ID   MBHS_CITFR              Reviewed;         375 AA.
AC   Q46045;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Hydrogenase-1 small chain;
DE            Short=HYD1;
DE            EC=1.12.99.6;
DE   AltName: Full=Membrane-bound hydrogenase 1 small subunit;
DE   AltName: Full=NiFe hydrogenase;
DE   Flags: Precursor;
GN   Name=hyaA;
OS   Citrobacter freundii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Yano K., Ikebukuro K., Tomiyama M., Karube I.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC       Periplasmic side.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small
CC       subunit family.
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DR   EMBL; D28594; BAA05929.1; -; Genomic_DNA.
DR   HSSP; P18187; 1FRF.
DR   BRENDA; 1.12.99.6; 2347.
DR   GO; GO:0009375; C:ferredoxin hydrogenase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR   InterPro; IPR001821; NiFe_hydrogenase_ssu.
DR   InterPro; IPR006311; Tat.
DR   InterPro; IPR017909; Twin_arg_translocation_Tat.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   PIRSF; PIRSF000310; NiFe_hyd_ssu; 1.
DR   PRINTS; PR00614; NIHGNASESMLL.
DR   TIGRFAMs; TIGR00391; hydA; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   3Fe-4S; 4Fe-4S; Cell membrane; Iron; Iron-sulfur; Membrane;
KW   Metal-binding; Oxidoreductase; Signal.
FT   SIGNAL        1     47       Tat-type signal (Potential).
FT   CHAIN        48    375       Hydrogenase-1 small chain.
FT                                /FTId=PRO_0000013426.
FT   METAL        64     64       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL        67     67       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       162    162       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       196    196       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       234    234       Iron-sulfur 2 (4Fe-4S); via pros nitrogen
FT                                (By similarity).
FT   METAL       237    237       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       262    262       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       268    268       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       277    277       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       296    296       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       299    299       Iron-sulfur 3 (3Fe-4S) (By similarity).
SQ   SEQUENCE   375 AA;  40923 MW;  0B344C52BEF06E5C CRC64;
     MNTNNEETFY QAMRRKGVSR RSFLKYCSLA ATSLGLGAAM TPRIAWALEN KPRIPVVWIH
     GLECTCCTES FIRSSHPLAK DVILSLISLD YDDTLMAAAG TQAEEVFEDI LSRYHGRYIL
     AVEGNPPLGE QGMFCISGGR PFIEKAKRAA AGASAIIAWG TCASWGCVQA ARPNPTQATP
     IDKVITDKPI VKVPGCPPIP DVMSAIITYM VTFDRLPELD RLGRPLMFYG QRIHDKCYRR
     AHFDAGEFVE SWDDDAARKG YCLYKMGCKG PTTYNACSTT RWNGGVSFPI QSGHGCLGCS
     ENGFWDRGSF YSRVVDIPQM GTHSTADTVG LTALGVVASS VGVHAVASAV NQHNRHKQQL
     ADAGQQSPDN EDKQA
//