Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q45UF7 (VP3_ROTJ1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein VP3

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
OrganismRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19)) [Reference proteome]
Taxonomic identifier335103 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with VP1 Potential. Interacts with VP2 Potential.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.

Sequence similarities

Belongs to the rotavirus VP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 719719Protein VP3
PRO_0000369837

Sequences

Sequence LengthMass (Da)Tools
Q45UF7 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: F896921F3AC92D24

FASTA71984,711
        10         20         30         40         50         60 
MAKLIIINSE KGEKVETHED IFKLSNLQQR EIYAITNERT KSILLNQTFY TILDIENEPK 

        70         80         90        100        110        120 
DKVAFDSYNS LFPTSIFSYS RQDRLFGTCN HVLDNNIHYS FALFDSMVDN LSTYLPNDWN 

       130        140        150        160        170        180 
IIKISDSIDY PIGNDLLFYV FDNLVHMTID QFVNSEEKQM NTVPKCKESQ DKIKEVFTDI 

       190        200        210        220        230        240 
MSHLYMPAID YDPQSYNYRV SRREIGNLVR DQVFSLVKGH IHLIGPEMES LRNIIMFLHA 

       250        260        270        280        290        300 
GNSITFHTID TSTKSSYIKE LEFNKKTKLT MASVLVNQRK NMNNFFKGLI RHYITYGIPR 

       310        320        330        340        350        360 
KVYYIGAYPS YWLELITWVP FNIVAYDPKY RRVDNDKIIW YDRLFDRNDI ETIESKSYIY 

       370        380        390        400        410        420 
IDIRTDVRNL DTTKKQRIFK EEDDMIVDMA IKLASKQCTV MFKRKIFPGN NMSFGDPLFH 

       430        440        450        460        470        480 
PKLTQLGREY YNCITTIVSP SVYKESELYS LLLSARSNNV SNYVYGGSKF DQFSIVNCNS 

       490        500        510        520        530        540 
TVVALYSLSN TVNSLKTIEH AIKYNHIITF PHRTDRGDWR NIEELDNSSP FQNRKRQLEF 

       550        560        570        580        590        600 
EDWSIDPKNY VMKFRCEMVS ESVFLQLGHS RALIPDLYNH MISLRMEMPL FYSDRFFSHI 

       610        620        630        640        650        660 
GIRQPSIFKR DSYMTSRLSA YISRQLTHSI NLSVLKRNHF EGYSGHLIAI ETSFSSLVFT 

       670        680        690        700        710 
MSPYRWLIRA KKLLTKNKMR DKFKIGDGQP HTREEFENTY DYLKINRLVN STFRSLLLD 

« Hide

References

[1]"Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ113900 Genomic RNA. Translation: AAZ03488.1.
RefSeqYP_392493.1. NC_007551.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5076653.

Family and domain databases

InterProIPR011181. VP3_Rotav.
[Graphical view]
PfamPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP3_ROTJ1
AccessionPrimary (citable) accession number: Q45UF7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families