Reviewed, UniProtKB/Swiss-Prot Q46072 (PTN3B_CORGL)

Names and origin

Protein names

Recommended name:
    PTS system mannose-specific EIIBCA component
Alternative name(s):
    EIIBCA-Man
    EII-Man/EIII-Man
Including the following 3 domains:
    1- Recommended name:
            Mannose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannose-specific EIIB component
    2- Recommended name:
            Mannose permease IIC component
        Alternative name(s):
            PTS system mannose-specific EIIC component
    3- Recommended name:
            Mannose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannose-specific EIIA component

Gene names

Name:	ptsM
Synonyms:	ptsG
Ordered Locus Names:	Cgl1360, cg1537

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	PTS system mannose-specific EIIBCA component		PRO_0000186645
Regions
	Transmembrane	126 – 146	21	Potential
	Transmembrane	162 – 182	21	Potential
	Transmembrane	193 – 213	21	Potential
	Transmembrane	225 – 245	21	Potential
	Transmembrane	260 – 280	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	344 – 364	21	Potential
	Transmembrane	376 – 396	21	Potential
	Transmembrane	409 – 429	21	Potential
	Transmembrane	442 – 462	21	Potential
	Domain	1 – 89	89	PTS EIIB type-1
	Domain	117 – 476	360	PTS EIIC type-1
	Domain	550 – 654	105	PTS EIIA type-1
Sites
	Active site	28	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	602	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46072-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0B42CAEC60828075	FASTA	683	72,571
	10 20 30 40 50 60 MASKLTTTSQ HILENLGGPD NITSMTHCAT RLRFQVKDQS IVDQQEIDSD PSVLGVVPQG 70 80 90 100 110 120 STGMQVVMGG SVANYYQEIL KLDGMKHFAD GEATESSSKK EYGGVRGKYS WIDYAFEFLS 130 140 150 160 170 180 DTFRPILWAL LGASLIITLL VLADTFGLQD FRAPMDEQPD TYVFLHSMWR SVFYFLPIMV 190 200 210 220 230 240 GATAARKLGA NEWIGAAIPA ALLTPEFLAL GSAGDTVTVF GLPMVLNDYS GQVFPPLIAA 250 260 270 280 290 300 IGLYWVEKGL KKIIPEAVQM VFVPFFSLLI MIPATAFLLG PFGIGVGNGI SNLLEAINNF 310 320 330 340 350 360 SPFILSIVIP LLYPFLVPLG LHWPLNAIMI QNINTLGYDF IQGPMGAWNF ACFGLVTGVF 370 380 390 400 410 420 LLSIKERNKA MRQVSLGGML AGLLGGISEP SLYGVLLRFK KTYFRLLPGC LAGGIVMGIF 430 440 450 460 470 480 DIKAYAFVFT SLLTIPAMDP WLGYTIGIAV AFFVSMFLVL ALDYRSNEER DEARAKVAAD 490 500 510 520 530 540 KQAEEDLKAE ANATPAAPVA AAGAGAGAGA GAAAGAATAV AAKPKLAAGE VVDIVSPLEG 550 560 570 580 590 600 KAIPLSEVPD PIFAAGKLGP GIAIQPTGNT VVAPADATVI LVQKSGHAVA LRLDSGVEIL 610 620 630 640 650 660 VHVGLDTVQL GGEGFTVHVE RRQQVKAGDP LITFDADFIR SKDLPLITPV VVSNAAKFGE 670 680 IEGIPADQAN SSTTVIKVNG KNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II and analyses of the deduced protein sequence." Lee J.K., Sung M.H., Yoon K.H., Yu J.H., Oh T.K. FEMS Microbiol. Lett. 119:137-145(1994) [PubMed: 8039653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18874 Genomic DNA. Translation: AAA53546.1. BA000036 Genomic DNA. Translation: BAB98753.1. BX927152 Genomic DNA. Translation: CAF21369.1.
RefSeq	NP_600576.1. YP_225645.1.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P08837.
ModBase	Search...
Genome annotation databases
GeneID	1019335. 3344282.
GenomeReviews	Gene locus Cgl1360 in contig BA000036_GR. Gene locus cg1537 in contig BX927147_GR.
KEGG	cgb:cg1537. cgl:NCgl1305.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46072.
OMA	Q46072. IMVPLTV.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1537-MON.
BRENDA	2.7.1.69. 812.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phospho_site. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
ProDom	PD001476. Ptrans_EIIB. 1 hit. PD002243. PTS_EIIA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTN3B_CORGL

Accession

Primary (citable) accession number: Q46072

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)