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Protein

Pyruvate kinase

Gene

pyk

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Metal bindingi35 – 351PotassiumBy similarity
Metal bindingi37 – 371PotassiumBy similarity
Metal bindingi67 – 671PotassiumBy similarity
Sitei218 – 2181Transition state stabilizerBy similarity
Metal bindingi220 – 2201MagnesiumBy similarity
Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
Metal bindingi244 – 2441MagnesiumBy similarity
Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
Binding sitei276 – 2761SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ46078.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:Cgl2089, cg2291
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Pyruvate kinasePRO_0000112068Add
BLAST

2D gel databases

World-2DPAGE0001:Q46078.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi196627.cg2291.

Structurei

3D structure databases

ProteinModelPortaliQ46078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiKIVAYCD.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRRTKIVCT LGPAVASADG ILRLVEDGMD VARLNFSHGD HPDHEQNYKW
60 70 80 90 100
VREAAEKTGR AVGILADLQG PKIRLGRFTD GATVWENGET IRITVDDVEG
110 120 130 140 150
THDRVSTTYK NLAKDAKPGD RLLVDDGKVG LVCVSVEGND VICEVVEGGP
160 170 180 190 200
VSNNKGVSLP GMDISVPALS EKDIRDLRFA LKLGVDFIAL SFVRSPADAE
210 220 230 240 250
LVHKIMDEEG RRVPVIAKLE KPEAVTSLEP IVLAFDAVMV ARGDLGVEVP
260 270 280 290 300
LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV
310 320 330 340 350
LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR
360 370 380 390 400
GVISYSARDI AERLNARALV AFTTSGDTAK RVARLHSHLP LLVFTPNEAV
410 420 430 440 450
RSELALTWGA TTFLCPPVSD TDDMMREVDR ALLAMPEYNK GDMMVVVAGS
460 470
PPGVTGNTNM IHVHLLGDDT RIAKL
Length:475
Mass (Da):51,471
Last modified:November 1, 1996 - v1
Checksum:i4E56A16C570A5386
GO

Sequence cautioni

The sequence CAF20425.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27126 Genomic DNA. Translation: AAA56793.1.
BA000036 Genomic DNA. Translation: BAB99482.1.
BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
PIRiI40840.
RefSeqiNP_601288.2. NC_003450.3.
WP_011014873.1. NC_003450.3.
WP_011265872.1. NC_006958.1.
YP_226326.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99482; BAB99482; BAB99482.
CAF20425; CAF20425; cg2291.
GeneIDi1020040.
23503025.
KEGGicgb:cg2291.
cgl:NCgl2008.
PATRICi21496178. VBICorGlu203724_2025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27126 Genomic DNA. Translation: AAA56793.1.
BA000036 Genomic DNA. Translation: BAB99482.1.
BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
PIRiI40840.
RefSeqiNP_601288.2. NC_003450.3.
WP_011014873.1. NC_003450.3.
WP_011265872.1. NC_006958.1.
YP_226326.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ46078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2291.

2D gel databases

World-2DPAGE0001:Q46078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99482; BAB99482; BAB99482.
CAF20425; CAF20425; cg2291.
GeneIDi1020040.
23503025.
KEGGicgb:cg2291.
cgl:NCgl2008.
PATRICi21496178. VBICorGlu203724_2025.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiKIVAYCD.
OrthoDBiEOG6GBMB0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
SABIO-RKQ46078.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum."
    Jetten M.S., Gubler M.E., Lee S.H., Sinskey A.J.
    Appl. Environ. Microbiol. 60:2501-2507(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiKPYK_CORGL
AccessioniPrimary (citable) accession number: Q46078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.