Reviewed, UniProtKB/Swiss-Prot Q46078 (KPYK_CORGL)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	Cgl2089, cg2291

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Pyruvate kinase		PRO_0000112068
Sites
	Active site	218	1	By similarity
	Metal binding	220	1	Magnesium By similarity
	Metal binding	241	1	Magnesium By similarity
	Metal binding	242	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46078-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4E56A16C570A5386	FASTA	475	51,471
	10 20 30 40 50 60 MDRRTKIVCT LGPAVASADG ILRLVEDGMD VARLNFSHGD HPDHEQNYKW VREAAEKTGR 70 80 90 100 110 120 AVGILADLQG PKIRLGRFTD GATVWENGET IRITVDDVEG THDRVSTTYK NLAKDAKPGD 130 140 150 160 170 180 RLLVDDGKVG LVCVSVEGND VICEVVEGGP VSNNKGVSLP GMDISVPALS EKDIRDLRFA 190 200 210 220 230 240 LKLGVDFIAL SFVRSPADAE LVHKIMDEEG RRVPVIAKLE KPEAVTSLEP IVLAFDAVMV 250 260 270 280 290 300 ARGDLGVEVP LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV 310 320 330 340 350 360 LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR GVISYSARDI 370 380 390 400 410 420 AERLNARALV AFTTSGDTAK RVARLHSHLP LLVFTPNEAV RSELALTWGA TTFLCPPVSD 430 440 450 460 470 TDDMMREVDR ALLAMPEYNK GDMMVVVAGS PPGVTGNTNM IHVHLLGDDT RIAKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum." Jetten M.S., Gubler M.E., Lee S.H., Sinskey A.J. Appl. Environ. Microbiol. 60:2501-2507(1994) [PubMed: 8074528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27126 Genomic DNA. Translation: AAA56793.1. BA000036 Genomic DNA. Translation: BAB99482.1. BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
PIR	I40840.
RefSeq	NP_601288.2. YP_226326.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
2-D gel databases
World-2DPAGE	0001:Q46078.
Proteomic databases
PRIDE	Q46078.
Genome annotation databases
GeneID	1020040. 3344974.
GenomeReviews	Gene locus Cgl2089 in contig BA000036_GR. Gene locus cg2291 in contig BX927147_GR.
KEGG	cgb:cg2291. cgl:NCgl2008.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46078.
OMA	Q46078. ATESGYT.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG2291-MON.
BRENDA	2.7.1.40. 812.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_CORGL

Accession

Primary (citable) accession number: Q46078

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)