Q46046 (MBHL_CITFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydrogenase-1 large chain
Short name=HYD1
EC=1.12.99.6

Alternative name(s):
Membrane-bound hydrogenase 1 large subunit
NiFe hydrogenase

Gene names

Name:

hyaB

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Hydrogenase-1 large chain		PRO_0000199711
Sites
	Metal binding	76	1	Nickel Potential
	Metal binding	79	1	Nickel Potential
	Metal binding	576	1	Nickel Potential
	Metal binding	579	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q46046 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F9CCABD815D017F4	FASTA	597	66,391
	10 20 30 40 50 60 MSNSYQTQGY TVNNAGRRLV VDPITRIEGH MRCEVNIDEQ NIITNAVSCG TMFRGLEIIL 70 80 90 100 110 120 QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP DNANIIRNIM LATLWCHDHL 130 140 150 160 170 180 VHFYQLAGMD WIDVLNALKA DPRATSQLAQ SLSAWPMSSP GYFFDVQNRL KKFVDGGQLG 190 200 210 220 230 240 IFRNGYWGHP QYKLSPEANL MGFAHYLEAL DFQREIIKIH TVFGGKNPHP NWIVGGMPCA 250 260 270 280 290 300 INIDQSGAVG AVDMERLNLV QSIITRTADF INNVMVPDAL AIGQFNKPWS QIGTGLSDKC 310 320 330 340 350 360 VLSYGAFPDI ANDFSAKSLL MPGGAVINGD FNNVMPVDLA DQQQIQEFVD HAWYRYPDDQ 370 380 390 400 410 420 LGRHPFEGIT EPWYNPGDVK GSDTDIQQLN EQERYSWIKA PRWRGHAMEV GPLARTLIAY 430 440 450 460 470 480 HKGDAATIES VDRMMSALKL PLSGMQSTLG RILCRAHEAQ WAVSKLQYFF DKLMTNLKNG 490 500 510 520 530 540 NLATANTEKW EPASWPQQCR GIGFTEAPRG ALGHWASIRD QKIDVYQCVV PTTWNASPRD 550 560 570 580 590 PEGQIGAYEA ALMGTQMAIP DQPLEILRTL HSFDPCLACS THVLGDDGSE LIAVQVR

References

[1]	Yano K., Ikebukuro K., Tomiyama M., Karube I. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28594 Genomic DNA. Translation: BAA05930.1.
3D structure databases
ProteinModelPortal	Q46046.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_CITFR

Accession

Primary (citable) accession number: Q46046

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program