ID   MBHL_CITFR              Reviewed;         597 AA.
AC   Q46046;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=Hydrogenase-1 large chain;
DE            Short=HYD1;
DE            EC=1.12.99.6;
DE   AltName: Full=Membrane-bound hydrogenase 1 large subunit;
DE   AltName: Full=NiFe hydrogenase;
GN   Name=hyaB;
OS   Citrobacter freundii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Yano K., Ikebukuro K., Tomiyama M., Karube I.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family.
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DR   EMBL; D28594; BAA05930.1; -; Genomic_DNA.
DR   HSSP; P18188; 1FRF.
DR   BRENDA; 1.12.99.6; 2347.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0016151; F:nickel ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.
FT   CHAIN         1    597       Hydrogenase-1 large chain.
FT                                /FTId=PRO_0000199711.
FT   METAL        76     76       Nickel (Potential).
FT   METAL        79     79       Nickel (Potential).
FT   METAL       576    576       Nickel (Potential).
FT   METAL       579    579       Nickel (Potential).
SQ   SEQUENCE   597 AA;  66391 MW;  F9CCABD815D017F4 CRC64;
     MSNSYQTQGY TVNNAGRRLV VDPITRIEGH MRCEVNIDEQ NIITNAVSCG TMFRGLEIIL
     QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP DNANIIRNIM LATLWCHDHL
     VHFYQLAGMD WIDVLNALKA DPRATSQLAQ SLSAWPMSSP GYFFDVQNRL KKFVDGGQLG
     IFRNGYWGHP QYKLSPEANL MGFAHYLEAL DFQREIIKIH TVFGGKNPHP NWIVGGMPCA
     INIDQSGAVG AVDMERLNLV QSIITRTADF INNVMVPDAL AIGQFNKPWS QIGTGLSDKC
     VLSYGAFPDI ANDFSAKSLL MPGGAVINGD FNNVMPVDLA DQQQIQEFVD HAWYRYPDDQ
     LGRHPFEGIT EPWYNPGDVK GSDTDIQQLN EQERYSWIKA PRWRGHAMEV GPLARTLIAY
     HKGDAATIES VDRMMSALKL PLSGMQSTLG RILCRAHEAQ WAVSKLQYFF DKLMTNLKNG
     NLATANTEKW EPASWPQQCR GIGFTEAPRG ALGHWASIRD QKIDVYQCVV PTTWNASPRD
     PEGQIGAYEA ALMGTQMAIP DQPLEILRTL HSFDPCLACS THVLGDDGSE LIAVQVR
//