Q45UF7 (VP3_ROTJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Protein VP3 Including the following 2 domains: mRNA guanylyltransferase EC=2.7.7.50 mRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.56
Organism	Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifier	335103 [NCBI]
Taxonomic lineage	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with VP1 Potential. Interacts with VP2 Potential.
Subcellular location	Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.
Sequence similarities	Belongs to the rotavirus VP3 family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Virion
Ligand	GTP-binding Nucleotide-binding RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Protein VP3		PRO_0000369837

Sequences

	Sequence		Length	Mass (Da)
	Q45UF7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F896921F3AC92D24	FASTA	719	84,711
	10 20 30 40 50 60 MAKLIIINSE KGEKVETHED IFKLSNLQQR EIYAITNERT KSILLNQTFY TILDIENEPK 70 80 90 100 110 120 DKVAFDSYNS LFPTSIFSYS RQDRLFGTCN HVLDNNIHYS FALFDSMVDN LSTYLPNDWN 130 140 150 160 170 180 IIKISDSIDY PIGNDLLFYV FDNLVHMTID QFVNSEEKQM NTVPKCKESQ DKIKEVFTDI 190 200 210 220 230 240 MSHLYMPAID YDPQSYNYRV SRREIGNLVR DQVFSLVKGH IHLIGPEMES LRNIIMFLHA 250 260 270 280 290 300 GNSITFHTID TSTKSSYIKE LEFNKKTKLT MASVLVNQRK NMNNFFKGLI RHYITYGIPR 310 320 330 340 350 360 KVYYIGAYPS YWLELITWVP FNIVAYDPKY RRVDNDKIIW YDRLFDRNDI ETIESKSYIY 370 380 390 400 410 420 IDIRTDVRNL DTTKKQRIFK EEDDMIVDMA IKLASKQCTV MFKRKIFPGN NMSFGDPLFH 430 440 450 460 470 480 PKLTQLGREY YNCITTIVSP SVYKESELYS LLLSARSNNV SNYVYGGSKF DQFSIVNCNS 490 500 510 520 530 540 TVVALYSLSN TVNSLKTIEH AIKYNHIITF PHRTDRGDWR NIEELDNSSP FQNRKRQLEF 550 560 570 580 590 600 EDWSIDPKNY VMKFRCEMVS ESVFLQLGHS RALIPDLYNH MISLRMEMPL FYSDRFFSHI 610 620 630 640 650 660 GIRQPSIFKR DSYMTSRLSA YISRQLTHSI NLSVLKRNHF EGYSGHLIAI ETSFSSLVFT 670 680 690 700 710 MSPYRWLIRA KKLLTKNKMR DKFKIGDGQP HTREEFENTY DYLKINRLVN STFRSLLLD

References

[1]

"Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses." Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S. J. Gen. Virol. 89:2622-2629(2008) [PubMed: 18796732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ113900 Genomic RNA. Translation: AAZ03488.1.
RefSeq	YP_392493.1. NC_007551.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5076653.
Family and domain databases
InterPro	IPR009697. Rotavirus_VP3. [Graphical view]
Pfam	PF06929. Rotavirus_VP3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VP3_ROTJ1

Accession

Primary (citable) accession number: Q45UF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 13, 2005
Last modified:	September 21, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program