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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors (By similarity).By similarity

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus B (isolate Human/China/ADRV-N J19/1997) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))
Taxonomic identifieri335103 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007663: Genome

Subcellular locationi

Virion Curated. Host rough endoplasmic reticulum membrane Curated; Single-pass membrane protein Curated; Lumenal side Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – ?1313HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. T=13 icosahedral viral capsid Source: UniProtKB-KW
  4. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Host membrane, Membrane, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Outer capsid glycoprotein VP7PRO_0000369863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008818. Rotavirus_VP7.
[Graphical view]
PfamiPF05868. Rotavirus_VP7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45UF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFIILSVG VDAISYIQND RSNDLCIVYE MTSFGTSFNN ANDSLVKLHK
60 70 80 90 100
HMGLKYEVCK IESNANALTQ MQKCNCIYDD TPQIVVFTNF KKSSLKTLIG
110 120 130 140 150
TENKCELLPQ TTIYTPTVDI ESEYFIYGND VKICYLDKNL LGIGCDATDT
160 170 180 190 200
TSWLDLDAGL PTNHALDIPE ITSDGFKLFA KYSDSFLCQR LMDEPKKQIQ
210 220 230 240 250
FYAEVDNVPS NDVIESSRSW ASVWKVVKTV LHFTYHILDL FYGNRRATAR

MIEHSPLG
Length:258
Mass (Da):29,265
Last modified:September 13, 2005 - v1
Checksum:i55DFEC621B61DBB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ113905 Genomic RNA. Translation: AAZ03493.1.
RefSeqiYP_392498.1. NC_007556.1.

Genome annotation databases

GeneIDi5076658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ113905 Genomic RNA. Translation: AAZ03493.1.
RefSeqiYP_392498.1. NC_007556.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5076658.

Family and domain databases

InterProiIPR008818. Rotavirus_VP7.
[Graphical view]
PfamiPF05868. Rotavirus_VP7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses."
    Jiang S., Ji S., Tang Q., Cui X., Yang H., Kan B., Gao S.
    J. Gen. Virol. 89:2622-2629(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP7_ROTJ1
AccessioniPrimary (citable) accession number: Q45UF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 13, 2005
Last modified: January 7, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.