accession number: Q46078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3