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Q45UF8 (VP4_ROTJ1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19)) [Reference proteome]
Taxonomic identifier335103 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length823 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion By similarity. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 823823Outer capsid protein VP4
PRO_0000369845
Chain1 – 245245Outer capsid protein VP8* Potential
PRO_0000369846
Chain260 – 823564Outer capsid protein VP5* Potential
PRO_0000369847

Regions

Compositional bias256 – 2605Poly-Lys

Sites

Site245 – 2462Cleavage By similarity
Site259 – 2602Cleavage By similarity

Amino acid modifications

Glycosylation1431N-linked (GlcNAc...); by host Potential
Glycosylation1531N-linked (GlcNAc...); by host Potential
Glycosylation1981N-linked (GlcNAc...); by host Potential
Glycosylation2611N-linked (GlcNAc...); by host Potential
Glycosylation3301N-linked (GlcNAc...); by host Potential
Glycosylation4841N-linked (GlcNAc...); by host Potential
Glycosylation4911N-linked (GlcNAc...); by host Potential
Glycosylation5111N-linked (GlcNAc...); by host Potential
Glycosylation5581N-linked (GlcNAc...); by host Potential
Glycosylation6201N-linked (GlcNAc...); by host Potential
Glycosylation8011N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q45UF8 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: FD0AA4BA99AEB512

FASTA82392,912
        10         20         30         40         50         60 
MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RIEKDGEKAY CFKNLDPVRY 

        70         80         90        100        110        120 
DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL KADTDIDLSD DVKDDLKRQI MEYYQQGYRA 

       130        140        150        160        170        180 
IFLVRPQNQE QHYIASYSST NLNFTSQPSI GVNLSILNKI QENKLYIYST QPHIPSVGCE 

       190        200        210        220        230        240 
MIAKIFRTDI DNENSLINYS APVTVTISVT KATFGDTFVC NHPNMNYQDL IPTMTKNSIY 

       250        260        270        280        290        300 
HDVKRITKIH EYINSKKKKK NSTSKIGGIQ IAESKDGFWK ILTKNYQIKL KFGVEGYGVM 

       310        320        330        340        350        360 
GGTFGNWLID SGFKTVETNY EYQRNGKTIN ATTVASVKPS RKCGTRSPVF GQLQFSGEMM 

       370        380        390        400        410        420 
VLSHNDILTV FYTEREWALS NAIYAKNFAT DFKRQFEITA QSDELLVRTN VVPHTIKNTP 

       430        440        450        460        470        480 
GKALMEYSHG GFGQIDTSDY TGMALTFRFR CISEDLPEGY YDRDKALAFA NVGLTSFQDR 

       490        500        510        520        530        540 
QEANGTYWVY NTSTVGFGSC YPKKEFEYDI NVTYTTLLPS DPEFTTGGTN YAQSVTAVLE 

       550        560        570        580        590        600 
ESFINLQNQV NEMLTRMNIS DLTSGVMSVF SVATSFPQIL DGISDLLKAA SSAFKKVKGK 

       610        620        630        640        650        660 
VGSVAKRLRG KRYVRLFDEN VSIEETPRFL DSIRSSRRPS ILSNMFNDDE TFTALHTLAS 

       670        680        690        700        710        720 
RTNSVASDVT YLQPIITTRI ANSTPPVIAP ASSVTYAKLK DISKIINAEI DPKSIMEFNQ 

       730        740        750        760        770        780 
ISNTISILDS TKKLAQYAVD PDIIDGILNK MVGGHARSLF SLKVRKHLLD AVEKDAFVKY 

       790        800        810        820 
NYHDLMGKLL NDRELLDITN NLSSQKQFEL AKEFRDLLIN ALA 

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References

[1]"Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ113899 Genomic RNA. Translation: AAZ03487.1.
RefSeqYP_392492.1. NC_007550.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5076652.

Family and domain databases

InterProIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamPF00426. VP4_haemagglut. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP4_ROTJ1
AccessionPrimary (citable) accession number: Q45UF8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families