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Q45UF8

- VP4_ROTJ1

UniProt

Q45UF8 - VP4_ROTJ1

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Protein
Outer capsid protein VP4
Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.
VP8* forms the head of the spikes By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei245 – 2462Cleavage By similarity
Sitei259 – 2602Cleavage By similarity

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifieri335103 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007663: Genome

Subcellular locationi

Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein By similarity.
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein By similarity.

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823Outer capsid protein VP4
PRO_0000369845Add
BLAST
Chaini1 – 245245Outer capsid protein VP8* Reviewed prediction
PRO_0000369846Add
BLAST
Chaini260 – 823564Outer capsid protein VP5* Reviewed prediction
PRO_0000369847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi143 – 1431N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi153 – 1531N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi198 – 1981N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi261 – 2611N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi330 – 3301N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi484 – 4841N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi491 – 4911N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi511 – 5111N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi558 – 5581N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi620 – 6201N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi801 – 8011N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer Reviewed prediction.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi256 – 2605Poly-Lys

Sequence similaritiesi

Belongs to the rotavirus VP4 family.

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45UF8-1 [UniParc]FASTAAdd to Basket

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MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RIEKDGEKAY    50
CFKNLDPVRY DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL KADTDIDLSD 100
DVKDDLKRQI MEYYQQGYRA IFLVRPQNQE QHYIASYSST NLNFTSQPSI 150
GVNLSILNKI QENKLYIYST QPHIPSVGCE MIAKIFRTDI DNENSLINYS 200
APVTVTISVT KATFGDTFVC NHPNMNYQDL IPTMTKNSIY HDVKRITKIH 250
EYINSKKKKK NSTSKIGGIQ IAESKDGFWK ILTKNYQIKL KFGVEGYGVM 300
GGTFGNWLID SGFKTVETNY EYQRNGKTIN ATTVASVKPS RKCGTRSPVF 350
GQLQFSGEMM VLSHNDILTV FYTEREWALS NAIYAKNFAT DFKRQFEITA 400
QSDELLVRTN VVPHTIKNTP GKALMEYSHG GFGQIDTSDY TGMALTFRFR 450
CISEDLPEGY YDRDKALAFA NVGLTSFQDR QEANGTYWVY NTSTVGFGSC 500
YPKKEFEYDI NVTYTTLLPS DPEFTTGGTN YAQSVTAVLE ESFINLQNQV 550
NEMLTRMNIS DLTSGVMSVF SVATSFPQIL DGISDLLKAA SSAFKKVKGK 600
VGSVAKRLRG KRYVRLFDEN VSIEETPRFL DSIRSSRRPS ILSNMFNDDE 650
TFTALHTLAS RTNSVASDVT YLQPIITTRI ANSTPPVIAP ASSVTYAKLK 700
DISKIINAEI DPKSIMEFNQ ISNTISILDS TKKLAQYAVD PDIIDGILNK 750
MVGGHARSLF SLKVRKHLLD AVEKDAFVKY NYHDLMGKLL NDRELLDITN 800
NLSSQKQFEL AKEFRDLLIN ALA 823
Length:823
Mass (Da):92,912
Last modified:September 13, 2005 - v1
Checksum:iFD0AA4BA99AEB512
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113899 Genomic RNA. Translation: AAZ03487.1.
RefSeqiYP_392492.1. NC_007550.1.

Genome annotation databases

GeneIDi5076652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ113899 Genomic RNA. Translation: AAZ03487.1 .
RefSeqi YP_392492.1. NC_007550.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5076652.

Family and domain databases

InterProi IPR000416. Haemagglutinin_VP4.
[Graphical view ]
Pfami PF00426. VP4_haemagglut. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
    Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
    J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP4_ROTJ1
AccessioniPrimary (citable) accession number: Q45UF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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