Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q45UG0 (RDRP_ROTJ1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase

EC=2.7.7.48
Alternative name(s):
Protein VP1
OrganismRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19)) [Reference proteome]
Taxonomic identifier335103 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1167 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Interacts with VP3 Potential. Interacts with VP2 Potential. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the reoviridae RNA-directed RNA polymerase family.

Contains 1 RdRp catalytic domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11671167RNA-directed RNA polymerase
PRO_0000369831

Regions

Domain553 – 735183RdRp catalytic

Sequences

Sequence LengthMass (Da)Tools
Q45UG0 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 0582DDA5F8892162

FASTA1,167132,891
        10         20         30         40         50         60 
MEPENYLAWL ARDIVRNLSY TSLVYNNPKV AIVELLDNKE AFFAYEKEQK TPEALINYID 

        70         80         90        100        110        120 
SIVKSSISVE DKIEALLKIR YISVYVDDKS DKRDIVLQLL NRTIKKIELK TKISDELNDA 

       130        140        150        160        170        180 
INAITIESKN WKIQNSKSFK PYHYNQLVSD FIKYNEFEVL EGTDPLKWKS DTLQGLSPNY 

       190        200        210        220        230        240 
NHRTHTLISS IIYATSVRFD NYNDEQLQVL LYLFSVIRTN YVNGYLEILP NRKWSHSLAD 

       250        260        270        280        290        300 
LRENKSIMMY SAKIIHASCA MISILHAVPI DYFFLAQIIA SFSEIPAHAA KHLSSPMTLY 

       310        320        330        340        350        360 
IGIAQLRSNI VVSTKIAAES VATESPNISR LEESQIREWE QEMSEYPFQS SRMVRMMKKN 

       370        380        390        400        410        420 
IFDVSVDMFY AIFNCFSATF HVGHRIDNPQ DAIEAQVKVE YTSDVDKEMY DQYYFLLKRM 

       430        440        450        460        470        480 
LTDQLAEYAE EMYFKYNSDV TAESLAAMAN SSNGYSRSVT FLDREIKTTK KMLHLDDDLS 

       490        500        510        520        530        540 
KNLNFTNIGD QIKKGIPMGT RNVPARQTRG IFILSWQVAA IQHTIAEFLY KKAKKGGFGA 

       550        560        570        580        590        600 
TFAEAYVAKA ATLTYGILAE ATSKADQLIL YTDVSQWDAS QHNTEPYRSA WINAIKEART 

       610        620        630        640        650        660 
KYKINYNQEP VVLGMNVLDK MIEIQEALLN SNLIVESQGS KRQPLRIKYH GVASGEKTTK 

       670        680        690        700        710        720 
IGNSFANVAL ITTVFNNLTN TMPSIRVNHM RVDGDDNVVT MYTANRIDEV QENIKEKYKR 

       730        740        750        760        770        780 
MNAKVKALAS YTGLEMAKRF IICGKIFERG AISIFTAERP YGTDLSVQST TGSLIYSAAV 

       790        800        810        820        830        840 
NAYRGFGDNY LNFMTDVLVP PSASVKITGR LRSLLSPVTL YSTGPLSFEI TPYGLGGRMR 

       850        860        870        880        890        900 
LFSLSKENME LYKILTSSLA ISVQPDEIKK YSSTPQFKAR VDRMISSVQI AMKSEAKIIT 

       910        920        930        940        950        960 
SILRDKEEQK TLGVPNVATT KNRQQIEKAR KTLSLPKETL PKVTKYYPEE IFHLILRNST 

       970        980        990       1000       1010       1020 
FTIPKLNTMT KVYMNNSANI TKLQQQLGVR VSSGIQVHRP VNTLLKLVEK HSPIKISPSD 

      1030       1040       1050       1060       1070       1080 
LVLYSKKYDL TNLNGKKQFL IDLGISGNEL RFYLNSKLLF HDLLLSKYDK LYESPGFGAT 

      1090       1100       1110       1120       1130       1140 
QLNALPLDLT AAEKVFSIKL NLPNTYYELL MLILLYEYVN FVMFTGDTFR AVCIPESQTI 

      1150       1160 
NAKLVKTVMT MIDNIQLDTV MFSDNIY 

« Hide

References

[1]"Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ113897 Genomic RNA. Translation: AAZ03485.1.
RefSeqYP_392490.1. NC_007548.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5076650.

Family and domain databases

InterProIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamPF02123. RdRP_4. 1 hit.
[Graphical view]
PROSITEPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_ROTJ1
AccessionPrimary (citable) accession number: Q45UG0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families