Reviewed, UniProtKB/Swiss-Prot Q45Z30 (PA21_TROCA)

Names and origin

Protein names	Recommended name:     Phospholipase A2, acidic 1     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase 1       Short name=PLA-1
Organism	Tropidechis carinatus (Australian rough-scaled snake)
Taxonomic identifier	100989 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Notechinae › Tropidechis

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 151	124	Phospholipase A2, acidic 1		PRO_0000043276
Sites
	Active site	75	1	By similarity
	Active site	126	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 104		By similarity
	Disulfide bond	54 ↔ 151		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 132		By similarity
	Disulfide bond	78 ↔ 125		By similarity
	Disulfide bond	88 ↔ 118		By similarity
	Disulfide bond	111 ↔ 123		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q45Z30-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 663C0EF2DEB9935A	FASTA	151	16,921
	10 20 30 40 50 60 MYPAHLLVLL AVCVSLLGAA SIPARPLNLY QFGNMIQCAN HGRRPTRHYM DYGCYCGKGG 70 80 90 100 110 120 SGTPVDELDR CCQTHDDCYG EAEKLPACNY MMSGPYYNTY SYECNDGELT CKDNNDECKA 130 140 150 FICNCDRTAA ICFARTPYND ANWNIDTKTR C

References

[1]

"Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species." St Pierre L., Woods R., Earl S.T.H., Masci P.P., Lavin M.F. Cell. Mol. Life Sci. 62:2679-2693(2005) [PubMed: 16261251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ085836 mRNA. Translation: AAZ22654.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q45Z30.
Enzyme and pathway databases
BRENDA	3.1.1.4. 295067.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21_TROCA

Accession

Primary (citable) accession number: Q45Z30

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)