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Q45UF4

- NSP2_ROTJ1

UniProt

Q45UF4 - NSP2_ROTJ1

Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei231 – 2311For NTPase activityBy similarity

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW
    2. nucleotide binding Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 2 (EC:3.6.4.-)
    Short name:
    NSP2
    OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
    Taxonomic identifieri335103 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007663: Genome

    Subcellular locationi

    Host cytoplasm Curated
    Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Non-structural protein 2PRO_0000369853Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 24736RNA-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus NSP2 family.Curated

    Sequencei

    Sequence statusi: Complete.

    Q45UF4-1 [UniParc]FASTAAdd to Basket

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    MVSIKVSLAD FIVKTEEGWI PSDNCPALDR FKTKTEKELL DSIKKEGADR    50
    ASIRKQLFLT SISNKRLTQL GGVPVRDIRT STTIPSSTRN LITDWLLNIF 100
    NDEESGEEVE SAIASKYPDI FCSADKISRV AQRLENRRDR VHEDGFRILS 150
    ATMLAIDSDI ATEGKCEIVR ATEDAIIAKF EPVSEHLCIG NPRGVFYKAF 200
    PIKKEQPMVY GVKALLGISN RDFIMNHGHG HLRTVPYSEI NNAVRSFAKK 250
    NEAEIKRIRS DSLSPNAGEK FINMCDMLLQ KEKIETVIAK IMKSDKN 297
    Length:297
    Mass (Da):33,433
    Last modified:September 13, 2005 - v1
    Checksum:i4F16788D9EA04819
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ113903 Genomic RNA. Translation: AAZ03491.1.
    RefSeqiYP_392496.1. NC_007554.1.

    Genome annotation databases

    GeneIDi5076656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ113903 Genomic RNA. Translation: AAZ03491.1 .
    RefSeqi YP_392496.1. NC_007554.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5076656.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
      Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
      J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNSP2_ROTJ1
    AccessioniPrimary (citable) accession number: Q45UF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3