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Q46078 (KPYK_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Ordered Locus Names:Cgl2089, cg2291
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence CAF20425.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Pyruvate kinase
PRO_0000112068

Sites

Metal binding351Potassium By similarity
Metal binding371Potassium By similarity
Metal binding671Potassium By similarity
Metal binding2201Magnesium By similarity
Metal binding2441Magnesium By similarity
Binding site331Substrate By similarity
Binding site2431Substrate; via amide nitrogen By similarity
Binding site2441Substrate; via amide nitrogen By similarity
Binding site2761Substrate By similarity
Site2181Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46078 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4E56A16C570A5386

FASTA47551,471
        10         20         30         40         50         60 
MDRRTKIVCT LGPAVASADG ILRLVEDGMD VARLNFSHGD HPDHEQNYKW VREAAEKTGR 

        70         80         90        100        110        120 
AVGILADLQG PKIRLGRFTD GATVWENGET IRITVDDVEG THDRVSTTYK NLAKDAKPGD 

       130        140        150        160        170        180 
RLLVDDGKVG LVCVSVEGND VICEVVEGGP VSNNKGVSLP GMDISVPALS EKDIRDLRFA 

       190        200        210        220        230        240 
LKLGVDFIAL SFVRSPADAE LVHKIMDEEG RRVPVIAKLE KPEAVTSLEP IVLAFDAVMV 

       250        260        270        280        290        300 
ARGDLGVEVP LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV 

       310        320        330        340        350        360 
LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR GVISYSARDI 

       370        380        390        400        410        420 
AERLNARALV AFTTSGDTAK RVARLHSHLP LLVFTPNEAV RSELALTWGA TTFLCPPVSD 

       430        440        450        460        470 
TDDMMREVDR ALLAMPEYNK GDMMVVVAGS PPGVTGNTNM IHVHLLGDDT RIAKL 

« Hide

References

« Hide 'large scale' references
[1]"Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum."
Jetten M.S., Gubler M.E., Lee S.H., Sinskey A.J.
Appl. Environ. Microbiol. 60:2501-2507(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27126 Genomic DNA. Translation: AAA56793.1.
BA000036 Genomic DNA. Translation: BAB99482.1.
BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
PIRI40840.
RefSeqNP_601288.2. NC_003450.3.
YP_226326.1. NC_006958.1.

3D structure databases

ProteinModelPortalQ46078.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg2291.

2D gel databases

World-2DPAGE0001:Q46078.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99482; BAB99482; BAB99482.
CAF20425; CAF20425; cg2291.
GeneID1020040.
KEGGcgb:cg2291.
cgl:NCgl2008.
PATRIC21496178. VBICorGlu203724_2025.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021558.
KOK00873.
OMATPTYSGR.
OrthoDBEOG6GBMB0.

Enzyme and pathway databases

SABIO-RKQ46078.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_CORGL
AccessionPrimary (citable) accession number: Q46078
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways