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Q45UF8

- VP4_ROTJ1

UniProt

Q45UF8 - VP4_ROTJ1

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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors (By similarity).By similarity
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm (By similarity).By similarity
VP8* forms the head of the spikes.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei245 – 2462CleavageBy similarity
Sitei259 – 2602CleavageBy similarity

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifieri335103 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007663: Genome

Subcellular locationi

Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein.By similarity
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein.By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823Outer capsid protein VP4PRO_0000369845Add
BLAST
Chaini1 – 245245Outer capsid protein VP8*Sequence AnalysisPRO_0000369846Add
BLAST
Chaini260 – 823564Outer capsid protein VP5*Sequence AnalysisPRO_0000369847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi143 – 1431N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi198 – 1981N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi261 – 2611N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi484 – 4841N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi511 – 5111N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi558 – 5581N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi620 – 6201N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi801 – 8011N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer.Curated

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi256 – 2605Poly-Lys

Sequence similaritiesi

Belongs to the rotavirus VP4 family.Curated

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q45UF8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RIEKDGEKAY
60 70 80 90 100
CFKNLDPVRY DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL KADTDIDLSD
110 120 130 140 150
DVKDDLKRQI MEYYQQGYRA IFLVRPQNQE QHYIASYSST NLNFTSQPSI
160 170 180 190 200
GVNLSILNKI QENKLYIYST QPHIPSVGCE MIAKIFRTDI DNENSLINYS
210 220 230 240 250
APVTVTISVT KATFGDTFVC NHPNMNYQDL IPTMTKNSIY HDVKRITKIH
260 270 280 290 300
EYINSKKKKK NSTSKIGGIQ IAESKDGFWK ILTKNYQIKL KFGVEGYGVM
310 320 330 340 350
GGTFGNWLID SGFKTVETNY EYQRNGKTIN ATTVASVKPS RKCGTRSPVF
360 370 380 390 400
GQLQFSGEMM VLSHNDILTV FYTEREWALS NAIYAKNFAT DFKRQFEITA
410 420 430 440 450
QSDELLVRTN VVPHTIKNTP GKALMEYSHG GFGQIDTSDY TGMALTFRFR
460 470 480 490 500
CISEDLPEGY YDRDKALAFA NVGLTSFQDR QEANGTYWVY NTSTVGFGSC
510 520 530 540 550
YPKKEFEYDI NVTYTTLLPS DPEFTTGGTN YAQSVTAVLE ESFINLQNQV
560 570 580 590 600
NEMLTRMNIS DLTSGVMSVF SVATSFPQIL DGISDLLKAA SSAFKKVKGK
610 620 630 640 650
VGSVAKRLRG KRYVRLFDEN VSIEETPRFL DSIRSSRRPS ILSNMFNDDE
660 670 680 690 700
TFTALHTLAS RTNSVASDVT YLQPIITTRI ANSTPPVIAP ASSVTYAKLK
710 720 730 740 750
DISKIINAEI DPKSIMEFNQ ISNTISILDS TKKLAQYAVD PDIIDGILNK
760 770 780 790 800
MVGGHARSLF SLKVRKHLLD AVEKDAFVKY NYHDLMGKLL NDRELLDITN
810 820
NLSSQKQFEL AKEFRDLLIN ALA
Length:823
Mass (Da):92,912
Last modified:September 13, 2005 - v1
Checksum:iFD0AA4BA99AEB512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ113899 Genomic RNA. Translation: AAZ03487.1.
RefSeqiYP_392492.1. NC_007550.1.

Genome annotation databases

GeneIDi5076652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ113899 Genomic RNA. Translation: AAZ03487.1 .
RefSeqi YP_392492.1. NC_007550.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5076652.

Family and domain databases

InterProi IPR000416. Haemagglutinin_VP4.
[Graphical view ]
Pfami PF00426. VP4_haemagglut. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
    Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
    J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP4_ROTJ1
AccessioniPrimary (citable) accession number: Q45UF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3