Q46045 (MBHS_CITFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydrogenase-1 small chain
Short name=HYD1
EC=1.12.99.6

Alternative name(s):
Membrane-bound hydrogenase 1 small subunit
NiFe hydrogenase

Gene names

Name:

hyaA

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 47	47	Tat-type signal Potential
	Chain	48 – 375	328	Hydrogenase-1 small chain		PRO_0000013426
Sites
	Metal binding	64	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	67	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	234	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	237	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	262	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	268	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	277	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	296	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	299	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46045 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0B344C52BEF06E5C	FASTA	375	40,923
	10 20 30 40 50 60 MNTNNEETFY QAMRRKGVSR RSFLKYCSLA ATSLGLGAAM TPRIAWALEN KPRIPVVWIH 70 80 90 100 110 120 GLECTCCTES FIRSSHPLAK DVILSLISLD YDDTLMAAAG TQAEEVFEDI LSRYHGRYIL 130 140 150 160 170 180 AVEGNPPLGE QGMFCISGGR PFIEKAKRAA AGASAIIAWG TCASWGCVQA ARPNPTQATP 190 200 210 220 230 240 IDKVITDKPI VKVPGCPPIP DVMSAIITYM VTFDRLPELD RLGRPLMFYG QRIHDKCYRR 250 260 270 280 290 300 AHFDAGEFVE SWDDDAARKG YCLYKMGCKG PTTYNACSTT RWNGGVSFPI QSGHGCLGCS 310 320 330 340 350 360 ENGFWDRGSF YSRVVDIPQM GTHSTADTVG LTALGVVASS VGVHAVASAV NQHNRHKQQL 370 ADAGQQSPDN EDKQA

References

[1]	Yano K., Ikebukuro K., Tomiyama M., Karube I. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28594 Genomic DNA. Translation: BAA05929.1.
3D structure databases
ProteinModelPortal	Q46045.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. HydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_CITFR

Accession

Primary (citable) accession number: Q46045

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program