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Q45UG0

- RDRP_ROTJ1

UniProt

Q45UG0 - RDRP_ROTJ1

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifieri335103 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007663: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11671167RNA-directed RNA polymerasePRO_0000369831Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 Reviewed prediction. Interacts with VP2 Reviewed prediction. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 735183RdRp catalyticAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45UG0-1 [UniParc]FASTAAdd to Basket

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MEPENYLAWL ARDIVRNLSY TSLVYNNPKV AIVELLDNKE AFFAYEKEQK     50
TPEALINYID SIVKSSISVE DKIEALLKIR YISVYVDDKS DKRDIVLQLL 100
NRTIKKIELK TKISDELNDA INAITIESKN WKIQNSKSFK PYHYNQLVSD 150
FIKYNEFEVL EGTDPLKWKS DTLQGLSPNY NHRTHTLISS IIYATSVRFD 200
NYNDEQLQVL LYLFSVIRTN YVNGYLEILP NRKWSHSLAD LRENKSIMMY 250
SAKIIHASCA MISILHAVPI DYFFLAQIIA SFSEIPAHAA KHLSSPMTLY 300
IGIAQLRSNI VVSTKIAAES VATESPNISR LEESQIREWE QEMSEYPFQS 350
SRMVRMMKKN IFDVSVDMFY AIFNCFSATF HVGHRIDNPQ DAIEAQVKVE 400
YTSDVDKEMY DQYYFLLKRM LTDQLAEYAE EMYFKYNSDV TAESLAAMAN 450
SSNGYSRSVT FLDREIKTTK KMLHLDDDLS KNLNFTNIGD QIKKGIPMGT 500
RNVPARQTRG IFILSWQVAA IQHTIAEFLY KKAKKGGFGA TFAEAYVAKA 550
ATLTYGILAE ATSKADQLIL YTDVSQWDAS QHNTEPYRSA WINAIKEART 600
KYKINYNQEP VVLGMNVLDK MIEIQEALLN SNLIVESQGS KRQPLRIKYH 650
GVASGEKTTK IGNSFANVAL ITTVFNNLTN TMPSIRVNHM RVDGDDNVVT 700
MYTANRIDEV QENIKEKYKR MNAKVKALAS YTGLEMAKRF IICGKIFERG 750
AISIFTAERP YGTDLSVQST TGSLIYSAAV NAYRGFGDNY LNFMTDVLVP 800
PSASVKITGR LRSLLSPVTL YSTGPLSFEI TPYGLGGRMR LFSLSKENME 850
LYKILTSSLA ISVQPDEIKK YSSTPQFKAR VDRMISSVQI AMKSEAKIIT 900
SILRDKEEQK TLGVPNVATT KNRQQIEKAR KTLSLPKETL PKVTKYYPEE 950
IFHLILRNST FTIPKLNTMT KVYMNNSANI TKLQQQLGVR VSSGIQVHRP 1000
VNTLLKLVEK HSPIKISPSD LVLYSKKYDL TNLNGKKQFL IDLGISGNEL 1050
RFYLNSKLLF HDLLLSKYDK LYESPGFGAT QLNALPLDLT AAEKVFSIKL 1100
NLPNTYYELL MLILLYEYVN FVMFTGDTFR AVCIPESQTI NAKLVKTVMT 1150
MIDNIQLDTV MFSDNIY 1167
Length:1,167
Mass (Da):132,891
Last modified:September 13, 2005 - v1
Checksum:i0582DDA5F8892162
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113897 Genomic RNA. Translation: AAZ03485.1.
RefSeqiYP_392490.1. NC_007548.1.

Genome annotation databases

GeneIDi5076650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113897 Genomic RNA. Translation: AAZ03485.1 .
RefSeqi YP_392490.1. NC_007548.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5076650.

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
    Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
    J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_ROTJ1
AccessioniPrimary (citable) accession number: Q45UG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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