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Q46078

- KPYK_CORGL

UniProt

Q46078 - KPYK_CORGL

Protein

Pyruvate kinase

Gene

pyk

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331SubstrateBy similarity
    Metal bindingi35 – 351PotassiumBy similarity
    Metal bindingi37 – 371PotassiumBy similarity
    Metal bindingi67 – 671PotassiumBy similarity
    Sitei218 – 2181Transition state stabilizerBy similarity
    Metal bindingi220 – 2201MagnesiumBy similarity
    Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
    Metal bindingi244 – 2441MagnesiumBy similarity
    Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
    Binding sitei276 – 2761SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKQ46078.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:Cgl2089, cg2291
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475Pyruvate kinasePRO_0000112068Add
    BLAST

    2D gel databases

    World-2DPAGE0001:Q46078.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi196627.cg2291.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46078.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiTPTYSGR.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46078-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRRTKIVCT LGPAVASADG ILRLVEDGMD VARLNFSHGD HPDHEQNYKW    50
    VREAAEKTGR AVGILADLQG PKIRLGRFTD GATVWENGET IRITVDDVEG 100
    THDRVSTTYK NLAKDAKPGD RLLVDDGKVG LVCVSVEGND VICEVVEGGP 150
    VSNNKGVSLP GMDISVPALS EKDIRDLRFA LKLGVDFIAL SFVRSPADAE 200
    LVHKIMDEEG RRVPVIAKLE KPEAVTSLEP IVLAFDAVMV ARGDLGVEVP 250
    LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV 300
    LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR 350
    GVISYSARDI AERLNARALV AFTTSGDTAK RVARLHSHLP LLVFTPNEAV 400
    RSELALTWGA TTFLCPPVSD TDDMMREVDR ALLAMPEYNK GDMMVVVAGS 450
    PPGVTGNTNM IHVHLLGDDT RIAKL 475
    Length:475
    Mass (Da):51,471
    Last modified:November 1, 1996 - v1
    Checksum:i4E56A16C570A5386
    GO

    Sequence cautioni

    The sequence CAF20425.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27126 Genomic DNA. Translation: AAA56793.1.
    BA000036 Genomic DNA. Translation: BAB99482.1.
    BX927154 Genomic DNA. Translation: CAF20425.1. Different initiation.
    PIRiI40840.
    RefSeqiNP_601288.2. NC_003450.3.
    WP_011014873.1. NC_003450.3.
    YP_226326.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99482; BAB99482; BAB99482.
    CAF20425; CAF20425; cg2291.
    GeneIDi1020040.
    KEGGicgb:cg2291.
    cgl:NCgl2008.
    PATRICi21496178. VBICorGlu203724_2025.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27126 Genomic DNA. Translation: AAA56793.1 .
    BA000036 Genomic DNA. Translation: BAB99482.1 .
    BX927154 Genomic DNA. Translation: CAF20425.1 . Different initiation.
    PIRi I40840.
    RefSeqi NP_601288.2. NC_003450.3.
    WP_011014873.1. NC_003450.3.
    YP_226326.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q46078.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2291.

    2D gel databases

    World-2DPAGE 0001:Q46078.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99482 ; BAB99482 ; BAB99482 .
    CAF20425 ; CAF20425 ; cg2291 .
    GeneIDi 1020040.
    KEGGi cgb:cg2291.
    cgl:NCgl2008.
    PATRICi 21496178. VBICorGlu203724_2025.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    KOi K00873.
    OMAi TPTYSGR.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK Q46078.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum."
      Jetten M.S., Gubler M.E., Lee S.H., Sinskey A.J.
      Appl. Environ. Microbiol. 60:2501-2507(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiKPYK_CORGL
    AccessioniPrimary (citable) accession number: Q46078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3