PTS system mannose-specific EIIBCA component

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Q46072 (PTN3B_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PTS system mannose-specific EIIBCA component

Alternative name(s):
EII-Man/EIII-Man
EIIBCA-Man

Including the following 3 domains:

Mannose-specific phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system mannose-specific EIIB component
Mannose permease IIC component
Alternative name(s):
PTS system mannose-specific EIIC component
Mannose-specific phosphotransferase enzyme IIA component
EC=2.7.1.-
Alternative name(s):
PTS system mannose-specific EIIA component

Gene names

Name:	ptsM
Synonyms:	ptsG
Ordered Locus Names:	Cgl1360, cg1537

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 683

683

PTS system mannose-specific EIIBCA component

PRO_0000186645

Regions

Transmembrane

126 – 146

Helical; Potential

Transmembrane

162 – 182

Helical; Potential

Transmembrane

193 – 213

Helical; Potential

Transmembrane

225 – 245

Helical; Potential

Transmembrane

260 – 280

Helical; Potential

Transmembrane

303 – 323

Helical; Potential

Transmembrane

344 – 364

Helical; Potential

Transmembrane

376 – 396

Helical; Potential

Transmembrane

409 – 429

Helical; Potential

Transmembrane

442 – 462

Helical; Potential

Domain

1 – 89

PTS EIIB type-1

Domain

117 – 476

360

PTS EIIC type-1

Domain

550 – 654

105

PTS EIIA type-1

Sites

Active site

Phosphocysteine intermediate; for EIIB activity By similarity

Active site

602

Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q46072 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0B42CAEC60828075
Show »

FASTA

683

72,571

        10         20         30         40         50         60 
MASKLTTTSQ HILENLGGPD NITSMTHCAT RLRFQVKDQS IVDQQEIDSD PSVLGVVPQG 

        70         80         90        100        110        120 
STGMQVVMGG SVANYYQEIL KLDGMKHFAD GEATESSSKK EYGGVRGKYS WIDYAFEFLS 

       130        140        150        160        170        180 
DTFRPILWAL LGASLIITLL VLADTFGLQD FRAPMDEQPD TYVFLHSMWR SVFYFLPIMV 

       190        200        210        220        230        240 
GATAARKLGA NEWIGAAIPA ALLTPEFLAL GSAGDTVTVF GLPMVLNDYS GQVFPPLIAA 

       250        260        270        280        290        300 
IGLYWVEKGL KKIIPEAVQM VFVPFFSLLI MIPATAFLLG PFGIGVGNGI SNLLEAINNF 

       310        320        330        340        350        360 
SPFILSIVIP LLYPFLVPLG LHWPLNAIMI QNINTLGYDF IQGPMGAWNF ACFGLVTGVF 

       370        380        390        400        410        420 
LLSIKERNKA MRQVSLGGML AGLLGGISEP SLYGVLLRFK KTYFRLLPGC LAGGIVMGIF 

       430        440        450        460        470        480 
DIKAYAFVFT SLLTIPAMDP WLGYTIGIAV AFFVSMFLVL ALDYRSNEER DEARAKVAAD 

       490        500        510        520        530        540 
KQAEEDLKAE ANATPAAPVA AAGAGAGAGA GAAAGAATAV AAKPKLAAGE VVDIVSPLEG 

       550        560        570        580        590        600 
KAIPLSEVPD PIFAAGKLGP GIAIQPTGNT VVAPADATVI LVQKSGHAVA LRLDSGVEIL 

       610        620        630        640        650        660 
VHVGLDTVQL GGEGFTVHVE RRQQVKAGDP LITFDADFIR SKDLPLITPV VVSNAAKFGE 

       670        680 
IEGIPADQAN SSTTVIKVNG KNE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II and analyses of the deduced protein sequence." Lee J.K., Sung M.H., Yoon K.H., Yu J.H., Oh T.K. FEMS Microbiol. Lett. 119:137-145(1994) [PubMed: 8039653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L18874 Genomic DNA. Translation: AAA53546.1. BA000036 Genomic DNA. Translation: BAB98753.1. BX927152 Genomic DNA. Translation: CAF21369.1.
RefSeq	NP_600576.1. NC_003450.3. YP_225645.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q46072.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019335. 3344282.
GenomeReviews	Gene locus Cgl1360 in contig BA000036_GR. Gene locus cg1537 in contig BX927147_GR.
KEGG	cgb:cg1537. cgl:NCgl1305.
PATRIC	21494755. VBICorGlu203724_1327.
Phylogenomic databases
HOGENOM	HBG673521.
OMA	ANEWIGA.
PhylomeDB	Q46072.
ProtClustDB	CLSK633359.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1537-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
KO	K02755. K02756. K02757.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTN3B_CORGL

Accession

Primary (citable) accession number: Q46072

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 3 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents