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Q45UF8

- VP4_ROTJ1

UniProt

Q45UF8 - VP4_ROTJ1

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei245 – 2462CleavageBy similarity
    Sitei259 – 2602CleavageBy similarity

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
    Taxonomic identifieri335103 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007663: Genome

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 823823Outer capsid protein VP4PRO_0000369845Add
    BLAST
    Chaini1 – 245245Outer capsid protein VP8*Sequence AnalysisPRO_0000369846Add
    BLAST
    Chaini260 – 823564Outer capsid protein VP5*Sequence AnalysisPRO_0000369847Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi143 – 1431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi198 – 1981N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi261 – 2611N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi330 – 3301N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi484 – 4841N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi511 – 5111N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi558 – 5581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi620 – 6201N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi801 – 8011N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi256 – 2605Poly-Lys

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Family and domain databases

    InterProiIPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q45UF8-1 [UniParc]FASTAAdd to Basket

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    MSLRSLLITT EAVGETTQTS DHQTSFSTRT YNEINDRPSL RIEKDGEKAY    50
    CFKNLDPVRY DTRMGEYPFD YGGQSTENNQ LQFDLFTKDL KADTDIDLSD 100
    DVKDDLKRQI MEYYQQGYRA IFLVRPQNQE QHYIASYSST NLNFTSQPSI 150
    GVNLSILNKI QENKLYIYST QPHIPSVGCE MIAKIFRTDI DNENSLINYS 200
    APVTVTISVT KATFGDTFVC NHPNMNYQDL IPTMTKNSIY HDVKRITKIH 250
    EYINSKKKKK NSTSKIGGIQ IAESKDGFWK ILTKNYQIKL KFGVEGYGVM 300
    GGTFGNWLID SGFKTVETNY EYQRNGKTIN ATTVASVKPS RKCGTRSPVF 350
    GQLQFSGEMM VLSHNDILTV FYTEREWALS NAIYAKNFAT DFKRQFEITA 400
    QSDELLVRTN VVPHTIKNTP GKALMEYSHG GFGQIDTSDY TGMALTFRFR 450
    CISEDLPEGY YDRDKALAFA NVGLTSFQDR QEANGTYWVY NTSTVGFGSC 500
    YPKKEFEYDI NVTYTTLLPS DPEFTTGGTN YAQSVTAVLE ESFINLQNQV 550
    NEMLTRMNIS DLTSGVMSVF SVATSFPQIL DGISDLLKAA SSAFKKVKGK 600
    VGSVAKRLRG KRYVRLFDEN VSIEETPRFL DSIRSSRRPS ILSNMFNDDE 650
    TFTALHTLAS RTNSVASDVT YLQPIITTRI ANSTPPVIAP ASSVTYAKLK 700
    DISKIINAEI DPKSIMEFNQ ISNTISILDS TKKLAQYAVD PDIIDGILNK 750
    MVGGHARSLF SLKVRKHLLD AVEKDAFVKY NYHDLMGKLL NDRELLDITN 800
    NLSSQKQFEL AKEFRDLLIN ALA 823
    Length:823
    Mass (Da):92,912
    Last modified:September 13, 2005 - v1
    Checksum:iFD0AA4BA99AEB512
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ113899 Genomic RNA. Translation: AAZ03487.1.
    RefSeqiYP_392492.1. NC_007550.1.

    Genome annotation databases

    GeneIDi5076652.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ113899 Genomic RNA. Translation: AAZ03487.1 .
    RefSeqi YP_392492.1. NC_007550.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5076652.

    Family and domain databases

    InterProi IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
      Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
      J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP4_ROTJ1
    AccessioniPrimary (citable) accession number: Q45UF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3